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Protein

Cellulose synthase A catalytic subunit 4 [UDP-forming]

Gene

CESA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the secondary cell wall formation. Required for the xylem cell wall thickening.2 Publications

Catalytic activityi

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: plant cellulose biosynthesis

This protein is involved in the pathway plant cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway plant cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Zinc 1By similarity1
Metal bindingi26Zinc 1By similarity1
Metal bindingi42Zinc 2By similarity1
Metal bindingi45Zinc 2By similarity1
Metal bindingi50Zinc 1By similarity1
Metal bindingi53Zinc 1By similarity1
Metal bindingi65Zinc 2By similarity1
Metal bindingi68Zinc 2By similarity1
Active sitei335Sequence analysis1
Binding sitei501SubstrateSequence analysis1
Binding sitei503SubstrateSequence analysis1
Active sitei748Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri23 – 69RING-type; degenerateAdd BLAST47

GO - Molecular functioni

GO - Biological processi

  • cellulose biosynthetic process Source: TAIR
  • cell wall organization Source: UniProtKB-KW
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • plant-type secondary cell wall biogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation, Cellulose biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2364.
BRENDAi2.4.1.12. 399.
UniPathwayiUPA00695.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.
TCDBi4.D.3.1.7. the glycan glucosyl transferase (opgh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cellulose synthase A catalytic subunit 4 [UDP-forming] (EC:2.4.1.12)
Short name:
AtCesA4
Alternative name(s):
Protein IRREGULAR XYLEM 5
Short name:
AtIRX5
Gene namesi
Name:CESA4
Synonyms:IRX5
Ordered Locus Names:At5g44030
ORF Names:MRH10.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G44030.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 215CytoplasmicSequence analysisAdd BLAST215
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 239ExtracellularSequence analysis3
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 831CytoplasmicSequence analysisAdd BLAST571
Transmembranei832 – 852HelicalSequence analysisAdd BLAST21
Topological domaini853 – 857ExtracellularSequence analysis5
Transmembranei858 – 878HelicalSequence analysisAdd BLAST21
Topological domaini879 – 895CytoplasmicSequence analysisAdd BLAST17
Transmembranei896 – 916HelicalSequence analysisAdd BLAST21
Topological domaini917 – 945ExtracellularSequence analysisAdd BLAST29
Transmembranei946 – 966HelicalSequence analysisAdd BLAST21
Topological domaini967 – 977CytoplasmicSequence analysisAdd BLAST11
Transmembranei978 – 998HelicalSequence analysisAdd BLAST21
Topological domaini999 – 1007ExtracellularSequence analysis9
Transmembranei1008 – 1028HelicalSequence analysisAdd BLAST21
Topological domaini1029 – 1049CytoplasmicSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Enhanced resistance to the pathogens Ralstonia solanacearum and Plectosphaerella cucumerina.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi989 – 1049Missing in irx5-2; reduced levels of crystalline cellulose in secondary cell wall, dwarf and dark green, with irregular xylems and thinner cell walls in xylem and interfascicular tissues. 1 PublicationAdd BLAST61

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001663701 – 1049Cellulose synthase A catalytic subunit 4 [UDP-forming]Add BLAST1049

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135Phosphoserine1 Publication1
Glycosylationi921N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

S-acylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ84JA6.
PRIDEiQ84JA6.

PTM databases

iPTMnetiQ84JA6.

Expressioni

Tissue specificityi

Confined to secondary cell wall developing tissues such as xylems and interfascicular regions. Expressed in roots, hypocotyls, leaves, inflorescences and flowers.3 Publications

Developmental stagei

Not expressed in embryos. In young leaves, localized in transient patches along the vascular system. In young inflorescence stems, observed in vascular bundles of primary xylems. In maturing inflorescence stems, most pronounced in regions of developing interfascicular fibers.1 Publication

Gene expression databases

ExpressionAtlasiQ84JA6. baseline and differential.
GenevisibleiQ84JA6. AT.

Interactioni

Subunit structurei

Interacts with CESA7 and CESA8. Assembly with CESA7 and CESA8 is required for functional complex and localization in secondary cell wall deposition sites. Interacts with STL1 and STL2, but not with GOT1 (PubMed:27277162).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CESA7Q9SWW66EBI-8579072,EBI-4477361
CESA8Q8LPK54EBI-8579072,EBI-8579199

Protein-protein interaction databases

BioGridi19676. 7 interactors.
IntActiQ84JA6. 2 interactors.
MINTiMINT-6950789.
STRINGi3702.AT5G44030.1.

Structurei

3D structure databases

ProteinModelPortaliQ84JA6.
SMRiQ84JA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili389 – 416Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi597 – 608Cys-richAdd BLAST12
Compositional biasi616 – 644Lys-richAdd BLAST29

Sequence similaritiesi

Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri23 – 69RING-type; degenerateAdd BLAST47

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IRAS. Eukaryota.
COG1215. LUCA.
HOGENOMiHOG000241942.
InParanoidiQ84JA6.
KOiK10999.
OMAiINDLWRN.
OrthoDBiEOG093601AL.
PhylomeDBiQ84JA6.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR005150. Cellulose_synth.
IPR027934. CES_Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF03552. Cellulose_synt. 1 hit.
PF14569. zf-UDP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84JA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPNTMASFD DEHRHSSFSA KICKVCGDEV KDDDNGQTFV ACHVCVYPVC
60 70 80 90 100
KPCYEYERSN GNKCCPQCNT LYKRHKGSPK IAGDEENNGP DDSDDELNIK
110 120 130 140 150
YRQDGSSIHQ NFAYGSENGD YNSKQQWRPN GRAFSSTGSV LGKDFEAERD
160 170 180 190 200
GYTDAEWKER VDKWKARQEK RGLVTKGEQT NEDKEDDEEE YLDAEARQPL
210 220 230 240 250
WRKVPISSSK ISPYRIVIVL RLVILVFFFR FRILTPAKDA YPLWLISVIC
260 270 280 290 300
EIWFALSWIL DQFPKWFPIN RETYLDRLSM RFERDGEKNK LAPVDVFVST
310 320 330 340 350
VDPLKEPPII TANTILSILA VDYPVNKVSC YVSDDGASML LFDTLSETSE
360 370 380 390 400
FARRWVPFCK KYNVEPRAPE FYFSEKIDYL KDKVQTTFVK DRRAMKREYE
410 420 430 440 450
EFKVRINALV AKAQKKPEEG WVMQDGTPWP GNNTRDHPGM IQVYLGKEGA
460 470 480 490 500
FDIDGNELPR LVYVSREKRP GYAHHKKAGA MNAMVRVSAV LTNAPFMLNL
510 520 530 540 550
DCDHYINNSK AIRESMCFLM DPQLGKKLCY VQFPQRFDGI DLNDRYANRN
560 570 580 590 600
IVFFDINMRG LDGIQGPVYV GTGCVFNRPA LYGYEPPVSE KRKKMTCDCW
610 620 630 640 650
PSWICCCCGG GNRNHKSDSS KKKSGIKSLF SKLKKKTKKK SDDKTMSSYS
660 670 680 690 700
RKRSSTEAIF DLEDIEEGLE GYDELEKSSL MSQKNFEKRF GMSPVFIAST
710 720 730 740 750
LMENGGLPEA TNTSSLIKEA IHVISCGYEE KTEWGKEIGW IYGSVTEDIL
760 770 780 790 800
TGFRMHCRGW KSVYCMPKRP AFKGSAPINL SDRLHQVLRW ALGSVEIFFS
810 820 830 840 850
RHCPLWYAWG GKLKILERLA YINTIVYPFT SIPLLAYCTI PAVCLLTGKF
860 870 880 890 900
IIPTINNFAS IWFLALFLSI IATAILELRW SGVSINDLWR NEQFWVIGGV
910 920 930 940 950
SAHLFAVFQG LLKVLFGVDT NFTVTSKGAS DEADEFGDLY LFKWTTLLIP
960 970 980 990 1000
PTTLIILNMV GVVAGVSDAI NNGYGSWGPL FGKLFFAFWV IVHLYPFLKG
1010 1020 1030 1040
LMGRQNRTPT IVVLWSILLA SIFSLVWVRI DPFLPKQTGP LLKQCGVDC
Length:1,049
Mass (Da):119,599
Last modified:June 1, 2003 - v1
Checksum:iD6D2B1B73BBC3E41
GO

Sequence cautioni

The sequence BAB09063 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127W → C in AAO15532 (PubMed:12538856).Curated1
Sequence conflicti191Y → EL in AAO15532 (PubMed:12538856).Curated1
Sequence conflicti542L → H in AAO15532 (PubMed:12538856).Curated1
Sequence conflicti616 – 618KSD → HKSKSSDS in AAO15532 (PubMed:12538856).Curated3
Sequence conflicti630F → L in AAO15532 (PubMed:12538856).Curated1
Sequence conflicti637T → N in AAO15532 (PubMed:12538856).Curated1
Sequence conflicti655S → A in AAO15532 (PubMed:12538856).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF458083 mRNA. Translation: AAO15532.1.
AB006703 Genomic DNA. Translation: BAB09063.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95050.1.
BT005710 mRNA. Translation: AAO64130.1.
BT006111 mRNA. Translation: AAP04096.1.
AK228561 mRNA. Translation: BAF00480.1.
RefSeqiNP_199216.2. NM_123770.4.
UniGeneiAt.49129.
At.71068.

Genome annotation databases

EnsemblPlantsiAT5G44030.1; AT5G44030.1; AT5G44030.
GeneIDi834426.
GrameneiAT5G44030.1; AT5G44030.1; AT5G44030.
KEGGiath:AT5G44030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF458083 mRNA. Translation: AAO15532.1.
AB006703 Genomic DNA. Translation: BAB09063.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95050.1.
BT005710 mRNA. Translation: AAO64130.1.
BT006111 mRNA. Translation: AAP04096.1.
AK228561 mRNA. Translation: BAF00480.1.
RefSeqiNP_199216.2. NM_123770.4.
UniGeneiAt.49129.
At.71068.

3D structure databases

ProteinModelPortaliQ84JA6.
SMRiQ84JA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19676. 7 interactors.
IntActiQ84JA6. 2 interactors.
MINTiMINT-6950789.
STRINGi3702.AT5G44030.1.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.
TCDBi4.D.3.1.7. the glycan glucosyl transferase (opgh) family.

PTM databases

iPTMnetiQ84JA6.

Proteomic databases

PaxDbiQ84JA6.
PRIDEiQ84JA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G44030.1; AT5G44030.1; AT5G44030.
GeneIDi834426.
GrameneiAT5G44030.1; AT5G44030.1; AT5G44030.
KEGGiath:AT5G44030.

Organism-specific databases

TAIRiAT5G44030.

Phylogenomic databases

eggNOGiENOG410IRAS. Eukaryota.
COG1215. LUCA.
HOGENOMiHOG000241942.
InParanoidiQ84JA6.
KOiK10999.
OMAiINDLWRN.
OrthoDBiEOG093601AL.
PhylomeDBiQ84JA6.

Enzyme and pathway databases

UniPathwayiUPA00695.
BioCyciMetaCyc:MONOMER-2364.
BRENDAi2.4.1.12. 399.

Miscellaneous databases

PROiQ84JA6.

Gene expression databases

ExpressionAtlasiQ84JA6. baseline and differential.
GenevisibleiQ84JA6. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR005150. Cellulose_synth.
IPR027934. CES_Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF03552. Cellulose_synt. 1 hit.
PF14569. zf-UDP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCESA4_ARATH
AccessioniPrimary (citable) accession number: Q84JA6
Secondary accession number(s): Q0WQW9, Q8GZN8, Q9FNC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.