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Protein

Laccase-15

Gene

TT10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products (By similarity). Involved in lignin synthesis in seed coats, in seed coat permeability, in seed germination, and in root elongation. Required for the seed coat (testa) brown pigmentation by mediating the polymerization of proanthocyanidin (tannin) from its monomer precursor epicatechin. Promotes sligthly seed dormancy.By similarity5 Publications

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Copper 1; type 2By similarity1
Metal bindingi79Copper 2; type 3By similarity1
Metal bindingi122Copper 2; type 3By similarity1
Metal bindingi124Copper 3; type 3By similarity1
Metal bindingi462Copper 4; type 1By similarity1
Metal bindingi465Copper 1; type 2By similarity1
Metal bindingi467Copper 3; type 3By similarity1
Metal bindingi526Copper 3; type 3By similarity1
Metal bindingi527Copper 4; type 1By similarity1
Metal bindingi528Copper 2; type 3By similarity1
Metal bindingi532Copper 4; type 1By similarity1
Metal bindingi537Copper 4; type 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • lignin biosynthetic process Source: TAIR
  • lignin catabolic process Source: UniProtKB-KW
  • proanthocyanidin biosynthetic process Source: TAIR
  • response to copper ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G48100-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-15 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 15
Diphenol oxidase 15
Protein TRANSPARENT TESTA 10
Urishiol oxidase 15
Gene namesi
Name:TT10
Synonyms:LAC15
Ordered Locus Names:At5g48100
ORF Names:MDN11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G48100.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi152D → G in tt10-1; transparent testa; when associated with D-509. 1 Publication1
Mutagenesisi509G → D in tt10-1; transparent testa; when associated with G-152. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000028364322 – 565Laccase-15Add BLAST544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi73N-linked (GlcNAc...)Sequence analysis1
Glycosylationi182N-linked (GlcNAc...)Sequence analysis1
Glycosylationi234N-linked (GlcNAc...)Sequence analysis1
Glycosylationi293N-linked (GlcNAc...)Sequence analysis1
Glycosylationi324N-linked (GlcNAc...)Sequence analysis1
Glycosylationi372N-linked (GlcNAc...)Sequence analysis1
Glycosylationi382N-linked (GlcNAc...)Sequence analysis1
Glycosylationi418N-linked (GlcNAc...)Sequence analysis1
Glycosylationi482N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ84J37.

Expressioni

Tissue specificityi

Mostly expressed in siliques, particularly in developing seeds. Also detected at low levels in stems, seedlings, and flowers.4 Publications

Developmental stagei

Transcript levels increase during rosette leaves development. In the inflorescence stem, highest levels in the young, developing tip and lowest levels in the basal stem tissues. Strong increase 4 days after fertilization. Specifically localized in developing seed coat (testa). Detected in the endothelium and in the pigment strand at the chalaza zone during early stages of embryo morphogenesis. Later, the activity increased and spread to the outer integument, mostly in the oil penultimate cell layer. Strongly expressed in early aborted seeds and in the transmitting tissue of the silique.2 Publications

Gene expression databases

GenevisibleiQ84J37. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G48100.1.

Structurei

3D structure databases

ProteinModelPortaliQ84J37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 143Plastocyanin-like 1Add BLAST117
Domaini153 – 305Plastocyanin-like 2Add BLAST153
Domaini408 – 547Plastocyanin-like 3Add BLAST140

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
HOGENOMiHOG000241916.
InParanoidiQ84J37.
KOiK05909.
OMAiATYITIS.
OrthoDBiEOG093606A1.
PhylomeDBiQ84J37.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84J37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSFFNLFL ISLFLYNNCI AHHYTFTVRE VPYTKLCSTK AILTVNSQFP
60 70 80 90 100
GPIIKVHKGD TIYVNVQNRA SENITMHWHG VEQPRNPWSD GPEYITQCPI
110 120 130 140 150
RPGSDFLYKV IFSIEDTTVW WHAHSSWTRA TVHGLIFVYP RPPQILPFPK
160 170 180 190 200
ADHEVPIILG EWWKRDVREV VEEFVRTGGA PNVSDALTIN GHPGFLYPCS
210 220 230 240 250
KSDTFHLTVE KGKTYRIRMV NAAMNLPLFF AIANHSLTVV SADGHYIKPI
260 270 280 290 300
KATYITISPG ETLDMLLHAD QDPERTYYMA ARAYQSGNID FNNSTTIGIL
310 320 330 340 350
SYTSSCKAKT SSFSGYYPTL PFYNDTSAAF GFFTKIKCLF SGQVPVQISR
360 370 380 390 400
RIITTVSINL RMCPQNSCEG PNGSRLAASM NNISFVTPSH VDILKAYYYH
410 420 430 440 450
IKGVYGTRFP EFPPLIFNFT AENQPLFLET PRLATEVKVI EFGQVVELVI
460 470 480 490 500
QGTSLVGGGL DHPMHLHGFS FYVVGVGFGN YNISEEDPSS RYNLYDPPYK
510 520 530 540 550
NTMTVPRNGW IAIRFVADNP GVWFMHCHLD RHQTWGMNVV FIVKNGREPN
560
QQILPPPDDL PPCYE
Length:565
Mass (Da):63,994
Last modified:June 1, 2003 - v1
Checksum:iFDBCBE3D6F10DBA4
GO

Sequence cautioni

The sequence BAB11074 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017064 Genomic DNA. Translation: BAB11074.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95619.1.
BT002919 mRNA. Translation: AAO22735.1.
BT005152 mRNA. Translation: AAO50685.1.
RefSeqiNP_199621.2. NM_124184.2.
UniGeneiAt.9463.

Genome annotation databases

EnsemblPlantsiAT5G48100.1; AT5G48100.1; AT5G48100.
GeneIDi834862.
GrameneiAT5G48100.1; AT5G48100.1; AT5G48100.
KEGGiath:AT5G48100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017064 Genomic DNA. Translation: BAB11074.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95619.1.
BT002919 mRNA. Translation: AAO22735.1.
BT005152 mRNA. Translation: AAO50685.1.
RefSeqiNP_199621.2. NM_124184.2.
UniGeneiAt.9463.

3D structure databases

ProteinModelPortaliQ84J37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G48100.1.

Proteomic databases

PaxDbiQ84J37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G48100.1; AT5G48100.1; AT5G48100.
GeneIDi834862.
GrameneiAT5G48100.1; AT5G48100.1; AT5G48100.
KEGGiath:AT5G48100.

Organism-specific databases

TAIRiAT5G48100.

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
HOGENOMiHOG000241916.
InParanoidiQ84J37.
KOiK05909.
OMAiATYITIS.
OrthoDBiEOG093606A1.
PhylomeDBiQ84J37.

Enzyme and pathway databases

BioCyciARA:AT5G48100-MONOMER.

Miscellaneous databases

PROiQ84J37.

Gene expression databases

GenevisibleiQ84J37. AT.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLAC15_ARATH
AccessioniPrimary (citable) accession number: Q84J37
Secondary accession number(s): Q9FI28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.