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Q84J37

- LAC15_ARATH

UniProt

Q84J37 - LAC15_ARATH

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Protein
Laccase-15
Gene
TT10, LAC15, At5g48100, MDN11.18
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products By similarity. Involved in lignin synthesis in seed coats, in seed coat permeability, in seed germination, and in root elongation. Required for the seed coat (testa) brown pigmentation by mediating the polymerization of proanthocyanidin (tannin) from its monomer precursor epicatechin. Promotes sligthly seed dormancy.5 Publications

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Binds 4 copper ions per monomer By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Copper 1; type 2 By similarity
Metal bindingi79 – 791Copper 2; type 3 By similarity
Metal bindingi122 – 1221Copper 2; type 3 By similarity
Metal bindingi124 – 1241Copper 3; type 3 By similarity
Metal bindingi462 – 4621Copper 4; type 1 By similarity
Metal bindingi465 – 4651Copper 1; type 2 By similarity
Metal bindingi467 – 4671Copper 3; type 3 By similarity
Metal bindingi526 – 5261Copper 3; type 3 By similarity
Metal bindingi527 – 5271Copper 4; type 1 By similarity
Metal bindingi528 – 5281Copper 2; type 3 By similarity
Metal bindingi532 – 5321Copper 4; type 1 By similarity
Metal bindingi537 – 5371Copper 4; type 1 By similarity

GO - Molecular functioni

  1. copper ion binding Source: TAIR
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin biosynthetic process Source: TAIR
  2. lignin catabolic process Source: UniProtKB-KW
  3. proanthocyanidin biosynthetic process Source: TAIR
  4. response to copper ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G48100-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-15 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 15
Diphenol oxidase 15
Protein TRANSPARENT TESTA 10
Urishiol oxidase 15
Gene namesi
Name:TT10
Synonyms:LAC15
Ordered Locus Names:At5g48100
ORF Names:MDN11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G48100.

Subcellular locationi

Secretedextracellular spaceapoplast Reviewed prediction

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi152 – 1521D → G in tt10-1; transparent testa; when associated with D-509. 1 Publication
Mutagenesisi509 – 5091G → D in tt10-1; transparent testa; when associated with G-152. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 565544Laccase-15
PRO_0000283643Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi182 – 1821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi234 – 2341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi293 – 2931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi324 – 3241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi372 – 3721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi382 – 3821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi418 – 4181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi482 – 4821N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ84J37.
PRIDEiQ84J37.

Expressioni

Tissue specificityi

Mostly expressed in siliques, particularly in developing seeds. Also detected at low levels in stems, seedlings, and flowers.4 Publications

Developmental stagei

Transcript levels increase during rosette leaves development. In the inflorescence stem, highest levels in the young, developing tip and lowest levels in the basal stem tissues. Strong increase 4 days after fertilization. Specifically localized in developing seed coat (testa). Detected in the endothelium and in the pigment strand at the chalaza zone during early stages of embryo morphogenesis. Later, the activity increased and spread to the outer integument, mostly in the oil penultimate cell layer. Strongly expressed in early aborted seeds and in the transmitting tissue of the silique.2 Publications

Gene expression databases

GenevestigatoriQ84J37.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G48100.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ84J37.
SMRiQ84J37. Positions 23-553.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 143117Plastocyanin-like 1
Add
BLAST
Domaini153 – 305153Plastocyanin-like 2
Add
BLAST
Domaini408 – 547140Plastocyanin-like 3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiQ84J37.
OMAiASENITM.
PhylomeDBiQ84J37.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84J37-1 [UniParc]FASTAAdd to Basket

« Hide

MSHSFFNLFL ISLFLYNNCI AHHYTFTVRE VPYTKLCSTK AILTVNSQFP    50
GPIIKVHKGD TIYVNVQNRA SENITMHWHG VEQPRNPWSD GPEYITQCPI 100
RPGSDFLYKV IFSIEDTTVW WHAHSSWTRA TVHGLIFVYP RPPQILPFPK 150
ADHEVPIILG EWWKRDVREV VEEFVRTGGA PNVSDALTIN GHPGFLYPCS 200
KSDTFHLTVE KGKTYRIRMV NAAMNLPLFF AIANHSLTVV SADGHYIKPI 250
KATYITISPG ETLDMLLHAD QDPERTYYMA ARAYQSGNID FNNSTTIGIL 300
SYTSSCKAKT SSFSGYYPTL PFYNDTSAAF GFFTKIKCLF SGQVPVQISR 350
RIITTVSINL RMCPQNSCEG PNGSRLAASM NNISFVTPSH VDILKAYYYH 400
IKGVYGTRFP EFPPLIFNFT AENQPLFLET PRLATEVKVI EFGQVVELVI 450
QGTSLVGGGL DHPMHLHGFS FYVVGVGFGN YNISEEDPSS RYNLYDPPYK 500
NTMTVPRNGW IAIRFVADNP GVWFMHCHLD RHQTWGMNVV FIVKNGREPN 550
QQILPPPDDL PPCYE 565
Length:565
Mass (Da):63,994
Last modified:June 1, 2003 - v1
Checksum:iFDBCBE3D6F10DBA4
GO

Sequence cautioni

The sequence BAB11074.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017064 Genomic DNA. Translation: BAB11074.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95619.1.
BT002919 mRNA. Translation: AAO22735.1.
BT005152 mRNA. Translation: AAO50685.1.
RefSeqiNP_199621.2. NM_124184.2.
UniGeneiAt.9463.

Genome annotation databases

EnsemblPlantsiAT5G48100.1; AT5G48100.1; AT5G48100.
GeneIDi834862.
KEGGiath:AT5G48100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017064 Genomic DNA. Translation: BAB11074.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED95619.1 .
BT002919 mRNA. Translation: AAO22735.1 .
BT005152 mRNA. Translation: AAO50685.1 .
RefSeqi NP_199621.2. NM_124184.2.
UniGenei At.9463.

3D structure databases

ProteinModelPortali Q84J37.
SMRi Q84J37. Positions 23-553.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G48100.1-P.

Proteomic databases

PaxDbi Q84J37.
PRIDEi Q84J37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G48100.1 ; AT5G48100.1 ; AT5G48100 .
GeneIDi 834862.
KEGGi ath:AT5G48100.

Organism-specific databases

TAIRi AT5G48100.

Phylogenomic databases

eggNOGi COG2132.
HOGENOMi HOG000241916.
InParanoidi Q84J37.
OMAi ASENITM.
PhylomeDBi Q84J37.

Enzyme and pathway databases

BioCyci ARA:AT5G48100-MONOMER.

Gene expression databases

Genevestigatori Q84J37.

Family and domain databases

Gene3Di 2.60.40.420. 3 hits.
InterProi IPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view ]
Pfami PF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 4 hits.
TIGRFAMsi TIGR03389. laccase. 1 hit.
PROSITEi PS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Mutations affecting the testa colour in Arabidopsis."
    Koornneef M.
    Arabidopsis Inf. Serv. 27:1-4(1990)
    Cited for: FUNCTION.
  5. "Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis."
    Debeaujon I., Leon-Kloosterziel K.M., Koornneef M.
    Plant Physiol. 122:403-413(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Erratum
    Debeaujon I., Leon-Kloosterziel K.M., Koornneef M.
    Plant Physiol. 125:1139-1141(2001)
  7. "Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
    McCaig B.C., Meagher R.B., Dean J.F.D.
    Planta 221:619-636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "TRANSPARENT TESTA10 encodes a laccase-like enzyme involved in oxidative polymerization of flavonoids in Arabidopsis seed coat."
    Pourcel L., Routaboul J.-M., Kerhoas L., Caboche M., Lepiniec L., Debeaujon I.
    Plant Cell 17:2966-2980(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-152 AND GLY-509.
  9. "Mutant identification and characterization of the laccase gene family in Arabidopsis."
    Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
    J. Exp. Bot. 57:2563-2569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  10. "Involvement of AtLAC15 in lignin synthesis in seeds and in root elongation of Arabidopsis."
    Liang M., Davis E., Gardner D., Cai X., Wu Y.
    Planta 224:1185-1196(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
    Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
    Plant Physiol. Biochem. 0:0-0(2013)
    Cited for: REVIEW, NOMENCLATURE.

Entry informationi

Entry nameiLAC15_ARATH
AccessioniPrimary (citable) accession number: Q84J37
Secondary accession number(s): Q9FI28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi