Q84HQ2 (Q84HQ2_BIFAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Sucrose phosphorylase EMBL AAO33821.1
EC=2.4.1.7 EMBL AAO33821.1

Gene names

Name:

sucP EMBL AAO33821.1

Organism

Bifidobacterium adolescentis EMBL AAO33821.1

Taxonomic identifier

1680 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosyltransferase EMBL AAO33821.1 Transferase
Technical term	3D-structure PDB 2GDU PDB 1R7A PDB 2GDV
Gene Ontology (GO)
Biological process	sucrose metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro sucrose phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	50 – 53	4	Sucrose binding PDB 2GDU
	Region	190 – 192	3	Sucrose binding PDB 2GDU
	Region	289 – 290	2	Sucrose binding PDB 2GDU
	Region	342 – 345	4	Sucrose binding PDB 2GDU
Sites
	Binding site	88	1	Sucrose PDB 2GDU
	Binding site	156	1	Sucrose PDB 2GDU
	Binding site	399	1	Sucrose PDB 2GDU
Amino acid modifications
	Modified residue	356	1	Cysteine sulfinic acid (-SO2H) PDB 2GDU PDB 1R7A

Sequences

	Sequence		Length	Mass (Da)
	Q84HQ2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 10705FE3182E5754	FASTA	504	56,190
	10 20 30 40 50 60 MKNKVQLITY ADRLGDGTIK SMTDILRTRF DGVYDGVHIL PFFTPFDGAD AGFDPIDHTK 70 80 90 100 110 120 VDERLGSWDD VAELSKTHNI MVDAIVNHMS WESKQFQDVL AKGEESEYYP MFLTMSSVFP 130 140 150 160 170 180 NGATEEDLAG IYRPRPGLPF THYKFAGKTR LVWVSFTPQQ VDIDTDSDKG WEYLMSIFDQ 190 200 210 220 230 240 MAASHVSYIR LDAVGYGAKE AGTSCFMTPK TFKLISRLRE EGVKRGLEIL IEVHSYYKKQ 250 260 270 280 290 300 VEIASKVDRV YDFALPPLLL HALSTGHVEP VAHWTDIRPN NAVTVLDTHD GIGVIDIGSD 310 320 330 340 350 360 QLDRSLKGLV PDEDVDNLVN TIHANTHGES QAATGAAASN LDLYQVNSTY YSALGCNDQH 370 380 390 400 410 420 YIAARAVQFF LPGVPQVYYV GALAGKNDME LLRKTNNGRD INRHYYSTAE IDENLKRPVV 430 440 450 460 470 480 KALNALAKFR NELDAFDGTF SYTTDDDTSI SFTWRGETSQ ATLTFEPKRG LGVDNTTPVA 490 500 MLEWEDSAGD HRSDDLIANP PVVA

References

[1]	"Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083." van den Broek L.A., van Boxtel E.L., Kievit R.P., Verhoef R., Beldman G., Voragen A.G. Appl. Microbiol. Biotechnol. 65:219-227(2004) [PubMed: 14740189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 20083 EMBL AAO33821.1.
[2]	"Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis." Sprogoe D., van den Broek L.A., Mirza O., Kastrup J.S., Voragen A.G., Gajhede M., Skov L.K. Biochemistry 43:1156-1162(2004) [PubMed: 14756551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 20083 EMBL AAO33821.1.
[3]	Van den Broek L.A.M., Van Boxtel E.L., Beldman G., Voragen A.G.J. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 20083 EMBL AAO33821.1.
[4]	"Structural rearrangements of Sucrose Phosphorylase from Bifidobacterium adolescentis during sucrose conversion." Mirza O., Skov L.K., Sprogoe D., Van den Broek L.A.M., Voragen A.G.J., Katsrup J.S., Gajhede M. Submitted (MAR-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[5]	"Structural rearrangements of sucrose phosphorylase from Bifidobacterium adolescentis during sucrose conversion." Mirza O., Skov L.K., Sprogoe D., van den Broek L.A., Beldman G., Kastrup J.S., Gajhede M. J. Biol. Chem. 281:35576-35584(2006) [PubMed: 16990265] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH SUCROSE, SULFINATION AT CYS-356.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF543301 Genomic DNA. Translation: AAO33821.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R7A	X-ray	1.77	A/B	1-504	[»]
2GDU	X-ray	2.10	A/B	1-504	[»]
2GDV	X-ray	2.00	A/B	1-504	[»]

ProteinModelPortal

Q84HQ2.

SMR

Q84HQ2. Positions 1-504.

ModBase

Search...

Protein family/group databases

CAZy

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

PhylomeDB

Q84HQ2.

Enzyme and pathway databases

BRENDA

2.4.1.7. 1589.

Family and domain databases

InterPro

IPR015902. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR015325. Suc_Porlyase_C.
IPR016377. Sucrose_phosphorylase.
IPR022527. Sucrose_phosphorylase_GftA.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF09244. DUF1964. 1 hit.
[Graphical view]

PIRSF

PIRSF003059. Sucrose_phosphorylase. 1 hit.

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

TIGRFAMs

TIGR03852. Sucrose_gtfA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q84HQ2_BIFAD

Accession

Primary (citable) accession number: Q84HQ2

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	July 27, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)