Reviewed, UniProtKB/Swiss-Prot Q84HF6 (T23O_PSEFL)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:

kynA

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity. Required for synthesis of the siderophore quinolobactin.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Siderophore biosynthesis; quinolobactin biosynthesis. Ref.1 Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. Ref.1
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Tryptophan 2,3-dioxygenase		PRO_0000360126
Regions
	Region	28 – 32	5	Substrate binding By similarity
	Region	53 – 57	5	Substrate binding By similarity
Sites
	Metal binding	242	1	Iron (heme axial ligand) By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	126	1	Heme By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q84HF6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B3B526C3454FCF42	FASTA	284	33,036
	10 20 30 40 50 60 MCPCPYMHQA KPQQWHDAKL DFAESMSYGD YLGLDKVLDA QVPRSQQHNE MLFIIQHQAS 70 80 90 100 110 120 ELWMKLLLHE LRHARQLIDQ GQLAGSHRVL ARVLRIMEQM VSSWAILATL SPMEFISFRS 130 140 150 160 170 180 DLGNASGFQS YQYREIEFIF GNKNRAMLLP HQHTPQIAKN LEQCLHTPSL YDAIIQQMTR 190 200 210 220 230 240 QDLPICALRL DADPSEPTRS DASVEAAWVQ VYRQPERYWD LYQLGEKLMD IEDAFRQWRF 250 260 270 280 RHVTVVERVI GFKKGTGGTE GVEYLRKMLG TVLFPELWSL RSSL

References

[1]

"The Pseudomonas siderophore quinolobactin is synthesized from xanthurenic acid, an intermediate of the kynurenine pathway." Matthijs S., Baysse C., Koedam N., Tehrani K.A., Verheyden L., Budzikiewicz H., Schaefer M., Hoorelbeke B., Meyer J.-M., De Greve H., Cornelis P. Mol. Microbiol. 52:371-384(2004) [PubMed: 15066027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY271621 Genomic DNA. Translation: AAL65288.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_PSEFL

Accession

Primary (citable) accession number: Q84HF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)