Reviewed, UniProtKB/Swiss-Prot Q84HF5 (KMO_PSEFL)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
      Short name=PfKMO
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase

Gene names

Name:	kmo
Synonyms:	qbsG

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Probably required for the synthesis of quinolinic acid and the siderophore quinolobactin. Ref.1
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O. Ref.2
Cofactor	FAD. Ref.2
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3. Ref.1 Siderophore biosynthesis; quinolobactin biosynthesis. Ref.1
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
Biophysicochemical properties	Kinetic parameters: KM=11.8 µM for L-kynurenine (at 25 degrees Celsius) KM=8.5 µM for NADPH (at 25 degrees Celsius) KM=34.2 µM for oxygen (at 25 degrees Celsius) pH dependence: Optimum pH is 8.5.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Kynurenine 3-monooxygenase		PRO_0000361942

Sequences

	Sequence		Length	Mass (Da)
	Q84HF5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6515A55FFE8987F4	FASTA	461	50,712
	10 20 30 40 50 60 MTATDNARQV TIIGAGLAGT LVARLLARNG WQVNLFERRP DPRIETGARG RSINLALAER 70 80 90 100 110 120 GAHALRLAGL EREVLAEAVM MRGRMVHVPG TPPNLQPYGR DDSEVIWSIN RDRLNRILLD 130 140 150 160 170 180 GAEAAGASIH FNLGLDSVDF ARQRLTLSNV SGERLEKRFH LLIGADGCNS AVRQAMASVV 190 200 210 220 230 240 DLGEHLETQP HGYKELQITP EASAQFNLEP NALHIWPHGD YMCIALPNLD RSFTVTLFLH 250 260 270 280 290 300 HQSPAAQPAS PCFAQLVDGH AARRFFQRQF PDLSPMLDSL EQDFEHHPTG KLATLRLTTW 310 320 330 340 350 360 HVGGQAVLLG DAAHPMVPFH GQGMNCALED AVALAEHLQS AADNASALAA FTAQRQPDAL 370 380 390 400 410 420 AIQAMALENY VEMSSKVASP TYLLERELGQ IMAQRQPTRF IPRYSMVTFS RLPYAQAMAR 430 440 450 460 GQIQEQLLKF AVANHSDLTS INLDAVEHEV TRCLPPLSHL C

References

[1]

"The Pseudomonas siderophore quinolobactin is synthesized from xanthurenic acid, an intermediate of the kynurenine pathway." Matthijs S., Baysse C., Koedam N., Tehrani K.A., Verheyden L., Budzikiewicz H., Schaefer M., Hoorelbeke B., Meyer J.-M., De Greve H., Cornelis P. Mol. Microbiol. 52:371-384(2004) [PubMed: 15066027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE FUNCTION, PATHWAY. Strain: ATCC 17400.

[2]

"Heterologous expression and purification of kynurenine-3-monooxygenase from Pseudomonas fluorescens strain 17400." Crozier K.R., Moran G.R. Protein Expr. Purif. 51:324-333(2007) [PubMed: 16973376] [Abstract] Cited for: CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: 17400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY271621 Genomic DNA. Translation: AAL65289.1.
3D structure databases
HSSP	HSSP built from PDB template 1YKJ based on UniProtKB P20586.
ModBase	Search...
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_PSEFL

Accession

Primary (citable) accession number: Q84HF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)