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Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADUniRule annotation
Binding sitei83 – 831SubstrateUniRule annotation
Binding sitei89 – 891SubstrateUniRule annotation
Binding sitei96 – 961NADUniRule annotation
Binding sitei121 – 1211SubstrateUniRule annotation
Binding sitei152 – 1521SubstrateUniRule annotation
Active sitei176 – 1761Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADUniRule annotation
Nucleotide bindingi119 – 1213NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4226.
MEXT272630:GBY6-1477-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:MexAM1_META1p1537
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Malate dehydrogenasePRO_0000113457Add
BLAST

Proteomic databases

PRIDEiQ84FY8.

Structurei

3D structure databases

ProteinModelPortaliQ84FY8.
SMRiQ84FY8. Positions 1-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q84FY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSKIALIG AGQIGGTLAH LAGLKELGDV VLFDIVDGVP QGKALDIAES
60 70 80 90 100
APVDGFDAKY SGASDYSAIA GADVVIVTAG VPRKPGMSRD DLIGINLKVM
110 120 130 140 150
EAVGAGIKEH APDAFVICIT NPLDAMVWAL QKFSGLPTNK VVGMAGVLDS
160 170 180 190 200
ARFRHFLAEE FGVSVEDVTA FVLGGHGDDM VPLTRYSTVA GVPLTDLVKL
210 220 230 240 250
GWTTQEKLDA MVERTRKGGG EIVNLLKTGS AFYAPAASAI AMAESYLRDK
260 270 280 290 300
KRVLPCAAYL DGQYGIDGLY VGVPVVIGEN GVERVLEVTF NDDEKAMFEK
310 320
SVNSVKGLIE ACKSVNDKLA
Length:320
Mass (Da):33,647
Last modified:September 22, 2009 - v2
Checksum:iB2CF23AAA0F032EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091E → K in AAO24626 (PubMed:12730156).Curated
Sequence conflicti120 – 1201T → A in AAO24626 (PubMed:12730156).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181040 Genomic DNA. Translation: AAO24626.1.
CP001510 Genomic DNA. Translation: ACS39399.1.
RefSeqiWP_003599890.1. NC_012808.1.
YP_002962676.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39399; ACS39399; MexAM1_META1p1537.
KEGGimea:Mex_1p1537.
PATRICi22508920. VBIMetExt101010_1536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181040 Genomic DNA. Translation: AAO24626.1.
CP001510 Genomic DNA. Translation: ACS39399.1.
RefSeqiWP_003599890.1. NC_012808.1.
YP_002962676.1. NC_012808.1.

3D structure databases

ProteinModelPortaliQ84FY8.
SMRiQ84FY8. Positions 1-314.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ84FY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS39399; ACS39399; MexAM1_META1p1537.
KEGGimea:Mex_1p1537.
PATRICi22508920. VBIMetExt101010_1536.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4226.
MEXT272630:GBY6-1477-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view."
    Chistoserdova L., Chen S.-W., Lapidus A., Lidstrom M.E.
    J. Bacteriol. 185:2980-2987(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.

Entry informationi

Entry nameiMDH_METEA
AccessioniPrimary (citable) accession number: Q84FY8
Secondary accession number(s): C5B049
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: September 22, 2009
Last modified: May 27, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.