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Q84FY8

- MDH_METEA

UniProt

Q84FY8 - MDH_METEA

Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADUniRule annotation
    Binding sitei83 – 831SubstrateUniRule annotation
    Binding sitei89 – 891SubstrateUniRule annotation
    Binding sitei96 – 961NADUniRule annotation
    Binding sitei121 – 1211SubstrateUniRule annotation
    Binding sitei152 – 1521SubstrateUniRule annotation
    Active sitei176 – 1761Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADUniRule annotation
    Nucleotide bindingi119 – 1213NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-4226.
    MEXT272630:GBY6-1477-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:MexAM1_META1p1537
    OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
    Taxonomic identifieri272630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000009081: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Malate dehydrogenasePRO_0000113457Add
    BLAST

    Proteomic databases

    PRIDEiQ84FY8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84FY8.
    SMRiQ84FY8. Positions 1-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213794.
    KOiK00024.
    OMAiYAPSAFV.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q84FY8-1 [UniParc]FASTAAdd to Basket

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    MARSKIALIG AGQIGGTLAH LAGLKELGDV VLFDIVDGVP QGKALDIAES    50
    APVDGFDAKY SGASDYSAIA GADVVIVTAG VPRKPGMSRD DLIGINLKVM 100
    EAVGAGIKEH APDAFVICIT NPLDAMVWAL QKFSGLPTNK VVGMAGVLDS 150
    ARFRHFLAEE FGVSVEDVTA FVLGGHGDDM VPLTRYSTVA GVPLTDLVKL 200
    GWTTQEKLDA MVERTRKGGG EIVNLLKTGS AFYAPAASAI AMAESYLRDK 250
    KRVLPCAAYL DGQYGIDGLY VGVPVVIGEN GVERVLEVTF NDDEKAMFEK 300
    SVNSVKGLIE ACKSVNDKLA 320
    Length:320
    Mass (Da):33,647
    Last modified:September 22, 2009 - v2
    Checksum:iB2CF23AAA0F032EC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091E → K in AAO24626. (PubMed:12730156)Curated
    Sequence conflicti120 – 1201T → A in AAO24626. (PubMed:12730156)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY181040 Genomic DNA. Translation: AAO24626.1.
    CP001510 Genomic DNA. Translation: ACS39399.1.
    RefSeqiYP_002962676.1. NC_012808.1.

    Genome annotation databases

    EnsemblBacteriaiACS39399; ACS39399; MexAM1_META1p1537.
    GeneIDi7991516.
    KEGGimea:Mex_1p1537.
    PATRICi22508920. VBIMetExt101010_1536.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY181040 Genomic DNA. Translation: AAO24626.1 .
    CP001510 Genomic DNA. Translation: ACS39399.1 .
    RefSeqi YP_002962676.1. NC_012808.1.

    3D structure databases

    ProteinModelPortali Q84FY8.
    SMRi Q84FY8. Positions 1-314.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q84FY8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS39399 ; ACS39399 ; MexAM1_META1p1537 .
    GeneIDi 7991516.
    KEGGi mea:Mex_1p1537.
    PATRICi 22508920. VBIMetExt101010_1536.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213794.
    KOi K00024.
    OMAi YAPSAFV.
    OrthoDBi EOG6091FG.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-4226.
    MEXT272630:GBY6-1477-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view."
      Chistoserdova L., Chen S.-W., Lapidus A., Lidstrom M.E.
      J. Bacteriol. 185:2980-2987(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14718 / DSM 1338 / AM1.

    Entry informationi

    Entry nameiMDH_METEA
    AccessioniPrimary (citable) accession number: Q84FY8
    Secondary accession number(s): C5B049
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3