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Protein

Chorismate mutase AroH

Gene

aroH

Organism
Thermus thermophilus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.1 Publication

Catalytic activityi

Chorismate = prephenate.

Enzyme regulationi

Inhibited by 40% with 500 µM tyrosine, and a tyrosine concentration as high as 5 mM reduced activity to 5%.1 Publication

Kineticsi

  1. KM=290 µM for chorismate (at pH 7.6 and at 70 degrees Celsius)
  1. Vmax=198 µmol/min/mg enzyme (at pH 7.6 and at 70 degrees Celsius)

Temperature dependencei

Optimum temperature is 70 degrees Celsius.

Pathwayi: prephenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate.
Proteins known to be involved in this subpathway in this organism are:
  1. Chorismate mutase AroH (aroH)
This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei6Substrate1
Binding sitei89Substrate1
Binding sitei107Substrate1
Binding sitei115Substrate1

GO - Molecular functioni

  • chorismate mutase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BRENDAi5.4.99.5. 2305.
UniPathwayiUPA00120; UER00203.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate mutase AroH (EC:5.4.99.5)
Gene namesi
Name:aroH
Synonyms:aroG
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004149081 – 122Chorismate mutase AroHAdd BLAST122

Interactioni

Subunit structurei

Homotrimer.2 Publications

Protein-protein interaction databases

STRINGi262724.TTC0514.

Structurei

Secondary structure

1122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Beta strandi12 – 15Combined sources4
Helixi16 – 33Combined sources18
Helixi39 – 41Combined sources3
Beta strandi42 – 49Combined sources8
Helixi58 – 64Combined sources7
Helixi67 – 69Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi85 – 95Combined sources11
Helixi100 – 102Combined sources3
Beta strandi106 – 108Combined sources3
Helixi109 – 114Combined sources6
Helixi116 – 118Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ODEX-ray1.65A/B/C1-122[»]
1UFYX-ray0.96A1-122[»]
1UI9X-ray1.65A1-122[»]
ProteinModelPortaliQ84FH6.
SMRiQ84FH6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ84FH6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 120Chorismate mutase aroH-typePROSITE-ProRule annotationAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 77Substrate bindingBy similarityAdd BLAST31

Sequence similaritiesi

Contains 1 chorismate mutase aroH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105KHN. Bacteria.
COG4401. LUCA.

Family and domain databases

CDDicd02185. AroH. 1 hit.
Gene3Di3.30.1330.40. 1 hit.
InterProiIPR008243. Chorismate_mutase_AroH.
IPR013813. Endoribo_LPSP/chorism_mut-like.
[Graphical view]
PANTHERiPTHR21164. PTHR21164. 1 hit.
PfamiPF07736. CM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005965. Chor_mut_AroH. 1 hit.
ProDomiPD031888. Chorismate_mutase_AroH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55298. SSF55298. 1 hit.
TIGRFAMsiTIGR01796. CM_mono_aroH. 1 hit.
PROSITEiPS51167. CHORISMATE_MUT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84FH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRGIRGAIT VEEDTPEAIH QATRELLLKM LEANGIQSYE ELAAVIFTVT
60 70 80 90 100
EDLTSAFPAE AARQIGMHRV PLLSAREVPV PGSLPRVIRV LALWNTDTPQ
110 120
DRVRHVYLRE AVRLRPDLES AQ
Length:122
Mass (Da):13,650
Last modified:June 1, 2003 - v1
Checksum:i76BE0A500B3EE4E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY196198 Genomic DNA. Translation: AAO40746.1.

Genome annotation databases

PATRICi23951435. VBITheThe54392_0514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY196198 Genomic DNA. Translation: AAO40746.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ODEX-ray1.65A/B/C1-122[»]
1UFYX-ray0.96A1-122[»]
1UI9X-ray1.65A1-122[»]
ProteinModelPortaliQ84FH6.
SMRiQ84FH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262724.TTC0514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICi23951435. VBITheThe54392_0514.

Phylogenomic databases

eggNOGiENOG4105KHN. Bacteria.
COG4401. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00120; UER00203.
BRENDAi5.4.99.5. 2305.

Miscellaneous databases

EvolutionaryTraceiQ84FH6.

Family and domain databases

CDDicd02185. AroH. 1 hit.
Gene3Di3.30.1330.40. 1 hit.
InterProiIPR008243. Chorismate_mutase_AroH.
IPR013813. Endoribo_LPSP/chorism_mut-like.
[Graphical view]
PANTHERiPTHR21164. PTHR21164. 1 hit.
PfamiPF07736. CM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005965. Chor_mut_AroH. 1 hit.
ProDomiPD031888. Chorismate_mutase_AroH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55298. SSF55298. 1 hit.
TIGRFAMsiTIGR01796. CM_mono_aroH. 1 hit.
PROSITEiPS51167. CHORISMATE_MUT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROH_THETH
AccessioniPrimary (citable) accession number: Q84FH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.