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Protein

Chorismate mutase AroH

Gene

aroH

Organism
Thermus thermophilus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.1 Publication

Catalytic activityi

Chorismate = prephenate.

Enzyme regulationi

Inhibited by 40% with 500 µM tyrosine, and a tyrosine concentration as high as 5 mM reduced activity to 5%.1 Publication

Kineticsi

  1. KM=290 µM for chorismate (at pH 7.6 and at 70 degrees Celsius)
  1. Vmax=198 µmol/min/mg enzyme (at pH 7.6 and at 70 degrees Celsius)

Temperature dependencei

Optimum temperature is 70 degrees Celsius.

Pathwayi: prephenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate.
Proteins known to be involved in this subpathway in this organism are:
  1. Chorismate mutase AroH (aroH)
This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei6Substrate1
Binding sitei89Substrate1
Binding sitei107Substrate1
Binding sitei115Substrate1

GO - Molecular functioni

  • chorismate mutase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BRENDAi5.4.99.5 2305
UniPathwayiUPA00120; UER00203

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate mutase AroH (EC:5.4.99.5)
Gene namesi
Name:aroH
Synonyms:aroG
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid
DB02201 Malonate Ion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004149081 – 122Chorismate mutase AroHAdd BLAST122

Proteomic databases

PRIDEiQ84FH6

Interactioni

Subunit structurei

Homotrimer.2 Publications

Structurei

Secondary structure

1122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Beta strandi12 – 15Combined sources4
Helixi16 – 33Combined sources18
Helixi39 – 41Combined sources3
Beta strandi42 – 49Combined sources8
Helixi58 – 64Combined sources7
Helixi67 – 69Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi85 – 95Combined sources11
Helixi100 – 102Combined sources3
Beta strandi106 – 108Combined sources3
Helixi109 – 114Combined sources6
Helixi116 – 118Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ODEX-ray1.65A/B/C1-122[»]
1UFYX-ray0.96A1-122[»]
1UI9X-ray1.65A1-122[»]
ProteinModelPortaliQ84FH6
SMRiQ84FH6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ84FH6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 120Chorismate mutase aroH-typePROSITE-ProRule annotationAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 77Substrate bindingBy similarityAdd BLAST31

Phylogenomic databases

eggNOGiENOG4105KHN Bacteria
COG4401 LUCA

Family and domain databases

CDDicd02185 AroH, 1 hit
Gene3Di3.30.1330.40, 1 hit
InterProiView protein in InterPro
IPR008243 Chorismate_mutase_AroH
IPR035959 RutC-like_sf
PANTHERiPTHR21164 PTHR21164, 1 hit
PfamiView protein in Pfam
PF07736 CM_1, 1 hit
PIRSFiPIRSF005965 Chor_mut_AroH, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD031888 Chorismate_mutase_AroH, 1 hit
SUPFAMiSSF55298 SSF55298, 1 hit
TIGRFAMsiTIGR01796 CM_mono_aroH, 1 hit
PROSITEiView protein in PROSITE
PS51167 CHORISMATE_MUT_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q84FH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRGIRGAIT VEEDTPEAIH QATRELLLKM LEANGIQSYE ELAAVIFTVT
60 70 80 90 100
EDLTSAFPAE AARQIGMHRV PLLSAREVPV PGSLPRVIRV LALWNTDTPQ
110 120
DRVRHVYLRE AVRLRPDLES AQ
Length:122
Mass (Da):13,650
Last modified:June 1, 2003 - v1
Checksum:i76BE0A500B3EE4E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY196198 Genomic DNA Translation: AAO40746.1

Similar proteinsi

Entry informationi

Entry nameiAROH_THETH
AccessioniPrimary (citable) accession number: Q84FH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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