R-specific alcohol dehydrogenase - Lactobacillus brevis

Preferred order of sections

Names and origin

Protein names

Submitted name:
R-specific alcohol dehydrogenase EMBL CAD66648.1

Gene names

Name:

radh EMBL CAD66648.1

Organism

Lactobacillus brevis EMBL CAD66648.1

Taxonomic identifier

1580 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. RuleBase RU000363

Ontologies

Keywords
Ligand	NAD PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1 NADP PDB 1ZK4 Nucleotide-binding PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1 PDB 1ZK4
Molecular function	Oxidoreductase RuleBase RU004267
Technical term	3D-structure PDB 1ZJY PDB 1ZK0 PDB 1NXQ PDB 1ZJZ PDB 1ZK1 PDB 1ZK4 PDB 1ZK3 PDB 1ZK2
Gene Ontology (GO)
Molecular function	nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

14 – 20

7

NAD PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1

Nucleotide binding

14 – 19

6

NADP PDB 1ZK4

Nucleotide binding

37 – 39

3

NADP PDB 1ZK4

Nucleotide binding

63 – 64

2

NAD PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1

Nucleotide binding

63 – 64

2

NADP PDB 1ZK4

Nucleotide binding

90 – 92

3

NADP PDB 1ZK4

Sites

Binding site

90

1

NAD PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1

Binding site

156

1

NAD PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1

Binding site

156

1

NADP PDB 1ZK4

Binding site

160

1

NAD PDB 1ZK1

Binding site

191

1

NAD; via amide nitrogen and carbonyl oxygen PDB 1ZJY PDB 1ZK0 PDB 1ZJZ PDB 1ZK1

Binding site

191

1

NADP; via carbonyl oxygen PDB 1ZK4

Sequences

Sequence

Length

Mass (Da)

Tools

Q84EX5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: CD9356D896011C74
Show »

FASTA

252

26,758

        10         20         30         40         50         60 
MSNRLDGKVA IITGGTLGIG LAIATKFVEE GAKVMITGRH SDVGEKAAKS VGTPDQIQFF 

        70         80         90        100        110        120 
QHDSSDEDGW TKLFDATEKA FGPVSTLVNN AGIAVNKSVE ETTTAEWRKL LAVNLDGVFF 

       130        140        150        160        170        180 
GTRLGIQRMK NKGLGASIIN MSSIEGFVGD PSLGAYNASK GAVRIMSKSA ALDCALKDYD 

       190        200        210        220        230        240 
VRVNTVHPGY IKTPLVDDLP GAEEAMSQRT KTPMGHIGEP NDIAYICVYL ASNESKFATG 

       250 
SEFVVDGGYT AQ

« Hide

References

[1]	"Biochemical and Molecular Biological Characterization of Novel Microbial NAD(P)-dependent Alcohol Dehydrogenases." Riebel-Bommarius B.R. Thesis (1997), Department of Institute of Enzyme Technology, University of Duesseldorf, Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 20054 EMBL CAD66648.1.
[2]	Riebel-Bommarius B.R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 20054 EMBL CAD66648.1.
[3]	"The crystal structure of R-specific alcohol dehydrogenase from Lactobacillus brevis suggests the structural basis of its metal dependency." Niefind K., Muller J., Riebel B., Hummel W., Schomburg D. J. Mol. Biol. 327:317-328(2003) [PubMed: 12628239] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) OF 2-252.
[4]	"Atomic resolution structures of R-specific alcohol dehydrogenase from Lactobacillus brevis provide the structural bases of its substrate and cosubstrate specificity." Schlieben N.H., Niefind K., Muller J., Riebel B., Hummel W., Schomburg D. J. Mol. Biol. 349:801-813(2005) [PubMed: 15896805] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.00 ANGSTROMS) OF 2-252 IN COMPLEX WITH NAD AND NADP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ544275 Genomic DNA. Translation: CAD66648.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NXQ	X-ray	1.79	A	2-252	[»]
1ZJY	X-ray	1.05	A	2-252	[»]
1ZJZ	X-ray	1.10	A	2-252	[»]
1ZK0	X-ray	1.55	A	2-252	[»]
1ZK1	X-ray	1.78	A	2-252	[»]
1ZK2	X-ray	1.55	A	2-252	[»]
1ZK3	X-ray	2.20	A/B/C/D/E/F/G/H	2-252	[»]
1ZK4	X-ray	1.00	A	2-252	[»]

ProteinModelPortal

Q84EX5.

SMR

Q84EX5. Positions 2-252.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q84EX5_LACBR

Accession

Primary (citable) accession number: Q84EX5

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	July 27, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)