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Q84C00

- GUB_CLOTM

UniProt

Q84C00 - GUB_CLOTM

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Protein
Beta-glucanase
Gene
licB, lam1
Organism
Clostridium thermocellum
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei136 – 1361Nucleophile By similarity
Active sitei140 – 1401Proton donor By similarity

GO - Molecular functioni

  1. licheninase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Laminarinase
Lichenase
Gene namesi
Name:licB
Synonyms:lam1
OrganismiClostridium thermocellum
Taxonomic identifieri1515 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Chaini28 – 334307Beta-glucanase
PRO_0000011792Add
BLAST

Interactioni

Subunit structurei

May form part of a multienzyme complex (cellulosome).

Structurei

3D structure databases

ProteinModelPortaliQ84C00.
SMRiQ84C00. Positions 33-246.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati273 – 296241
Add
BLAST
Repeati308 – 331242
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 331592 X 24 AA approximate repeats
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi252 – 26918Pro/Thr-rich (linker)
Add
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84C00-1 [UniParc]FASTAAdd to Basket

« Hide

MKNRVISLLM ASLLLVLSVI VAPFYKAEAA TVVNTPFVAV FSNFDSSQWE    50
KADWANGSVF NCVWKPSQVT FSNGKMILTL DREYGGSYPY KSGEYRSKSF 100
FGYGYYEVRM KAAKNVGIVS SFFTYTGPSD NNPWDEIDIE FLGKDTTKAQ 150
FNWYKNEVGG NEYLHNLGFD ASQDFHTYGF EWRPDYIDFY VDGKKVYRGT 200
RNIPVTPGKI MMNLWPGKGV DEWLGRYDGR TPLQAEYEYV KYYPNGVPQD 250
NPTPTPTIAP STPTNPNLPL KGDVNGDGHV NSSDYSLFKR YLLRVIDRFP 300
VGDQSVADVN RDGRIDSTDL TMLKRYLIRA IPSL 334
Length:334
Mass (Da):37,942
Last modified:June 1, 2003 - v1
Checksum:i3079DEF1CEE5A664
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971S → T in CAA41281. 1 Publication
Sequence conflicti97 – 971S → T1 Publication
Sequence conflicti149 – 1491A → V in CAA41281. 1 Publication
Sequence conflicti149 – 1491A → V1 Publication
Sequence conflicti157 – 1571E → G in CAA41281. 1 Publication
Sequence conflicti157 – 1571E → G1 Publication
Sequence conflicti218 – 2181K → I in CAA41281. 1 Publication
Sequence conflicti218 – 2181K → I1 Publication
Sequence conflicti304 – 33431QSVAD…AIPSL → PQDGCGRHDRVVDSGSK in CAA41281. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58392 Genomic DNA. Translation: CAA41281.1.
AY225318 Genomic DNA. Translation: AAO74890.1.
PIRiJS0611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58392 Genomic DNA. Translation: CAA41281.1 .
AY225318 Genomic DNA. Translation: AAO74890.1 .
PIRi JS0611.

3D structure databases

ProteinModelPortali Q84C00.
SMRi Q84C00. Positions 33-246.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR008264. Beta_glucanase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view ]
Pfami PF00404. Dockerin_1. 2 hits.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PRINTSi PR00737. GLHYDRLASE16.
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Clostridium thermocellum laminarinase gene."
    Zverlov V.V., Laptev D.A., Tishkov V.I., Velikodvorskaja G.A.
    Biochem. Biophys. Res. Commun. 181:507-512(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: F7.
  2. Zverlov V.V.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Lv W.-P., Xu Z.-R., Li W.-F., Sun J.-Y., Xu Y.-X., Gu S.-H.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning the Clostridium thermocellum thermostable laminarinase gene in Escherichia coli; the properties of the enzyme thus produced."
    Zverlov V.V., Velikodvorskaja G.A.
    Biotechnol. Lett. 12:811-816(1990)
    Cited for: CHARACTERIZATION.
    Strain: F7.

Entry informationi

Entry nameiGUB_CLOTM
AccessioniPrimary (citable) accession number: Q84C00
Secondary accession number(s): P29716, P37074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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