Methionine gamma-lyase - Citrobacter freundii

Preferred order of sections

Names and origin

Protein names

Submitted name:
Methionine gamma-lyase EMBL AAO46884.1

Gene names

Name:

megL EMBL AAO46884.1

Organism

Citrobacter freundii EMBL AAO46884.1

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the trans-sulfuration enzymes family. RuleBase RU004079

Ontologies

Keywords
Molecular function	Lyase EMBL AAO46884.1
Technical term	3D-structure PDB 2RFV PDB 3JWA PDB 3JWB PDB 3MKJ PDB 1Y4I PDB 3JW9
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Amino acid modifications

Modified residue

4

1

Cysteine sulfenic acid (-SOH) PDB 2RFV PDB 3JWA PDB 3JWB PDB 3MKJ

Modified residue

115

1

Cysteine sulfenic acid (-SOH) PDB 3MKJ

Modified residue

193

1

Cysteine sulfenic acid (-SOH) PDB 3MKJ

Modified residue

245

1

Cysteine sulfenic acid (-SOH) PDB 3MKJ

Sequences

Sequence

Length

Mass (Da)

Tools

Q84AR1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 915176498E76A125
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FASTA

398

42,915

        10         20         30         40         50         60 
MSDCRTYGFN TQIVHAGQQP DPSTGALSTP IFQTSTFVFD SAEQGAARFA LEESGYIYTR 

        70         80         90        100        110        120 
LGNPTTDALE KKLAVLERGE AGLATASGIS AITTTLLTLC QQGDHIVSAS AIYGCTHAFL 

       130        140        150        160        170        180 
SHSMPKFGIN VRLVDAGKPE EIRAAMRPET KVVYIETPAN PTLSLVDIET VAGIAHQQGA 

       190        200        210        220        230        240 
LLVVDNTFMS PYCQQPLQLG ADIVVHSVTK YINGHGDVIG GIIVGKQEFI DQARFVGLKD 

       250        260        270        280        290        300 
ITGGCMSPFN AWLTLRGVKT LGIRMERHCE NALKIARFLE GHPSITRVYY PGLSSHPQYE 

       310        320        330        340        350        360 
LGQRQMSLPG GIISFEIAGG LEAGRRMINS VELCLLAVSL GDTETLIQHP ASMTHSPVAP 

       370        380        390 
EERLKAGITD GLIRLSVGLE DPEDIINDLE HAIRKATF

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References

[1]	"A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase." Manukhov I.V., Mamaeva D.V., Rastorguev S.M., Faleev N.G., Morozova E.A., Demidkina T.V., Zavilgelsky G.B. J. Bacteriol. 187:3889-3893(2005) [PubMed: 15901718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structure of Citrobacter freundii L-methionine gamma-lyase." Mamaeva D.V., Morozova E.A., Nikulin A.D., Revtovich S.V., Nikonov S.V., Garber M.B., Demidkina T.V. Acta Crystallogr. F 61:546-549(2005) [PubMed: 16511092] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).
[3]	"Structures of methionine gamma-lyase from Citrobacter freundii with non-covalently bound ligands." Revtovich S.V., Nikulin A.D., Morozova E.A., Garber M.B., Demidkina T.V. Submitted (SEP-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
[4]	"Exploring methionine gamma-lyase structure-function relationship via microspectrophotometry and X-ray crystallography." Ronda L., Bazhulina N.P., Morozova E.A., Revtovich S.V., Chekhov V.O., Nikulin A.D., Demidkina T.V., Mozzarelli A. Biochim. Biophys. Acta 0:0-0(2010) [PubMed: 20601224] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS), CYSTEINE SULFENIC ACID (-SOH) AT CYS-4; CYS-115; CYS-193 AND CYS-245.
[5]	"High-resolution structure of methionine gamma-lyase from Citrobacter freundii." Nikulin A., Revtovich S., Morozova E., Nevskaya N., Nikonov S., Garber M., Demidkina T. Acta Crystallogr. D Biol. Crystallogr. 64:211-218(2008) [PubMed: 18219122] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS), CYSTEINE SULFENIC ACID (-SOH) AT CYS-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY204910 Genomic DNA. Translation: AAO46884.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y4I	X-ray	1.90	A	1-398	[»]
2RFV	X-ray	1.36	A	1-398	[»]
3JW9	X-ray	1.80	A	1-398	[»]
3JWA	X-ray	1.45	A	1-398	[»]
3JWB	X-ray	1.63	A	1-398	[»]
3MKJ	X-ray	1.65	A	1-398	[»]

ProteinModelPortal

Q84AR1.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

4.4.1.11. 2347.

Family and domain databases

InterPro

IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006237. Met_gamma_lys.
IPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]

Gene3D

G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.

PANTHER

PTHR11808. Cys_Met_Meta_PP. 1 hit.

Pfam

PF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]

PIRSF

PIRSF001434. CGS. 1 hit.

SUPFAM

SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.

TIGRFAMs

TIGR01328. Met_gam_lyase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q84AR1_CITFR

Accession

Primary (citable) accession number: Q84AR1

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	July 27, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)