SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q84940

- NSP1_ROTP5

UniProt

Q84940 - NSP1_ROTP5

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Non-structural protein 1
Gene
N/A
Organism
Rotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Taxonomic identifieri10915 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Non-structural protein 1
PRO_0000367822Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Protein-protein interaction databases

IntActiQ84940. 19 interactions.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8181RNA-binding By similarity
Add
BLAST
Regioni82 – 17695Important for cytoskeleton localization
Add
BLAST
Regioni313 – 486174Interaction with IRF3
Add
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84940-1 [UniParc]FASTAAdd to Basket

« Hide

MATFKDACYY YKRINKLNHA VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD    50
LTYCRGCTMY HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLMNLVD 100
MYDTLFPMNQ KIVDKFINNT RQHKCRNECV NQWYNHLLMP ITLQSLSIEL 150
DGDVYYIFGY YDDMNNVNQT PFSFVNLVDI YDKLLLDDVN FTRMSFLPVT 200
LQQEYALRYF SKSRFISEQR KCVSDSHFSI NVLENLHNPS FKMQITRNCS 250
ELSSDWNGAC KLVKDTSAYF NILKTSHVEF YSISTRCRVF TQRKLKIASK 300
LIKPNYITSN HRTSATEVHN CKWCSINSSY TVWNDFRVKK IYDNIFNFLR 350
ALVKSNVNVG HCSSQEKIYE CVENILDVCD NEKWKTSVTK IFNYLEPVEL 400
NAVNYVLFNH EVNWDVINVL VQSIGKVPQI LTLNDVTTIM QSIIYEWFDT 450
KYMRNTPMTT FTVDKLRRLC TGSKTVDYDS GISDVE 486
Length:486
Mass (Da):57,235
Last modified:November 1, 1996 - v1
Checksum:iE42BAC8CDF5BF666
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021Y → F in CAA78092. 1 Publication
Sequence conflicti227 – 2271H → R in CAA78092. 1 Publication
Sequence conflicti250 – 2501S → N in CAA78092. 1 Publication
Sequence conflicti321 – 3211C → S in CAA78092. 1 Publication
Sequence conflicti370 – 3701E → Q in CAA78092. 1 Publication
Sequence conflicti449 – 4502DT → TR in CAA78092. 1 Publication
Sequence conflicti478 – 4792YD → CN in CAA78092. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08432 Genomic RNA. Translation: AAA50495.1.
Z12107 Genomic RNA. Translation: CAA78092.1.
D38153 Genomic RNA. Translation: BAA20544.1.
PIRiS31805.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08432 Genomic RNA. Translation: AAA50495.1 .
Z12107 Genomic RNA. Translation: CAA78092.1 .
D38153 Genomic RNA. Translation: BAA20544.1 .
PIRi S31805.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

IntActi Q84940. 19 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002148. Rotavirus_NSP1.
[Graphical view ]
Pfami PF00981. Rota_NS53. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
    Dunn S.J., Cross T.L., Greenberg H.B.
    Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Molecular biology of rotaviruses. IX. Conservation and divergence in genome segment 5."
    Xu L.I., Tian Y., Tarlow O., Harbour D.A., McCrae M.A.
    J. Gen. Virol. 75:3413-3421(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains."
    Kojima K., Taniguchi K., Kobayashi N.
    Arch. Virol. 141:1-12(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
    Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
    J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], FUNCTION.

Entry informationi

Entry nameiNSP1_ROTP5
AccessioniPrimary (citable) accession number: Q84940
Secondary accession number(s): Q85037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

NSP1 has been shown (1 Publication) to be almost unable to bind and degrade IRF3 in cell culture. However, this does not implies that it is the case in vivo, since the down-regulation of host antiviral state is not essential for the virus in cell culture and the used cells were not of porcine origin.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi