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Q84940 (NSP1_ROTP5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Taxonomic identifier10915 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity. Ref.4

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Caution

NSP1 has been shown (Ref.4) to be almost unable to bind and degrade IRF3 in cell culture. However, this does not implies that it is the case in vivo, since the down-regulation of host antiviral state is not essential for the virus in cell culture and the used cells were not of porcine origin.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Non-structural protein 1
PRO_0000367822

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17695Important for cytoskeleton localization
Region313 – 486174Interaction with IRF3

Experimental info

Sequence conflict1021Y → F in CAA78092. Ref.2
Sequence conflict2271H → R in CAA78092. Ref.2
Sequence conflict2501S → N in CAA78092. Ref.2
Sequence conflict3211C → S in CAA78092. Ref.2
Sequence conflict3701E → Q in CAA78092. Ref.2
Sequence conflict449 – 4502DT → TR in CAA78092. Ref.2
Sequence conflict478 – 4792YD → CN in CAA78092. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q84940 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E42BAC8CDF5BF666

FASTA48657,235
        10         20         30         40         50         60 
MATFKDACYY YKRINKLNHA VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD LTYCRGCTMY 

        70         80         90        100        110        120 
HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLMNLVD MYDTLFPMNQ KIVDKFINNT 

       130        140        150        160        170        180 
RQHKCRNECV NQWYNHLLMP ITLQSLSIEL DGDVYYIFGY YDDMNNVNQT PFSFVNLVDI 

       190        200        210        220        230        240 
YDKLLLDDVN FTRMSFLPVT LQQEYALRYF SKSRFISEQR KCVSDSHFSI NVLENLHNPS 

       250        260        270        280        290        300 
FKMQITRNCS ELSSDWNGAC KLVKDTSAYF NILKTSHVEF YSISTRCRVF TQRKLKIASK 

       310        320        330        340        350        360 
LIKPNYITSN HRTSATEVHN CKWCSINSSY TVWNDFRVKK IYDNIFNFLR ALVKSNVNVG 

       370        380        390        400        410        420 
HCSSQEKIYE CVENILDVCD NEKWKTSVTK IFNYLEPVEL NAVNYVLFNH EVNWDVINVL 

       430        440        450        460        470        480 
VQSIGKVPQI LTLNDVTTIM QSIIYEWFDT KYMRNTPMTT FTVDKLRRLC TGSKTVDYDS 


GISDVE 

« Hide

References

[1]"Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
Dunn S.J., Cross T.L., Greenberg H.B.
Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Molecular biology of rotaviruses. IX. Conservation and divergence in genome segment 5."
Xu L.I., Tian Y., Tarlow O., Harbour D.A., McCrae M.A.
J. Gen. Virol. 75:3413-3421(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains."
Kojima K., Taniguchi K., Kobayashi N.
Arch. Virol. 141:1-12(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[4]"Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U08432 Genomic RNA. Translation: AAA50495.1.
Z12107 Genomic RNA. Translation: CAA78092.1.
D38153 Genomic RNA. Translation: BAA20544.1.
PIRS31805.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ84940. 19 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTP5
AccessionPrimary (citable) accession number: Q84940
Secondary accession number(s): Q85037
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families