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Protein

Genome polyprotein

Gene
N/A
Organism
Plum pox potyvirus (strain SK 68) (PPV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Capsid protein: involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
Nuclear inclusion protein B: an RNA-dependent RNA polymerase that plays an essential role in the virus replication.
Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity (By similarity).By similarity
Cytoplasmic inclusion protein: has helicase activity. It may be involved in replication.
Both 6K peptides are indispensable for virus replication.By similarity
Nuclear inclusion protein A: has RNA-binding and proteolytic activities.

Catalytic activityi

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei216For P1 proteinase activityBy similarity1
Active sitei225For P1 proteinase activitySequence analysis1
Active sitei259For P1 proteinase activityBy similarity1
Active sitei652For helper component proteinase activityPROSITE-ProRule annotation1
Active sitei725For helper component proteinase activityPROSITE-ProRule annotation1
Active sitei2095For nuclear inclusion protein A activityPROSITE-ProRule annotation1
Active sitei2130For nuclear inclusion protein A activityPROSITE-ProRule annotation1
Active sitei2200For nuclear inclusion protein A activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1253 – 1260ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
N-terminal protein
Helper component proteinase (EC:3.4.22.45)
Short name:
HC-pro
6 kDa protein 1
Short name:
6K1
6 kDa protein 2
Short name:
6K2
Alternative name(s):
VPg
Nuclear inclusion protein A (EC:3.4.22.44)
Short name:
NI-a
Short name:
NIa
Alternative name(s):
49 kDa proteinase
Short name:
49 kDa-Pro
NIa-pro
Nuclear inclusion protein B (EC:2.7.7.48)
Short name:
NI-b
Short name:
NIb
Alternative name(s):
RNA-directed RNA polymerase
Capsid protein
Short name:
CP
Alternative name(s):
Coat protein
OrganismiPlum pox potyvirus (strain SK 68) (PPV)
Taxonomic identifieri103927 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stagePotyviridaePotyvirus
Virus hostiPrunus armeniaca (Apricot) (Armeniaca vulgaris) [TaxID: 36596]
Prunus cerasifera (cherry plum) [TaxID: 36595]
Prunus domestica (Garden plum) [TaxID: 3758]
Prunus glandulosa [TaxID: 105665]
Prunus persica (Peach) (Amygdalus persica) [TaxID: 3760]
Prunus salicina [TaxID: 88123]
Prunus spinosa (Blackthorn) (Prunus domestica var. spinosa) [TaxID: 114937]
Proteomesi
  • UP000007637 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004200091 – 3140Genome polyproteinAdd BLAST3140
ChainiPRO_00000403511 – 308P1 proteinaseSequence analysisAdd BLAST308
ChainiPRO_0000040352309 – 766Helper component proteinaseSequence analysisAdd BLAST458
ChainiPRO_0000040353767 – 1116Protein P3By similarityAdd BLAST350
ChainiPRO_00000403541117 – 11686 kDa protein 1By similarityAdd BLAST52
ChainiPRO_00000403551169 – 1803Cytoplasmic inclusion proteinBy similarityAdd BLAST635
ChainiPRO_00000403561804 – 18566 kDa protein 2By similarityAdd BLAST53
ChainiPRO_00000403571857 – 2049Viral genome-linked proteinBy similarityAdd BLAST193
ChainiPRO_00000403582050 – 2292Nuclear inclusion protein ABy similarityAdd BLAST243
ChainiPRO_00000403592293 – 2810Nuclear inclusion protein BBy similarityAdd BLAST518
ChainiPRO_00000403602811 – 3140Capsid proteinBy similarityAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1919O-(5'-phospho-RNA)-tyrosineBy similarity1

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
Genome polyprotein of potyviruses undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei308 – 309Cleavage; by P1 proteinaseSequence analysis2
Sitei766 – 767Cleavage; by autolysisPROSITE-ProRule annotation2
Sitei1116 – 1117Cleavage; by NIa-proBy similarity2
Sitei1168 – 1169Cleavage; by NIa-proBy similarity2
Sitei1803 – 1804Cleavage; by NIa-proBy similarity2
Sitei1856 – 1857Cleavage; by NIa-proBy similarity2
Sitei2049 – 2050Cleavage; by NIa-proBy similarity2
Sitei2292 – 2293Cleavage; by NIa-proBy similarity2
Sitei2810 – 2811Cleavage; by NIa-proBy similarity2

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ84934.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini644 – 766Peptidase C6PROSITE-ProRule annotationAdd BLAST123
Domaini1240 – 1392Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST153
Domaini1411 – 1570Helicase C-terminalPROSITE-ProRule annotationAdd BLAST160
Domaini2050 – 2268Peptidase C4PROSITE-ProRule annotationAdd BLAST219
Domaini2534 – 2658RdRp catalyticPROSITE-ProRule annotationAdd BLAST125

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi360 – 363Involved in interaction with stylet and aphid transmissionBy similarity4
Motifi618 – 620Involved in virions binding and aphid transmissionBy similarity3
Motifi1342 – 1345DECH box4
Motifi1897 – 1904Nuclear localization signalSequence analysis8

Domaini

The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 peptidase C4 domain.PROSITE-ProRule annotation
Contains 1 peptidase C6 domain.PROSITE-ProRule annotationCurated
Contains 1 peptidase S30 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011492. DEAD_Flavivir.
IPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002540. Pept_S30_P1_potyvir.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF07652. Flavi_DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF01577. Peptidase_S30. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Genome polyprotein (identifier: Q84934-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTIVFGSFT CHLDAAIHQD NADRLAKAWT RPENRQVSNV HLLCRRAAKS
60 70 80 90 100
LINTYESATA SAWKGLEEKL QPMFAKREFS KTVTKRKGLR CFKESSEKFI
110 120 130 140 150
EKKLKKQYKE ERERFQFLNG PDAIVNQISV DKCEASVWVP FPHIIEKPSF
160 170 180 190 200
TTPSMKKKVV FTKVRMSEAS LQLFMRRVAA NAKANGQKVE IIGRKRVVGH
210 220 230 240 250
YTTKSRLTYF RTHVRHLDGS KPRYDLVLDE ATKKILQLFA NTSGFHHVHK
260 270 280 290 300
KGEITPGMSG FVVNPMNLSD PMHVYDTDLF IVRGKHNSIL VDSRCKVSKE
310 320 330 340 350
QSNEIVHYSD PGKQFWDGFT NSFMQCKLRE TDHQCTSDLD VKECGYVAAL
360 370 380 390 400
VCQAIIPCGK ITCLQCAQKY SYMSQQEIRD RFSTVIEQHE KTVMDNYPQF
410 420 430 440 450
SHVLAFLKRY RELMRVENQN YEAFKDITHM IGERKEAPFS HLNKINELII
460 470 480 490 500
KGGMMSAQDY IEASDHLREL ARYQKNRTEN IRSGSIKAFR NKISSKAHVN
510 520 530 540 550
MQLMCDNQLD TNGNFVWGQR EYHAKRFFRN YFDVIDVSEG YRRHIVRENP
560 570 580 590 600
RGIRKLAIGN LVMSTNLAAL RKQLLGEECI HFEVSKECTS KRGENFVYQC
610 620 630 640 650
CCVTHEDGTP LESEIISPTK NHLVVGNSGD SKYVDLPTAK GGAMFIAKAG
660 670 680 690 700
YCYINIFLAM LININEDEAK SFTKTVRDTI VPKLGTWPSM MDLATACHFL
710 720 730 740 750
AVLYPETRNA ELPRILVDHE AKIFHVVDSF GSLSTGMHVL KANTINQLIS
760 770 780 790 800
FASDTLDSSM KTYLVGGLEV DKCDEFKNVK LLIRSIYKPQ IMEQVLKEEP
810 820 830 840 850
YLLLMSVLSP GVLMALFNSG SLEKATQYWI ARSHSLAAIT AMLSALAAKV
860 870 880 890 900
SLASTLNAQM SVIDEHAAVL CDSVFVGTKP YASYMMAVKT LERMKARTES
910 920 930 940 950
DHTLNDLGFS VLRQATPHLV EKSYLQELEQ AWRELSWSER FSAILESQRW
960 970 980 990 1000
RKHIPKPFIP KDAADLGGRY DISVRSLLGS QYKRLKDVVR RKRDDVVCYT
1010 1020 1030 1040 1050
HQSMGKLFCK AIGISTSFLP STLKMFDMLI IFGLLLSIGA TCNSMINEHK
1060 1070 1080 1090 1100
HLKQVAADRE DKKRFKRLQV LYTRLLEKIG CTPTADEFLE YVQGENPDLS
1110 1120 1130 1140 1150
KYAEDLIGDG QVVVHQSKRD SQANLERVAA FVALVMMLFD SERSDGVYKI
1160 1170 1180 1190 1200
LNKLKGVMGS IDQTVHHQNL DDIEDMLDEK KLTVDFVLQS NEVAPTVPFD
1210 1220 1230 1240 1250
STFEKWWTNQ LETGNVIPHY RTEGHFLEFT RENAAHIANE VMHGSHQDIL
1260 1270 1280 1290 1300
IRGAVGSGKS TGLPFHLSKK GHVLLIEPTR PLAENVCKQL RGQPFNVNPT
1310 1320 1330 1340 1350
LRMRGMSTFG STPITVMTSG YALHFLANNP TYLDNYKCII FDECHVHDAS
1360 1370 1380 1390 1400
AMAFRCLLSE YSYPGKILKV SATPPGYEVD FKTQKEVKVI VEEALSFQQF
1410 1420 1430 1440 1450
VSNLGTGCNS DILKHGVNVL VYVASYNEVD TLSKLLTDRS FKVSKVDGRT
1460 1470 1480 1490 1500
MKVGNVEIPT SGTQAKPHFV VATNIIENGV TLDIDVVVDF GLKVVPILDI
1510 1520 1530 1540 1550
DNRLVRYTKK SISYGERIQR LGRVGRNKPG MALRIGFTEK GLTQIPPIIA
1560 1570 1580 1590 1600
TEAAFLCFTY GLPVMTNGVS TSLLAMCTVK QARTMQQFEL SPFYTVALVR
1610 1620 1630 1640 1650
FDGTMHQEIF RLLKSYRLRD SEVILNKLAI PNSNVCGWMS VRDYKRQGCN
1660 1670 1680 1690 1700
LDLDENIRVP FYVKDIPETL HDKVWQAVET HKSDAGFGRI CSSSACKIAY
1710 1720 1730 1740 1750
TLQTDIHSIP RTVKIIDALL EQERTKQAHF RAMTSQSCSS SNFSLSSITS
1760 1770 1780 1790 1800
AIRSKYAKDH TEENIGVLQM AKSQLLEFKN LNIDPSYPEL VRNFGALECV
1810 1820 1830 1840 1850
HHQTKEGVSK TLQLKGHWNK RLITRDATLM LGVLGGGAWM IFTYLKDSFQ
1860 1870 1880 1890 1900
EEVVHQGFNR RQRQKLKFRQ ARDNRMAREV YGDDSTMEDY FGSAYSKKGK
1910 1920 1930 1940 1950
SKGKTRGMGT KTRKFVNMYG YDPTDYNFVR FVDPLTGHTL DENPLMDINL
1960 1970 1980 1990 2000
VQEHFSQIRN DYIGDDKITM QHIMSNPGIV AYYIKDATQK ALKVDLTPHN
2010 2020 2030 2040 2050
PLRVCDKTAT IAGFPEREFE LRQTGHPTFV EPNAIPKINE VGQEEVDHES
2060 2070 2080 2090 2100
KSLFRGLRDY NPIASSICQL NNSSGTRHSE MFGLGFGGLI VTNQHLFKRN
2110 2120 2130 2140 2150
DGELTIRSHH GEFVVKDTKT LKLLPCKGRD ILIIRLPKDF PPFPKRLQFR
2160 2170 2180 2190 2200
TPTTEDRVCL IGSNFQTKSI SSTMSETSAT YSVDNSHFWK HWISTKDGHC
2210 2220 2230 2240 2250
GLPIVSTRDG SILGLHSLAN STNTQNFYAA FPDNFETTYL SNQDNDNWIK
2260 2270 2280 2290 2300
QWRYNPDEVC WGSLELKRDI PQMPFTVCKL LTDLDREFVY NQSKTTHWLR
2310 2320 2330 2340 2350
DKLEGNLKAV GACPGQLVTK HVVKGKCTLF ETYLLTHPEE HEFFRPLMGA
2360 2370 2380 2390 2400
YQKSALNKDE YVKDLMKYSK PIVVGAVDCE QFERALDVVI SMLISKGFEE
2410 2420 2430 2440 2450
CNYVTDPDDI FSALNMKAAV GALYSGKKRD YFKNASEQDK EDFIKASCKR
2460 2470 2480 2490 2500
LFMGKKGVWN GSLKAELRPK EKVEANKTRS FTAAPIDTLL GGKVCVDDFN
2510 2520 2530 2540 2550
NQFYSLNLHC PWSVGMTKFR GGWDKLLRAL PDGWIYCDAD GSQFDSSLSP
2560 2570 2580 2590 2600
YLINAVLNIR LAFMEEWDIG EQMLSNLYTE IVYTPIATPD GTIVKKFKGN
2610 2620 2630 2640 2650
NSGQPSTVVD NTLMVILAMT YSLLKLGYHP DTHECICRYF VNGDDLVLAV
2660 2670 2680 2690 2700
HPAYESMYDE LQEHFSQLGL NYTFTTKTEN KEELWFMSHR GVLFEDMYIP
2710 2720 2730 2740 2750
KLEPERIVSI LEWDRSNEPI HRLEAICASM VEAWGYKELL REIRKFYSWV
2760 2770 2780 2790 2800
LEQAPYNALS KDGKAPYIAE TALRKLYTDS EASETEIERY LEAFYNDVDD
2810 2820 2830 2840 2850
SLDSNIVIHQ ADEEEDDEEV DAGRPTVVTA PAATVATTQP APVIQPAPQT
2860 2870 2880 2890 2900
TAPMFNPIFT PATTQPAVRP VPPISGAKPR SFGVYGNEDA SPSTSNTLVN
2910 2920 2930 2940 2950
TGRDRDVDAG SIGTFAVPRL KTMTSKLSLP KVKGKAIMNL NHLAHYSPAQ
2960 2970 2980 2990 3000
VDLSNTRAPQ SCFQTWYEGV KRDYDVTDEE MSIILNGLMV WCIENGTSPN
3010 3020 3030 3040 3050
INGMWVMMDG ETQVEYPIKP LLDHAKPTFR QIMAHFSNVA EAYIEKRNYE
3060 3070 3080 3090 3100
KAYMPRYGIQ RNLTDYSLAR YAFDFYEMTS TTPVRAREAH IQMKAAALRN
3110 3120 3130 3140
VQNRLFGLDG NVGTQEEDTE RHTAGDVNRN MHNLLGVRGV
Note: Produced by conventional translation.
Length:3,140
Mass (Da):355,997
Last modified:November 1, 1996 - v1
Checksum:i837A5A692B56436A
GO
Isoform P3N-PIPO polyprotein (identifier: P0CK04-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0CK04.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting in P3 ORF.
Length:1,026
Mass (Da):116,782
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92280 Genomic RNA. Translation: AAB05823.1.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92280 Genomic RNA. Translation: AAB05823.1.

3D structure databases

ProteinModelPortaliQ84934.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011492. DEAD_Flavivir.
IPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002540. Pept_S30_P1_potyvir.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF07652. Flavi_DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF01577. Peptidase_S30. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLG_PPVSK
AccessioniPrimary (citable) accession number: Q84934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.