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Q848S6 (BLKPC_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbapenem-hydrolyzing beta-lactamase KPC

EC=3.5.2.6
Alternative name(s):
Carbapenem-hydrolyzing beta-lactamase KPC-2
Gene names
Name:bla
Synonyms:kpc, kpc2
Encoded onPlasmid conjugative 70kb
OrganismKlebsiella oxytoca
Taxonomic identifier571 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes carbapenems, penicillins, cephalosporins and aztreonam with varying efficiency.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulation

Not inhibited by EDTA, inhibited by clavulanic acid and tazobactam. Ref.1

Miscellaneous

Initially two different KPC beta-lactamases were identified from two different bacteria (KPC-1 and KPC-2); they were later shown to be identical.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 293269Carbapenem-hydrolyzing beta-lactamase KPC
PRO_0000349144

Regions

Region233 – 2353Substrate binding By similarity

Sites

Active site691Acyl-ester intermediate By similarity
Active site1671Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q848S6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 13EB2FC28005EE5F

FASTA29331,115
        10         20         30         40         50         60 
MSLYRRLVLL SCLSWPLAGF SATALTNLVA EPFAKLEQDF GGSIGVYAMD TGSGATVSYR 

        70         80         90        100        110        120 
AEERFPLCSS FKGFLAAAVL ARSQQQAGLL DTPIRYGKNA LVPWSPISEK YLTTGMTVAE 

       130        140        150        160        170        180 
LSAAAVQYSD NAAANLLLKE LGGPAGLTAF MRSIGDTTFR LDRWELELNS AIPGDARDTS 

       190        200        210        220        230        240 
SPRAVTESLQ KLTLGSALAA PQRQQFVDWL KGNTTGNHRI RAAVPADWAV GDKTGTCGVY 

       250        260        270        280        290 
GTANDYAVVW PTGRAPIVLA VYTRAPNKDD KHSEAVIAAA ARLALEGLGV NGQ 

« Hide

References

[1]"Carbapenem-resistant strain of Klebsiella oxytoca harboring carbapenem-hydrolyzing beta-lactamase KPC-2."
Yigit H., Queenan A.M., Rasheed J.K., Biddle J.W., Domenech-Sanchez A., Alberti S., Bush K., Tenover F.C.
Antimicrob. Agents Chemother. 47:3881-3889(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBSTRATES, ENZYME REGULATION.
Strain: 3127.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY210886 Genomic DNA. Translation: AAO53443.1.

3D structure databases

ProteinModelPortalQ848S6.
SMRQ848S6. Positions 30-293.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ848S6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBLKPC_KLEOX
AccessionPrimary (citable) accession number: Q848S6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families