Q848S6 (BLKPC_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbapenem-hydrolyzing beta-lactamase KPC
EC=3.5.2.6

Alternative name(s):
Carbapenem-hydrolyzing beta-lactamase KPC-2

Gene names

Name:	bla
Synonyms:	kpc, kpc2

Encoded on

Plasmid conjugative 70kb

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes carbapenems, penicillins, cephalosporins and aztreonam with varying efficiency.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Not inhibited by EDTA, inhibited by clavulanic acid and tazobactam. Ref.1
Miscellaneous	Initially two different KPC beta-lactamases were identified from two different bacteria (KPC-1 and KPC-2); they were later shown to be identical.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 293	269	Carbapenem-hydrolyzing beta-lactamase KPC		PRO_0000349144
Regions
	Region	233 – 235	3	Substrate binding By similarity
Sites
	Active site	69	1	Acyl-ester intermediate By similarity
	Active site	167	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q848S6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 13EB2FC28005EE5F	FASTA	293	31,115
	10 20 30 40 50 60 MSLYRRLVLL SCLSWPLAGF SATALTNLVA EPFAKLEQDF GGSIGVYAMD TGSGATVSYR 70 80 90 100 110 120 AEERFPLCSS FKGFLAAAVL ARSQQQAGLL DTPIRYGKNA LVPWSPISEK YLTTGMTVAE 130 140 150 160 170 180 LSAAAVQYSD NAAANLLLKE LGGPAGLTAF MRSIGDTTFR LDRWELELNS AIPGDARDTS 190 200 210 220 230 240 SPRAVTESLQ KLTLGSALAA PQRQQFVDWL KGNTTGNHRI RAAVPADWAV GDKTGTCGVY 250 260 270 280 290 GTANDYAVVW PTGRAPIVLA VYTRAPNKDD KHSEAVIAAA ARLALEGLGV NGQ

References

[1]

"Carbapenem-resistant strain of Klebsiella oxytoca harboring carbapenem-hydrolyzing beta-lactamase KPC-2." Yigit H., Queenan A.M., Rasheed J.K., Biddle J.W., Domenech-Sanchez A., Alberti S., Bush K., Tenover F.C. Antimicrob. Agents Chemother. 47:3881-3889(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBSTRATES, ENZYME REGULATION. Strain: 3127.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY210886 Genomic DNA. Translation: AAO53443.1.
3D structure databases
HSSP	HSSP built from PDB template 1BUE based on UniProtKB P52663.
ProteinModelPortal	Q848S6.
SMR	Q848S6. Positions 30-293.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. False negative. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q848S6.

Entry information

Entry name

BLKPC_KLEOX

Accession

Primary (citable) accession number: Q848S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	March 6, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program