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Q846V6

- SYI_STAEP

UniProt

Q846V6 - SYI_STAEP

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Protein

Isoleucine--tRNA ligase

Gene
ileS
Organism
Staphylococcus epidermidis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
OrganismiStaphylococcus epidermidis
Taxonomic identifieri1282 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Isoleucine--tRNA ligaseUniRule annotationPRO_0000098470Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ846V6.
SMRiQ846V6. Positions 1-864.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q846V6-1 [UniParc]FASTAAdd to Basket

« Hide

MNYKDTLLMP KTDFPMRGGL PNKEPQIQEM WDNDDQYRKA LEKNKNNPSF    50
ILHDGPPYAN GNLHMGHALN KIIKDFIVRY KTMQGFYAPY VPGWDTHGLP 100
IEQALTKKGV DRKKMSVAEF REKCKEFALK QIDIQKKDFK RLGVRGDFNN 150
PYITLTPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY 200
HDKRSASIYV AFDVKDSKGK VDSDAQFIIW TTTPWTIPSN VAITVHPELK 250
YGQYNVDGHK YIVAQALSEE VAEALGWDKD SIQLEKEFTG KELEFVEAQH 300
PFLDRVSLVI NGEHVTTDAG TGCVHTAPGH GEDDYIVGQK YDLPVISPLN 350
DKGVFTEEGG PFEGMFYDKA NKAVTDLLKE KDALLKLDFI THSYPHDWRT 400
KKPVIFRATP QWFASINKVK QDILDAIEDT NFKVDWGKTR IYNMIRDRGE 450
WVISRQRVWG VPLPVFYAEN GDIIMTKETV NHVADLFEKY GSNIWFEKEA 500
KELLPEGFSH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE TRPELSFPAD 550
LYFEGSDQYR GWFNSSITTA VATRGQAPYK FLLSHGFVMD GEGKKMSKSL 600
GNVIVPDQVV KQKGADIARL WVSSTDYLSD VRISDEILKK TSDVYRKIRN 650
TLRFMLGNIN DFNPETDSIA ETNLLEVDRY LLNRLREFTA STINNYENFD 700
YLNIYQEVQN FINVELSNFY LDYGKDILYI EKKDSHKRRS MQTVLYQILI 750
DMTKLLAPIL VHTAEEVWSH TPHVKEESVH LSDMPKVVDV DEELLEKWNT 800
FMNLRDDVNR ALEQARNEKV IGKSLEAKVV IGNNETFNTA EFLQQFNDLQ 850
QLFIVSQVEV KDKVKLKILI R 871
Length:871
Mass (Da):100,134
Last modified:June 1, 2003 - v1
Checksum:i2E140C3F74318A15
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF516209 Genomic DNA. Translation: AAO65848.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF516209 Genomic DNA. Translation: AAO65848.1 .

3D structure databases

ProteinModelPortali Q846V6.
SMRi Q846V6. Positions 1-864.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital."
    Yun H.J., Lee S.W., Yoon G.M., Kim S.Y., Choi S., Lee Y.S., Choi E.C., Kim S.
    J. Antimicrob. Chemother. 51:619-623(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSYI_STAEP
AccessioniPrimary (citable) accession number: Q846V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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