Reviewed, UniProtKB/Swiss-Prot Q845W3 (LEU3_BURM1)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	Bmul_4627, BMULJ_03884

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	3-isopropylmalate dehydrogenase		PRO_0000083673
Regions
	Nucleotide binding	280 – 292	13	NAD By similarity
Sites
	Metal binding	222	1	Magnesium or manganese By similarity
	Metal binding	246	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	222	1	Substrate By similarity
	Site	135	1	Important for catalysis By similarity
	Site	190	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q845W3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6ABAFC839D803186	FASTA	355	38,332
	10 20 30 40 50 60 MKIAVLPGDG IGPEIVTEAV KVLNALDEKF ELEQAPVGGA GYEARGHPLP DATLKLAKEA 70 80 90 100 110 120 DAILFGAVGD WKYDSLERAL RPEQAILGLR KHLELFANFR PAICYPQLVD ASPLKPELVA 130 140 150 160 170 180 GLDILIVREL NGDIYFGQPR GVRAAPDGPF AGEREGFDTM RYSEPEVRRI AHVAFQAAQK 190 200 210 220 230 240 RAKKLLSVDK SNVLETSQFW RDIMIDVSKE YADVELSHMY VDNAAMQLAK APKQFDVIVT 250 260 270 280 290 300 GNMFGDILSD EASMLTGSIG MLPSASLDKN NKGLYEPSHG SAPDIAGKGI ANPLATILSA 310 320 330 340 350 AMLLRYSLNR AEQADRIERA VKTVLEQGYR TGDIATPGCK QVGTAAMGDA VVAAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distribution and organization of auxotrophic genes on the multichromosomal genome of Burkholderia multivorans ATCC 17616." Komatsu H., Imura Y., Ohori A., Nagata Y., Tsuda M. J. Bacteriol. 185:3333-3343(2003) [PubMed: 12754231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 2 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB091435 Genomic DNA. Translation: BAC65259.1. CP000869 Genomic DNA. Translation: ABX18305.1. AP009386 Genomic DNA. Translation: BAG45744.1.
RefSeq	YP_001584597.1. YP_001948280.1.
3D structure databases
HSSP	HSSP built from PDB template 2AYQ based on UniProtKB P12010.
ModBase	Search...
Genome annotation databases
GeneID	5769022. 6362226.
GenomeReviews	Gene locus Bmul_4627 in contig CP000869_GR. Gene locus BMULJ_03884 in contig AP009386_GR.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BURM1

Accession

Primary (citable) accession number: Q845W3
Secondary accession number(s): A9AMB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	June 1, 2003
Last modified:	November 4, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)