Reviewed,
UniProtKB/Swiss-Prot Q845W3 (LEU3_BURM1)
Last modified
February 9, 2010.
Version 48.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-isopropylmalate dehydrogenase EC=1.1.1.85 Alternative name(s): Beta-IPM dehydrogenase Short name=IMDH 3-IPM-DH | ||||
| Gene names |
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| Organism | Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 395019 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex |
Protein attributes
| Sequence length | 355 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033 |
| Catalytic activity | (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033 |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_01033 |
| Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033 |
| Subunit structure | Homodimer By similarity. HAMAP MF_01033 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_01033. |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH bindingInferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 355 | 355 | 3-isopropylmalate dehydrogenase HAMAP MF_01033 | PRO_0000083673 | |||||
Regions | |||||||||
| Nucleotide binding | 280 – 292 | 13 | NAD By similarity | ||||||
Sites | |||||||||
| Metal binding | 222 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 246 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 250 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 90 | 1 | Substrate By similarity | ||||||
| Binding site | 100 | 1 | Substrate By similarity | ||||||
| Binding site | 128 | 1 | Substrate By similarity | ||||||
| Binding site | 222 | 1 | Substrate By similarity | ||||||
| Site | 135 | 1 | Important for catalysis By similarity | ||||||
| Site | 190 | 1 | Important for catalysis By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Distribution and organization of auxotrophic genes on the multichromosomal genome of Burkholderia multivorans ATCC 17616." Komatsu H., Imura Y., Ohori A., Nagata Y., Tsuda M. J. Bacteriol. 185:3333-3343(2003) [PubMed: 12754231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Complete sequence of chromosome 2 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB091435 Genomic DNA. Translation: BAC65259.1. CP000869 Genomic DNA. Translation: ABX18305.1. AP009386 Genomic DNA. Translation: BAG45744.1. |
| RefSeq | YP_001584597.1. YP_001948280.1. |
3D structure databases | |
| SMR | Q845W3. Positions 2-355. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 5769022. 6362226. |
| GenomeReviews | Gene locus BMULJ_03884 in contig AP009386_GR. Gene locus Bmul_4627 in contig CP000869_GR. |
| KEGG | bmj:BMULJ_03884. bmu:Bmul_4627. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG518924. |
| OMA | NTAFGLY. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.85. 74079. |
Family and domain databases | |
| HAMAP | MF_01033. LeuB_type1. [Tree] |
| InterPro | IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00169. leuB. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LEU3_BURM1 | ||||||||
| Accession | Primary (citable) accession number: Q845W3 Secondary accession number(s): A9AMB2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


