Q845U8 (HIS4_BURM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	Bmul_0333, BMULJ_02921

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia multivorans

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000141991
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	131	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q845U8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 79A2F1C2637D6D51	FASTA	251	26,733
	10 20 30 40 50 60 MLLIPAIDLK DGQCVRLKQG DMDQATIFSE DPAAMARKWV DLGARRLHLV DLNGAFAGKP 70 80 90 100 110 120 KNLDAIEAIL DEVGDEIPVQ LGGGIRSLET IEKYLDAGLS YVIIGTAAVK DPGFLRDACT 130 140 150 160 170 180 AFQGNIIVGL DAKDGKVATD GWSKLTGHEV IDLAQKFEDY GVESIVYTDI GRDGMLQGIN 190 200 210 220 230 240 IDATVKLAQA VGIPVIASGG LSNLEDIDKL CEVEEEGVEG VICGRAIYSG DLDFAAAQRR 250 ADELNGELDD A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distribution and organization of auxotrophic genes on the multichromosomal genome of Burkholderia multivorans ATCC 17616." Komatsu H., Imura Y., Ohori A., Nagata Y., Tsuda M. J. Bacteriol. 185:3333-3343(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.
[3]	"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB091436 Genomic DNA. Translation: BAC65275.1. CP000868 Genomic DNA. Translation: ABX14028.1. AP009385 Genomic DNA. Translation: BAG44806.1.
RefSeq	YP_001578525.1. NC_010084.1. YP_001947342.1. NC_010804.1.
3D structure databases
ProteinModelPortal	Q845U8.
ModBase	Search...
Protein-protein interaction databases
STRING	395019.Bmul_0333.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX14028; ABX14028; Bmul_0333. BAG44806; BAG44806; BMULJ_02921.
GeneID	5765142. 6359508.
KEGG	bmj:BMULJ_02921. bmu:Bmul_0333.
PATRIC	19167804. VBIBurMul203716_3951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
OMA	DGHCVRL.
ProtClustDB	PRK00748.
Enzyme and pathway databases
BioCyc	BMUL395019:GIYO-2919-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR006063. HisA. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00007. TIGR00007. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_BURM1

Accession

Primary (citable) accession number: Q845U8
Secondary accession number(s): A9AE06

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program