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Protein

mRNA-capping enzyme

Gene

A103R

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

mRNA capping. Transfers a GMP cap onto the end of mRNA that terminates with a 5'-diphosphate tail.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821N6-GMP-lysine intermediate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.50. 4540.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-capping enzyme
Alternative name(s):
GTP--RNA guanylyltransferase
mRNA guanylyltransferase (EC:2.7.7.50)
Gene namesi
Ordered Locus Names:A103R
OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
Taxonomic identifieri10506 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 114049]
Proteomesi
  • UP000000862 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330mRNA-capping enzymePRO_0000210107Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
330
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 218Combined sources
Beta strandi24 – 318Combined sources
Helixi33 – 4614Combined sources
Beta strandi57 – 637Combined sources
Helixi66 – 683Combined sources
Helixi69 – 746Combined sources
Beta strandi77 – 837Combined sources
Beta strandi85 – 9511Combined sources
Beta strandi98 – 1047Combined sources
Beta strandi110 – 1123Combined sources
Helixi122 – 1243Combined sources
Beta strandi126 – 13510Combined sources
Turni136 – 1394Combined sources
Beta strandi140 – 15112Combined sources
Helixi161 – 17212Combined sources
Beta strandi181 – 1877Combined sources
Helixi196 – 20914Combined sources
Beta strandi212 – 22211Combined sources
Beta strandi227 – 2359Combined sources
Beta strandi243 – 2475Combined sources
Turni250 – 2523Combined sources
Beta strandi254 – 2574Combined sources
Turni259 – 2613Combined sources
Beta strandi264 – 2663Combined sources
Beta strandi279 – 2857Combined sources
Beta strandi288 – 2947Combined sources
Helixi304 – 31613Combined sources
Helixi320 – 3234Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CKMX-ray2.50A/B1-330[»]
1CKNX-ray2.50A/B1-330[»]
1CKOX-ray3.10A1-330[»]
ProteinModelPortaliQ84424.
SMRiQ84424. Positions 11-327.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ84424.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic GTase family.Curated

Phylogenomic databases

KOiK13917.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR001339. mRNA_cap_enzyme.
IPR013846. mRNA_cap_enzyme_C.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF03919. mRNA_cap_C. 1 hit.
PF01331. mRNA_cap_enzyme. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

Q84424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPPTINTGK NITTERAVLT LNGLQIKLHK VVGESRDDIV AKMKDLAMDD
60 70 80 90 100
HKFPRLPGPN PVSIERKDFE KLKQNKYVVS EKTDGIRFMM FFTRVFGFKV
110 120 130 140 150
CTIIDRAMTV YLLPFKNIPR VLFQGSIFDG ELCVDIVEKK FAFVLFDAVV
160 170 180 190 200
VSGVTVSQMD LASRFFAMKR SLKEFKNVPE DPAILRYKEW IPLEHPTIIK
210 220 230 240 250
DHLKKANAIY HTDGLIIMSV DEPVIYGRNF NLFKLKPGTH HTIDFIIMSE
260 270 280 290 300
DGTIGIFDPN LRKNVPVGKL DGYYNKGSIV ECGFADGTWK YIQGRSDKNQ
310 320 330
ANDRLTYEKT LLNIEENITI DELLDLFKWE
Length:330
Mass (Da):37,832
Last modified:November 1, 1996 - v1
Checksum:i6AF8A404710812D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF411744 Genomic DNA. Translation: AAC96471.1.
PIRiT17593.
RefSeqiNP_048451.1. NC_000852.5.

Genome annotation databases

GeneIDi918242.
KEGGivg:918242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF411744 Genomic DNA. Translation: AAC96471.1.
PIRiT17593.
RefSeqiNP_048451.1. NC_000852.5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CKMX-ray2.50A/B1-330[»]
1CKNX-ray2.50A/B1-330[»]
1CKOX-ray3.10A1-330[»]
ProteinModelPortaliQ84424.
SMRiQ84424. Positions 11-327.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi918242.
KEGGivg:918242.

Phylogenomic databases

KOiK13917.

Enzyme and pathway databases

BRENDAi2.7.7.50. 4540.

Miscellaneous databases

EvolutionaryTraceiQ84424.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR001339. mRNA_cap_enzyme.
IPR013846. mRNA_cap_enzyme_C.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF03919. mRNA_cap_C. 1 hit.
PF01331. mRNA_cap_enzyme. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of 43 kb of the Chlorella virus PBCV-1 330-kb genome: map positions 45 to 88."
    Li Y., Lu Z., Burbank D.E., Kutish G.F., Rock D.L., Etten J.L.
    Virology 212:134-150(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes."
    Haakansson K., Doherty A.J., Shuman S., Wigley D.B.
    Cell 89:545-553(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  3. "Structure of a complex between a cap analogue and mRNA guanylyl transferase demonstrates the structural chemistry of RNA capping."
    Haakansson K., Wigley D.B.
    Proc. Natl. Acad. Sci. U.S.A. 95:1505-1510(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 11-327.

Entry informationi

Entry nameiMCE_PBCV1
AccessioniPrimary (citable) accession number: Q84424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.