Q84173 (DPOL_ORFN2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase
EC=2.7.7.7

Gene names

Name:

POL

Organism

Orf virus (strain NZ2) (OV NZ-2)

Taxonomic identifier

10259 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Parapoxvirus › Orf virus

Virus host

Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Ovis aries (Sheep) [TaxID: 9940]

Protein attributes

Sequence length	1008 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes DNA synthesis. Acquires processivity by associating with a heterodimeric processivity factor comprised of the viral A20 and D4 proteins, thereby forming the DNA polymerase holoenzyme. Displays 3'- to 5' exonuclease activity. Might participate in viral DNA recombination. Does not perform translesion synthesis across an abasic site By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Interacts with A20. Component of the Uracil-DNA glycosylase(UDG)-A20-polymerase complex; A20 and UDG form a heterodimeric processivity factor that associates with E9 to form the processive polymerase holoenzyme By similarity.
Sequence similarities	Belongs to the DNA polymerase type-B family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1008	1008	DNA polymerase		PRO_0000046536

Sequences

	Sequence		Length	Mass (Da)
	Q84173 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3C9A0450DA1990D1	FASTA	1,008	113,955
	10 20 30 40 50 60 MELKCLNWFE NRGNDSRFLF LKARRADNAV VYLRFVQHFY YVVRADAVAD IAQPLAWTRA 70 80 90 100 110 120 LGPMSVVSID EIVARSAKIP ERQRSEIELC LVASERKLAP PEVFMSDFLN VSWFFVAHDI 130 140 150 160 170 180 DPDGCYRVDP ALLRDLGSNC FHCDDPGACF AEKIPRFNVT RSGLFLDIEC HFEKKFPSVF 190 200 210 220 230 240 KNAVSHISFC VVDKDGAERR FTLTNSDMLS DADLEEAARR EIPVCLDPAD VKFDAEVTLC 250 260 270 280 290 300 PEVTLLRVAK RLLEMPLDFV VTFNGHNFDL RYLDSRLSLL TGEHIRFRLP DGTETVNFCV 310 320 330 340 350 360 YERTKSSHKG VGGVSSTTFH INNNNGTIYF DLYAFIQRTE KLDSYKLDAI SKNAFHCTAV 370 380 390 400 410 420 VEDARPDAVR FRGDRSTDAD GNAAVFARVL STGNYVTVDE RVCRVLHKRV DEDGFTVDLA 430 440 450 460 470 480 DPAARAPGDR VTLAFGKDDV SLADMYANYS LDVCLDMARY CLHDACLCLY LWSHYGVETK 490 500 510 520 530 540 IAAAASTYLL PQSVVFEYRA STCIKGPLMK LLLENRTVMV RADTKSKFFY EGGRVMAPKQ 550 560 570 580 590 600 KMHNKHVLIF DYNSLYPNVC IYANLSPETL VGVVVSDNRL DAEVAAVDVR RMFPAPRYIA 610 620 630 640 650 660 VPCEPRSPEL VAEVAIFDRE AKGIIPMLLR SFLDARAKYK KLMKAAETAV DREIYNSMQY 670 680 690 700 710 720 TYKITANSVY GLMGFRNSAL FSYASAKSCT AIGRTMIAYL ERTLDGASVC GTRLSLAAAP 730 740 750 760 770 780 DNPLLRDEAF AGRAAELEID AAVAGERTER VGFRSVYGDT DSVFLEVGAS DIAFSRRVGR 790 800 810 820 830 840 CLERVINEHV LFANFKVEFE AVYCNLIMQS KKKYTTIKFA VSDGGGSERV SKGTSETRRD 850 860 870 880 890 900 VAPFHKLMIR KYKDMLCRAL AEEGSGNVGV EILRSLEDEL TFEFEARSMP LDWFLLSRTH 910 920 930 940 950 960 HKNFKSPDNP NVALVTRYNA ANAEAIEIGE RYFFAYVCEE GPWRRRIANV KSYERVVDKT 970 980 990 1000 FRLDKNERIM YEVYFKRLCT EIVNLLDNKA MCTLFFERLF GSKPVFTG

References

[1]	"Location, DNA sequence and transcriptional analysis of the DNA polymerase gene of orf virus." Mercer A.A., Green G., Sullivan J.T., Robinson A.J., Drillien R. J. Gen. Virol. 77:1563-1568(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49979 Genomic DNA. Translation: AAB19092.1.
3D structure databases
ProteinModelPortal	Q84173.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.1600.10. 2 hits.
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR017964. DNA-dir_DNA_pol_B_CS. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006134. DNA-dir_DNA_pol_B_multi_dom. IPR013617. DNA-dir_DNA_pol_B_vir_insert. IPR023211. DNA_pol_palm_dom. IPR013660. DNApol_B_exo_N. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF00136. DNA_pol_B. 1 hit. PF08408. DNA_pol_B_3. 1 hit. PF03104. DNA_pol_B_exo1. 1 hit. PF08452. DNAP_B_exo_N. 1 hit. [Graphical view]
PRINTS	PR00106. DNAPOLB.
SMART	SM00486. POLBc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS00116. DNA_POLYMERASE_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOL_ORFN2

Accession

Primary (citable) accession number: Q84173

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program