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Protein

Replicase large subunit

Gene
N/A
Organism
Odontoglossum ringspot virus (isolate Singapore 1) (ORSV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).By similarityCurated

Miscellaneous

This protein is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for Leu-1112 and Gln-1114, this results in the addition of the RdRp region to the replicase.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi826 – 833ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
183 kDa protein
RNA-directed RNA polymerase
Cleaved into the following chain:
Alternative name(s):
126 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
OrganismiOdontoglossum ringspot virus (isolate Singapore 1) (ORSV)
Taxonomic identifieri138662 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageVirgaviridaeTobamovirus
Virus hostiCymbidium [TaxID: 14366]
Odontoglossum [TaxID: 154697]
Proteomesi
  • UP000008384 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000411781 – 1612Replicase large subunitAdd BLAST1612
ChainiPRO_00000411791 – 1112Replicase small subunitAdd BLAST1112

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ84133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 280Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST209
Domaini794 – 953(+)RNA virus helicase ATP-bindingAdd BLAST160
Domaini954 – 1112(+)RNA virus helicase C-terminalAdd BLAST159
Domaini1374 – 1487RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 452MethyltransferaseAdd BLAST403
Regioni822 – 1080HelicaseAdd BLAST259

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000048.

Family and domain databases

InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHFQQTMNN KVIEAGMGRN SLINDLAQRR VYDNAVEELN HRSRRPRVNF
60 70 80 90 100
SKVISQEQII QATNAYPEFE ITFYNTQLAV HSMAGGLRAL ELEYLMMQIP
110 120 130 140 150
FGSITYDIGG NFSAHLYKGR DYVHCCMPNL DIRDVARHIN QQDTVSTYLA
160 170 180 190 200
RLERSKRGLP VFQQSAFNKY MNDPDAVCCD KRFQDCSYSA GLPGKTYAVG
210 220 230 240 250
LHSIYDIPAD EFGAALLRKD VHICYAAFHF SENLLLETTS APLDEIGATF
260 270 280 290 300
YKSGDRLSFF FQNESTLNYE HSYKNVIKYV CKTFFPASNR FVYHKEFMCT
310 320 330 340 350
RVNTWFCKFT KVDTYFLFRG VYTRGEDSEQ FYTAMDEAWE YKKTLAMLNS
360 370 380 390 400
ERTIFRDRAA VNFWFPKVKD MVIVPLFDGS VTSGKMKRSE VMVNKDFVYT
410 420 430 440 450
VLNHIRTYQD KALTYKNVLS FVESIRSRVI INGVTARSEW DVDKSVLQAL
460 470 480 490 500
SMTFLLQTKL AEAKDQVVLK KFQKFDDTVT NLFWKQISDA VGDLFPSIKE
510 520 530 540 550
RLISGGFVKV AEQSLQIKTP DEYITPADKL VMEYQATEEL QHLDISKPLE
560 570 580 590 600
RAEKYYNALS ELSVLKECDE FDITQFKNLC EEKDIAPDVV AKVIVPIMKN
610 620 630 640 650
ELTLPFKNPT PEALSDALSP LPKDLDMRFC LLKLSTCAPF PSVKTLDSGL
660 670 680 690 700
LPKQSYGDER QFESQSVVSV SDFHLKSVES VKMKSMSSAV YTGPLKVQQM
710 720 730 740 750
KNYMDYLSAS ISATVSNLCK VLKDVYGADP ESAEKSGVYD VVKGKWLLKP
760 770 780 790 800
KDKCHAWGVA ELNNGEKVIV LLEWADGFPI CGDWRRVAVS SDSPIYSDMG
810 820 830 840 850
KLQTLLSCLK DGEPVLRMPK VTLVDGVPGC GKTKEILETV NFDEDLILVP
860 870 880 890 900
GKEACKMIIK RANKSGHVRA TRDNVRTVDS FLMHLKPKTY NKLFIDEGLM
910 920 930 940 950
LHTGCVNFLV ALSHCREAMV FGDAEQIPFI NRVANFPYPK HFRYTCLYHR
960 970 980 990 1000
EVRRLSLRCP ADVTHFMNSK YDGKVLCTND VIRSVDAEVV RGKGVFNPKS
1010 1020 1030 1040 1050
KPLKGKIITF TQSDKAELKE RGYEEVSTFG EINTVHEIQG ETFEDVSVVR
1060 1070 1080 1090 1100
LTPTPLELIS KSSPHVLVAL TRHTKSFKYY SVVLDPLVKV WSDLSKVSDF
1110 1120 1130 1140 1150
ILDMYKVDAG ILXQLQVGSI FKGENLFVPC PKSGYISDMQ TYYDTLVPGN
1160 1170 1180 1190 1200
STILNEYDAV TMNLRENNLN VKDCTIDFSK SVSVPRQQQE FFTPAHRTAA
1210 1220 1230 1240 1250
ERPRSAGLLE NLVAMIKRNF NSPDLTGILD IEDTAELVVN KFWDAYIIDE
1260 1270 1280 1290 1300
LSGGNVTPMT SDAFHRWMAK QEKSTIGQLA DFDFVDLPAI DQYKHMIKAQ
1310 1320 1330 1340 1350
PKQKLGLSPQ DEYAALQTIV YHSKQINAIF GPLFSELTRQ LLERIDSSKF
1360 1370 1380 1390 1400
LFYTRKTPEQ IEAFFSDLDS TVPMEVLELD ISKYDKSQNE FHCAVEYLIW
1410 1420 1430 1440 1450
EKLGLNGFLE EVWKQGHRKT SLKDYTAGIK TCLWYQRKSG DVTTFIGNTV
1460 1470 1480 1490 1500
IIAACLASMI PMDKVIKAAF CGDDSILYIP KGLDLPDIQS GANLMWNFEA
1510 1520 1530 1540 1550
KLYRKRYGYF CGRYIIHHDR GAIVYYDPLK LISKLGCKHI KSLDHLEEFR
1560 1570 1580 1590 1600
ISLCDVSSSL NNCAYFGQLN DAIAEVHKTA VNGSFAFCSI VKYLSDKNLF
1610
RTLFNNGSST KG
Length:1,612
Mass (Da):183,196
Last modified:December 1, 2000 - v2
Checksum:i3B444670B0ACB189
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34586 Genomic RNA. Translation: AAC55012.2.

Keywords - Coding sequence diversityi

RNA suppression of termination

Similar proteinsi

Entry informationi

Entry nameiRDRP_ORSVS
AccessioniPrimary (citable) accession number: Q84133
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 12, 2017
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families