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Q83WS0

- FTHS_METEA

UniProt

Q83WS0 - FTHS_METEA

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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.1 Publication

Kineticsi

  1. KM=22 mM for formate1 Publication
  2. KM=0.8 mM for tetrahydrofolate1 Publication
  3. KM=21 µM for ATP1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3942.
MEXT272630:GBY6-313-MONOMER.
BRENDAi6.3.4.3. 3296.
SABIO-RKQ83WS0.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase (EC:6.3.4.3)
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name:
FHS
Short name:
FTHFS
Gene namesi
Name:fhs
Synonyms:ftfL
Ordered Locus Names:MexAM1_META1p0329
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 557556Formate--tetrahydrofolate ligasePRO_0000199359Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ83WS0.
SMRiQ83WS0. Positions 4-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiLKHHGGV.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q83WS0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG
60 70 80 90 100
KPEGKLVLVT AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP
110 120 130 140 150
CFGMKGGAAG GGKAQVVPME QINLHFTGDF HAITSAHSLA AALIDNHIYW
160 170 180 190 200
ANELNIDVRR IHWRRVVDMN DRALRAINQS LGGVANGFPR EDGFDITVAS
210 220 230 240 250
EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA TGAMTVLLKD
260 270 280 290 300
ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG
310 320 330 340 350
FGADLGAEKF IDIKCRQTGL KPSAVVIVAT IRALKMHGGV NKKDLQAENL
360 370 380 390 400
DALEKGFANL ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL
410 420 430 440 450
QVEAITCKHW AEGGAGAEAL AQAVVKLAEG EQKPLTFAYE TETKITDKIK
460 470 480 490 500
AIATKLYGAA DIQIESKAAT KLAGFEKDGY GGLPVCMAKT QYSFSTDPTL
510 520 530 540 550
MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP AADTIRLDAN

GQIDGLF
Length:557
Mass (Da):59,391
Last modified:January 23, 2007 - v3
Checksum:iD0DB51A90971072A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY279316 Genomic DNA. Translation: AAP33693.1.
CP001510 Genomic DNA. Translation: ACS38281.1.
RefSeqiWP_003606333.1. NC_012808.1.
YP_002961558.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS38281; ACS38281; MexAM1_META1p0329.
GeneIDi7990350.
KEGGimea:Mex_1p0329.
PATRICi22506475. VBIMetExt101010_0354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY279316 Genomic DNA. Translation: AAP33693.1 .
CP001510 Genomic DNA. Translation: ACS38281.1 .
RefSeqi WP_003606333.1. NC_012808.1.
YP_002961558.1. NC_012808.1.

3D structure databases

ProteinModelPortali Q83WS0.
SMRi Q83WS0. Positions 4-555.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS38281 ; ACS38281 ; MexAM1_META1p0329 .
GeneIDi 7990350.
KEGGi mea:Mex_1p0329.
PATRICi 22506475. VBIMetExt101010_0354.

Phylogenomic databases

eggNOGi COG2759.
HOGENOMi HOG000040280.
KOi K01938.
OMAi LKHHGGV.
OrthoDBi EOG6PCPSP.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci MetaCyc:MONOMER-3942.
MEXT272630:GBY6-313-MONOMER.
BRENDAi 6.3.4.3. 3296.
SABIO-RK Q83WS0.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth."
    Marx C.J., Laukel M., Vorholt J.A., Lidstrom M.E.
    J. Bacteriol. 185:7169-7175(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.

Entry informationi

Entry nameiFTHS_METEA
AccessioniPrimary (citable) accession number: Q83WS0
Secondary accession number(s): C5AS46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 58 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3