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Q83WS0

- FTHS_METEA

UniProt

Q83WS0 - FTHS_METEA

Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.1 Publication

    Kineticsi

    1. KM=22 mM for formate1 Publication
    2. KM=0.8 mM for tetrahydrofolate1 Publication
    3. KM=21 µM for ATP1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi65 – 728ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3942.
    MEXT272630:GBY6-313-MONOMER.
    BRENDAi6.3.4.3. 3296.
    SABIO-RKQ83WS0.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate--tetrahydrofolate ligase (EC:6.3.4.3)
    Alternative name(s):
    Formyltetrahydrofolate synthetase
    Short name:
    FHS
    Short name:
    FTHFS
    Gene namesi
    Name:fhs
    Synonyms:ftfL
    Ordered Locus Names:MexAM1_META1p0329
    OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
    Taxonomic identifieri272630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000009081: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 557556Formate--tetrahydrofolate ligasePRO_0000199359Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ83WS0.
    SMRiQ83WS0. Positions 4-555.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2759.
    HOGENOMiHOG000040280.
    KOiK01938.
    OMAiLKHHGGV.
    OrthoDBiEOG6PCPSP.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q83WS0-1 [UniParc]FASTAAdd to Basket

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    MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG    50
    KPEGKLVLVT AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP 100
    CFGMKGGAAG GGKAQVVPME QINLHFTGDF HAITSAHSLA AALIDNHIYW 150
    ANELNIDVRR IHWRRVVDMN DRALRAINQS LGGVANGFPR EDGFDITVAS 200
    EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA TGAMTVLLKD 250
    ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG 300
    FGADLGAEKF IDIKCRQTGL KPSAVVIVAT IRALKMHGGV NKKDLQAENL 350
    DALEKGFANL ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL 400
    QVEAITCKHW AEGGAGAEAL AQAVVKLAEG EQKPLTFAYE TETKITDKIK 450
    AIATKLYGAA DIQIESKAAT KLAGFEKDGY GGLPVCMAKT QYSFSTDPTL 500
    MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP AADTIRLDAN 550
    GQIDGLF 557
    Length:557
    Mass (Da):59,391
    Last modified:January 23, 2007 - v3
    Checksum:iD0DB51A90971072A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY279316 Genomic DNA. Translation: AAP33693.1.
    CP001510 Genomic DNA. Translation: ACS38281.1.
    RefSeqiYP_002961558.1. NC_012808.1.

    Genome annotation databases

    EnsemblBacteriaiACS38281; ACS38281; MexAM1_META1p0329.
    GeneIDi7990350.
    KEGGimea:Mex_1p0329.
    PATRICi22506475. VBIMetExt101010_0354.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY279316 Genomic DNA. Translation: AAP33693.1 .
    CP001510 Genomic DNA. Translation: ACS38281.1 .
    RefSeqi YP_002961558.1. NC_012808.1.

    3D structure databases

    ProteinModelPortali Q83WS0.
    SMRi Q83WS0. Positions 4-555.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS38281 ; ACS38281 ; MexAM1_META1p0329 .
    GeneIDi 7990350.
    KEGGi mea:Mex_1p0329.
    PATRICi 22506475. VBIMetExt101010_0354.

    Phylogenomic databases

    eggNOGi COG2759.
    HOGENOMi HOG000040280.
    KOi K01938.
    OMAi LKHHGGV.
    OrthoDBi EOG6PCPSP.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci MetaCyc:MONOMER-3942.
    MEXT272630:GBY6-313-MONOMER.
    BRENDAi 6.3.4.3. 3296.
    SABIO-RK Q83WS0.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_01543. FTHFS.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth."
      Marx C.J., Laukel M., Vorholt J.A., Lidstrom M.E.
      J. Bacteriol. 185:7169-7175(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14718 / DSM 1338 / AM1.

    Entry informationi

    Entry nameiFTHS_METEA
    AccessioniPrimary (citable) accession number: Q83WS0
    Secondary accession number(s): C5AS46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3