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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.1 Publication

Kineticsi

  1. KM=22 mM for formate1 Publication
  2. KM=0.8 mM for tetrahydrofolate1 Publication
  3. KM=21 µM for ATP1 Publication

    Pathway: tetrahydrofolate interconversion

    This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
    View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi65 – 728ATPBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3942.
    MEXT272630:GBY6-313-MONOMER.
    BRENDAi6.3.4.3. 3296.
    SABIO-RKQ83WS0.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate--tetrahydrofolate ligase (EC:6.3.4.3)
    Alternative name(s):
    Formyltetrahydrofolate synthetase
    Short name:
    FHS
    Short name:
    FTHFS
    Gene namesi
    Name:fhs
    Synonyms:ftfL
    Ordered Locus Names:MexAM1_META1p0329
    OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
    Taxonomic identifieri272630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000009081 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 557556Formate--tetrahydrofolate ligasePRO_0000199359Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi272630.MexAM1_META1p0329.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83WS0.
    SMRiQ83WS0. Positions 4-555.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2759.
    HOGENOMiHOG000040280.
    KOiK01938.
    OMAiCMVKTHL.
    OrthoDBiEOG6PCPSP.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q83WS0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG
    60 70 80 90 100
    KPEGKLVLVT AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP
    110 120 130 140 150
    CFGMKGGAAG GGKAQVVPME QINLHFTGDF HAITSAHSLA AALIDNHIYW
    160 170 180 190 200
    ANELNIDVRR IHWRRVVDMN DRALRAINQS LGGVANGFPR EDGFDITVAS
    210 220 230 240 250
    EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA TGAMTVLLKD
    260 270 280 290 300
    ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG
    310 320 330 340 350
    FGADLGAEKF IDIKCRQTGL KPSAVVIVAT IRALKMHGGV NKKDLQAENL
    360 370 380 390 400
    DALEKGFANL ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL
    410 420 430 440 450
    QVEAITCKHW AEGGAGAEAL AQAVVKLAEG EQKPLTFAYE TETKITDKIK
    460 470 480 490 500
    AIATKLYGAA DIQIESKAAT KLAGFEKDGY GGLPVCMAKT QYSFSTDPTL
    510 520 530 540 550
    MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP AADTIRLDAN

    GQIDGLF
    Length:557
    Mass (Da):59,391
    Last modified:January 23, 2007 - v3
    Checksum:iD0DB51A90971072A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY279316 Genomic DNA. Translation: AAP33693.1.
    CP001510 Genomic DNA. Translation: ACS38281.1.
    RefSeqiWP_003606333.1. NC_012808.1.
    YP_002961558.1. NC_012808.1.

    Genome annotation databases

    EnsemblBacteriaiACS38281; ACS38281; MexAM1_META1p0329.
    KEGGimea:Mex_1p0329.
    PATRICi22506475. VBIMetExt101010_0354.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY279316 Genomic DNA. Translation: AAP33693.1.
    CP001510 Genomic DNA. Translation: ACS38281.1.
    RefSeqiWP_003606333.1. NC_012808.1.
    YP_002961558.1. NC_012808.1.

    3D structure databases

    ProteinModelPortaliQ83WS0.
    SMRiQ83WS0. Positions 4-555.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi272630.MexAM1_META1p0329.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiACS38281; ACS38281; MexAM1_META1p0329.
    KEGGimea:Mex_1p0329.
    PATRICi22506475. VBIMetExt101010_0354.

    Phylogenomic databases

    eggNOGiCOG2759.
    HOGENOMiHOG000040280.
    KOiK01938.
    OMAiCMVKTHL.
    OrthoDBiEOG6PCPSP.

    Enzyme and pathway databases

    UniPathwayiUPA00193.
    BioCyciMetaCyc:MONOMER-3942.
    MEXT272630:GBY6-313-MONOMER.
    BRENDAi6.3.4.3. 3296.
    SABIO-RKQ83WS0.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth."
      Marx C.J., Laukel M., Vorholt J.A., Lidstrom M.E.
      J. Bacteriol. 185:7169-7175(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14718 / DSM 1338 / AM1.

    Entry informationi

    Entry nameiFTHS_METEA
    AccessioniPrimary (citable) accession number: Q83WS0
    Secondary accession number(s): C5AS46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: January 23, 2007
    Last modified: June 24, 2015
    This is version 61 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.