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Q83WS0

- FTHS_METEA

UniProt

Q83WS0 - FTHS_METEA

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Protein

Formate--tetrahydrofolate ligase

Gene
fhs, ftfL, MexAM1_META1p0329
Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.1 Publication

Kineticsi

  1. KM=22 mM for formate1 Publication
  2. KM=0.8 mM for tetrahydrofolate
  3. KM=21 µM for ATP

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3942.
MEXT272630:GBY6-313-MONOMER.
BRENDAi6.3.4.3. 3296.
SABIO-RKQ83WS0.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase (EC:6.3.4.3)
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name:
FHS
Short name:
FTHFS
Gene namesi
Name:fhs
Synonyms:ftfL
Ordered Locus Names:MexAM1_META1p0329
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 557556Formate--tetrahydrofolate ligaseUniRule annotationPRO_0000199359Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ83WS0.
SMRiQ83WS0. Positions 4-555.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiLKHHGGV.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q83WS0-1 [UniParc]FASTAAdd to Basket

« Hide

MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG    50
KPEGKLVLVT AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP 100
CFGMKGGAAG GGKAQVVPME QINLHFTGDF HAITSAHSLA AALIDNHIYW 150
ANELNIDVRR IHWRRVVDMN DRALRAINQS LGGVANGFPR EDGFDITVAS 200
EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA TGAMTVLLKD 250
ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG 300
FGADLGAEKF IDIKCRQTGL KPSAVVIVAT IRALKMHGGV NKKDLQAENL 350
DALEKGFANL ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL 400
QVEAITCKHW AEGGAGAEAL AQAVVKLAEG EQKPLTFAYE TETKITDKIK 450
AIATKLYGAA DIQIESKAAT KLAGFEKDGY GGLPVCMAKT QYSFSTDPTL 500
MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP AADTIRLDAN 550
GQIDGLF 557
Length:557
Mass (Da):59,391
Last modified:January 23, 2007 - v3
Checksum:iD0DB51A90971072A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY279316 Genomic DNA. Translation: AAP33693.1.
CP001510 Genomic DNA. Translation: ACS38281.1.
RefSeqiYP_002961558.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS38281; ACS38281; MexAM1_META1p0329.
GeneIDi7990350.
KEGGimea:Mex_1p0329.
PATRICi22506475. VBIMetExt101010_0354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY279316 Genomic DNA. Translation: AAP33693.1 .
CP001510 Genomic DNA. Translation: ACS38281.1 .
RefSeqi YP_002961558.1. NC_012808.1.

3D structure databases

ProteinModelPortali Q83WS0.
SMRi Q83WS0. Positions 4-555.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS38281 ; ACS38281 ; MexAM1_META1p0329 .
GeneIDi 7990350.
KEGGi mea:Mex_1p0329.
PATRICi 22506475. VBIMetExt101010_0354.

Phylogenomic databases

eggNOGi COG2759.
HOGENOMi HOG000040280.
KOi K01938.
OMAi LKHHGGV.
OrthoDBi EOG6PCPSP.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci MetaCyc:MONOMER-3942.
MEXT272630:GBY6-313-MONOMER.
BRENDAi 6.3.4.3. 3296.
SABIO-RK Q83WS0.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth."
    Marx C.J., Laukel M., Vorholt J.A., Lidstrom M.E.
    J. Bacteriol. 185:7169-7175(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.

Entry informationi

Entry nameiFTHS_METEA
AccessioniPrimary (citable) accession number: Q83WS0
Secondary accession number(s): C5AS46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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