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Protein

Glycine/sarcosine N-methyltransferase

Gene
N/A
Organism
Aphanothece halophytica
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of glycine and sarcosine to sarcosine and dimethylglycine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.1 Publication
2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine.1 Publication

Enzyme regulationi

Inhibited by actate, dimethylglycine and S-adenosyl-L-homocysteine.1 Publication

Kineticsi

  1. KM=0.6 mM for S-adenosyl-L-homocysteine (at pH 8.8 and at 37 degrees Celsius)1 Publication
  2. KM=0.8 mM for sarcosine (at pH 8.8 and at 37 degrees Celsius)1 Publication
  3. KM=1.5 mM for glycine (at pH 8.8 and at 37 degrees Celsius)1 Publication
  1. Vmax=0.50 µmol/min/mg enzyme (at pH 8.8 and at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.8. The activities remain high at more alkaline pH but decrease sharply at the acidic side of the optimal pH.1 Publication

Pathwayi: betaine biosynthesis via glycine pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes betaine from glycine.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycine/sarcosine N-methyltransferase
  2. Glycine/sarcosine N-methyltransferase, Sarcosine/dimethylglycine N-methyltransferase
  3. Sarcosine/dimethylglycine N-methyltransferase
This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei36 – 361S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei45 – 451S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei69 – 691S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei90 – 901S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei134 – 1341S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei136 – 1361SubstratePROSITE-ProRule annotation
Binding sitei169 – 1691SubstrateCurated
Binding sitei208 – 2081SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  • glycine N-methyltransferase activity Source: UniProtKB
  • sarcosine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • glycine betaine biosynthetic process from glycine Source: UniProtKB
  • methylation Source: UniProtKB

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8562.
BRENDAi2.1.1.156. 383.
UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine/sarcosine N-methyltransferase (EC:2.1.1.156)
OrganismiAphanothece halophytica
Taxonomic identifieri72020 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesAphanothece

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi169 – 1691R → E: Completely inactive. 1 Publication
Mutagenesisi169 – 1691R → K: The affinity for glycine and sarcosine decrease 50- and 63-fold, respectively, whereas the affinity for AdoMet changes only within 2-fold. Not able to catalyze the methylation of dimethylglycine. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Glycine/sarcosine N-methyltransferasePRO_0000412534Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ83WC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 1172S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18896.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83WC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKEKQVQD YGENPIEVRD SDHYQNEYIE GFVEKWDELI NWHARSSSEG
60 70 80 90 100
EFFIKTLKEH GAKRVLDAAT GTGFHSIRLI EAGFDVASVD GSVEMLVKAF
110 120 130 140 150
ENATRKDQIL RTVHSDWRQV TRHIQERFDA VICLGNSFTH LFSEEDRRKT
160 170 180 190 200
LAEFYSVLKH DGILILDQRN YDLILDEGFK SKHTYYYCGD NVKAEPEYVD
210 220 230 240 250
DGLARFRYEF PDQSVYHLNM FPLRKDYVRR LLHEVGFQDI TTYGDFQETY
260
HQDDPDFYIH VAKKD
Length:265
Mass (Da):31,213
Last modified:June 1, 2003 - v1
Checksum:iDD65C73F70E44536
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB094497 Genomic DNA. Translation: BAC56939.1.

Genome annotation databases

KEGGiag:BAC56939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB094497 Genomic DNA. Translation: BAC56939.1.

3D structure databases

ProteinModelPortaliQ83WC4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAC56939.

Phylogenomic databases

KOiK18896.

Enzyme and pathway databases

UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.
BioCyciMetaCyc:MONOMER-8562.
BRENDAi2.1.1.156. 383.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSMT_APHHA
AccessioniPrimary (citable) accession number: Q83WC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.