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Q83V26

- 3HAO_PSEFL

UniProt

Q83V26 - 3HAO_PSEFL

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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

nbaC

Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.1 PublicationUniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Binds 2 Fe2+ ions per subunit.1 Publication

Enzyme regulationi

Inhibited by Zn2+, Cu2+ and Cd2+.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481DioxygenUniRule annotation
Metal bindingi52 – 521Iron 1; catalyticCurated
Metal bindingi58 – 581Iron 1; catalyticUniRule annotation
Binding sitei58 – 581SubstrateUniRule annotation
Metal bindingi96 – 961Iron 1; catalyticCurated
Binding sitei100 – 1001SubstrateUniRule annotation
Binding sitei111 – 1111SubstrateUniRule annotation
Metal bindingi126 – 1261Iron 2UniRule annotation
Metal bindingi129 – 1291Iron 2UniRule annotation
Metal bindingi163 – 1631Iron 2UniRule annotation
Metal bindingi166 – 1661Iron 2UniRule annotation

GO - Molecular functioni

  1. 3-hydroxyanthranilate 3,4-dioxygenase activity Source: UniProtKB-HAMAP
  2. ferrous iron binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  4. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13359.
BRENDAi1.13.11.6. 5121.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:nbaCUniRule annotation
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521H → A: Loss of activity. 1 Publication
Mutagenesisi96 – 961H → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1851853-hydroxyanthranilate 3,4-dioxygenasePRO_0000245472Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ83V26.
SMRiQ83V26. Positions 2-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83V26-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMFTFGKPLN FQRWLDDHSD LLRPPVGNQQ VWQDSDFIVT VVGGPNFRTD
60 70 80 90 100
FHDDPMEEFF YQFKGNAYLN IMDRGQMDRV ELKEGDIFLL PPHLRHSPQR
110 120 130 140 150
PEAGSRCLVI ERQRPKGMLD GFEWYCLSCN GLVYRVDVQL NSIVTDLPPL
160 170 180
FDIFYGNVGL RKCPQCGQVH PGKAAIEAVA RGDQP
Length:185
Mass (Da):21,245
Last modified:June 1, 2003 - v1
Checksum:iAC932875CC9D3D7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB088043 Genomic DNA. Translation: BAC65311.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB088043 Genomic DNA. Translation: BAC65311.1 .

3D structure databases

ProteinModelPortali Q83V26.
SMRi Q83V26. Positions 2-175.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00330 .
BioCyci MetaCyc:MONOMER-13359.
BRENDAi 1.13.11.6. 5121.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_00825. 3_HAO.
InterProi IPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR15497. PTHR15497. 1 hit.
Pfami PF06052. 3-HAO. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR03037. anthran_nbaC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7."
    Muraki T., Taki M., Hasegawa Y., Iwaki H., Lau P.C.K.
    Appl. Environ. Microbiol. 69:1564-1572(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, FUNCTION, COFACTOR, SUBSTRATE SPECIFICITY, ENZYME REGULATION, MUTAGENESIS OF HIS-52 AND HIS-96.
    Strain: KU-7.

Entry informationi

Entry namei3HAO_PSEFL
AccessioniPrimary (citable) accession number: Q83V26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3