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Q83V26 (3HAO_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-hydroxyanthranilate 3,4-dioxygenase

EC=1.13.11.6
Alternative name(s):
3-hydroxyanthranilate oxygenase
Short name=3-HAO
3-hydroxyanthranilic acid dioxygenase
Short name=HAD
Gene names
Name:nbaC
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate. Ref.1

Catalytic activity

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde. HAMAP-Rule MF_00825

Cofactor

Binds 2 Fe2+ ions per subunit Probable. Ref.1

Enzyme regulation

Inhibited by Zn2+, Cu2+ and Cd2+. Ref.1

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3. HAMAP-Rule MF_00825

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00825

Sequence similarities

Belongs to the 3-HAO family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 1851853-hydroxyanthranilate 3,4-dioxygenase HAMAP-Rule MF_00825
PRO_0000245472

Sites

Metal binding521Iron 1; catalytic Probable
Metal binding581Iron 1; catalytic By similarity
Metal binding961Iron 1; catalytic Probable
Metal binding1261Iron 2 By similarity
Metal binding1291Iron 2 By similarity
Metal binding1631Iron 2 By similarity
Metal binding1661Iron 2 By similarity
Binding site481Dioxygen By similarity
Binding site581Substrate By similarity
Binding site1001Substrate By similarity
Binding site1111Substrate By similarity

Experimental info

Mutagenesis521H → A: Loss of activity. Ref.1
Mutagenesis961H → A: Loss of activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q83V26 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AC932875CC9D3D7D

FASTA18521,245
        10         20         30         40         50         60 
MMFTFGKPLN FQRWLDDHSD LLRPPVGNQQ VWQDSDFIVT VVGGPNFRTD FHDDPMEEFF 

        70         80         90        100        110        120 
YQFKGNAYLN IMDRGQMDRV ELKEGDIFLL PPHLRHSPQR PEAGSRCLVI ERQRPKGMLD 

       130        140        150        160        170        180 
GFEWYCLSCN GLVYRVDVQL NSIVTDLPPL FDIFYGNVGL RKCPQCGQVH PGKAAIEAVA 


RGDQP 

« Hide

References

[1]"Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7."
Muraki T., Taki M., Hasegawa Y., Iwaki H., Lau P.C.K.
Appl. Environ. Microbiol. 69:1564-1572(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, FUNCTION, COFACTOR, SUBSTRATE SPECIFICITY, ENZYME REGULATION, MUTAGENESIS OF HIS-52 AND HIS-96.
Strain: KU-7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB088043 Genomic DNA. Translation: BAC65311.1.

3D structure databases

ProteinModelPortalQ83V26.
SMRQ83V26. Positions 2-175.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13359.
BRENDA1.13.11.6. 5121.
UniPathwayUPA00253; UER00330.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
HAMAPMF_00825. 3_HAO.
InterProIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR15497. PTHR15497. 1 hit.
PfamPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMSSF51182. SSF51182. 1 hit.
TIGRFAMsTIGR03037. anthran_nbaC. 1 hit.
ProtoNetSearch...

Entry information

Entry name3HAO_PSEFL
AccessionPrimary (citable) accession number: Q83V26
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways