Q83V26 (3HAO_PSEFL) Reviewed, UniProtKB/Swiss-Prot
Last modified
October 19, 2011.
Version 42.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 3-hydroxyanthranilate 3,4-dioxygenase EC=1.13.11.6 Alternative name(s): 3-hydroxyanthranilate oxygenase Short name=3-HAO 3-hydroxyanthranilic acid dioxygenase Short name=HAD | ||
| Gene names |
| ||
| Organism | Pseudomonas fluorescens | ||
| Taxonomic identifier | 294 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 185 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate. Ref.1 |
| Catalytic activity | 3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde. HAMAP MF_00825 |
| Cofactor | Binds 2 Fe2+ ions per subunit Probable. Ref.1 |
| Enzyme regulation | Inhibited by Zn2+, Cu2+ and Cd2+. Ref.1 |
| Subunit structure | Homodimer By similarity. HAMAP MF_00825 |
| Sequence similarities | Belongs to the 3-HAO family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Iron Metal-binding |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular function | 3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 185 | 185 | 3-hydroxyanthranilate 3,4-dioxygenase HAMAP MF_00825 | PRO_0000245472 | |||||
Sites | |||||||||
| Metal binding | 52 | 1 | Iron 1; catalytic Probable | ||||||
| Metal binding | 58 | 1 | Iron 1; catalytic By similarity | ||||||
| Metal binding | 96 | 1 | Iron 1; catalytic Probable | ||||||
| Metal binding | 126 | 1 | Iron 2 By similarity | ||||||
| Metal binding | 129 | 1 | Iron 2 By similarity | ||||||
| Metal binding | 163 | 1 | Iron 2 By similarity | ||||||
| Metal binding | 166 | 1 | Iron 2 By similarity | ||||||
| Binding site | 48 | 1 | Dioxygen By similarity | ||||||
| Binding site | 58 | 1 | Substrate By similarity | ||||||
| Binding site | 100 | 1 | Substrate By similarity | ||||||
| Binding site | 111 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 52 | 1 | H → A: Loss of activity. Ref.1 | ||||||
| Mutagenesis | 96 | 1 | H → A: Loss of activity. Ref.1 | ||||||
Sequences
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References
| [1] | "Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7." Muraki T., Taki M., Hasegawa Y., Iwaki H., Lau P.C.K. Appl. Environ. Microbiol. 69:1564-1572(2003) [PubMed: 12620844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, FUNCTION, COFACTOR, SUBSTRATE SPECIFICITY, ENZYME REGULATION, MUTAGENESIS OF HIS-52 AND HIS-96. Strain: KU-7. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB088043 Genomic DNA. Translation: BAC65311.1. |
3D structure databases | |
| ProteinModelPortal | Q83V26. |
| SMR | Q83V26. Positions 2-175. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-13359. |
| BRENDA | 1.13.11.6. 5121. |
Family and domain databases | |
| HAMAP | MF_00825. 3_HAO. [Tree] |
| InterPro | IPR010329. 3hydroanth_dOase. IPR011051. Cupin_RmlC_type. IPR014710. RmlC-like_jellyroll. [Graphical view] |
| Gene3D | G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit. |
| PANTHER | PTHR15497. PTHR15497. 1 hit. |
| Pfam | PF06052. 3-HAO. 1 hit. [Graphical view] |
| SUPFAM | SSF51182. RmlC_like_cupin. 1 hit. |
| TIGRFAMs | TIGR03037. Anthran_nbaC. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | 3HAO_PSEFL | ||||||||
| Accession | Primary (citable) accession number: Q83V26 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |