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Q83V26

- 3HAO_PSEFL

UniProt

Q83V26 - 3HAO_PSEFL

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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene
nbaC
Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.1 Publication

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Binds 2 Fe2+ ions per subunit Inferred.1 Publication

Enzyme regulationi

Inhibited by Zn2+, Cu2+ and Cd2+.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481Dioxygen By similarity
Metal bindingi52 – 521Iron 1; catalytic Inferred
Metal bindingi58 – 581Iron 1; catalytic By similarity
Binding sitei58 – 581Substrate By similarity
Metal bindingi96 – 961Iron 1; catalytic Inferred
Binding sitei100 – 1001Substrate By similarity
Binding sitei111 – 1111Substrate By similarity
Metal bindingi126 – 1261Iron 2 By similarity
Metal bindingi129 – 1291Iron 2 By similarity
Metal bindingi163 – 1631Iron 2 By similarity
Metal bindingi166 – 1661Iron 2 By similarity

GO - Molecular functioni

  1. 3-hydroxyanthranilate 3,4-dioxygenase activity Source: UniProtKB-HAMAP
  2. ferrous iron binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  4. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13359.
BRENDAi1.13.11.6. 5121.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenase (EC:1.13.11.6)
Alternative name(s):
3-hydroxyanthranilate oxygenase
Short name:
3-HAO
3-hydroxyanthranilic acid dioxygenase
Short name:
HAD
Gene namesi
Name:nbaC
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521H → A: Loss of activity. 1 Publication
Mutagenesisi96 – 961H → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1851853-hydroxyanthranilate 3,4-dioxygenaseUniRule annotationPRO_0000245472Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ83V26.
SMRiQ83V26. Positions 2-175.

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-HAO family.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83V26-1 [UniParc]FASTAAdd to Basket

« Hide

MMFTFGKPLN FQRWLDDHSD LLRPPVGNQQ VWQDSDFIVT VVGGPNFRTD    50
FHDDPMEEFF YQFKGNAYLN IMDRGQMDRV ELKEGDIFLL PPHLRHSPQR 100
PEAGSRCLVI ERQRPKGMLD GFEWYCLSCN GLVYRVDVQL NSIVTDLPPL 150
FDIFYGNVGL RKCPQCGQVH PGKAAIEAVA RGDQP 185
Length:185
Mass (Da):21,245
Last modified:June 1, 2003 - v1
Checksum:iAC932875CC9D3D7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB088043 Genomic DNA. Translation: BAC65311.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB088043 Genomic DNA. Translation: BAC65311.1 .

3D structure databases

ProteinModelPortali Q83V26.
SMRi Q83V26. Positions 2-175.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00330 .
BioCyci MetaCyc:MONOMER-13359.
BRENDAi 1.13.11.6. 5121.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_00825. 3_HAO.
InterProi IPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR15497. PTHR15497. 1 hit.
Pfami PF06052. 3-HAO. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR03037. anthran_nbaC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7."
    Muraki T., Taki M., Hasegawa Y., Iwaki H., Lau P.C.K.
    Appl. Environ. Microbiol. 69:1564-1572(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, FUNCTION, COFACTOR, SUBSTRATE SPECIFICITY, ENZYME REGULATION, MUTAGENESIS OF HIS-52 AND HIS-96.
    Strain: KU-7.

Entry informationi

Entry namei3HAO_PSEFL
AccessioniPrimary (citable) accession number: Q83V26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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