Reviewed, UniProtKB/Swiss-Prot Q83RW9 (MBHS_SHIFL)

Names and origin

Protein names

Recommended name:
    Hydrogenase-1 small chain
      Short name=HYD1
    EC=1.12.99.6
Alternative name(s):
    Membrane-bound hydrogenase 1 small subunit
    NiFe hydrogenase

Gene names

Name:	hyaA
Ordered Locus Names:	SF0973, S1040

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This is one of three S.flexneri hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth By similarity.
Catalytic activity	H(2) + A = AH(2).
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Subcellular location	Cell inner membrane; Single-pass type I membrane proteinBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane Periplasm
Domain	Signal Transmembrane
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 45	45	Tat-type signal Potential
	Chain	46 – 372	327	Hydrogenase-1 small chain		PRO_0000013429
Regions
	Topological domain	46 – 325	280	Periplasmic Potential
	Transmembrane	326 – 346	21	Potential
	Topological domain	347 – 372	26	Cytoplasmic Potential
Sites
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	160	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	194	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	232	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	235	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	260	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	266	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	275	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	294	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	297	1	Iron-sulfur 3 (3Fe-4S) By similarity
Experimental info
	Sequence conflict	347	1	S → A in AAP16487. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q83RW9-1 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: 4AC0CD86220D2826	FASTA	372	40,697
	10 20 30 40 50 60 MNNEETFYQA MRRQGVTRRS FLKYCSLAAT SLGLGAGMAP KIAWALENKP RIPVVWIHGL 70 80 90 100 110 120 ECTCCTESFI RSAHPLAKDV ILSLISLDYD DTLMAAAGTQ AEEVFEDIIT QYNGKYILAV 130 140 150 160 170 180 EGNPPLGEQG MFCISSGRPF IEKLKRAAAG ASAIIAWGTC ASWGCVQAAR PNPTQATPID 190 200 210 220 230 240 KVITDKPIIK VPGCPPIPDV MSAIITYMVT FDRLPDVDRM GRPLMFYGQR IHDKCYRRAH 250 260 270 280 290 300 FDAGEFVQSW DDDAARKGYC LYKMGCKGPT TYNACSSTRW NDGVSFPIQS GHGCLGCAEN 310 320 330 340 350 360 GFWDRGSFYS RVVDIPQMGT HSTADTVGLT ALGVVAAAVG VHAVASSVDQ RRRHNQQPTE 370 TEHQPGNEDK QA

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN42601.1. Different initiation. AE014073 Genomic DNA. Translation: AAP16487.1.
RefSeq	NP_706894.2. NP_836681.1.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Genome annotation databases
GeneID	1023950. 1077442.
GenomeReviews	Gene locus S1040 in contig AE014073_GR. Gene locus SF0973 in contig AE005674_GR.
KEGG	sfl:SF0973. sfx:S1040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q83RW9.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN42601.1-MON.
Family and domain databases
InterPro	IPR001821. NiFe_hyd_ssu. IPR013634. NiFe_hyd_ssu_N. IPR006137. OxRdtase_q6. IPR006311. Tat. [Graphical view]
Pfam	PF08425. NiFe_dehyd_N. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_SHIFL

Accession

Primary (citable) accession number: Q83RW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	July 11, 2003
Last modified:	November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)