Q83RR9 (NAGK_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	SF1123, S1203

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity. HAMAP-Rule MF_01271
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP-Rule MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_01271
Sequence similarities	Belongs to the ROK (NagC/XylR) family. NagK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	N-acetyl-D-glucosamine kinase HAMAP-Rule MF_01271		PRO_0000270117
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential
Sites
	Metal binding	157	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	184	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83RR9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 65572B2CCFEBFBA5	FASTA	303	32,987
	10 20 30 40 50 60 MYYGFDIGGT KIALGVFDSG RQLQWEKRVP TPRDSYDAFL DAVCELVAEA DQRFGCKGSV 70 80 90 100 110 120 GIGIPGMPET EDGKLYAANV PAASGKPLRA DLSARLDRDV RLDNDANCFA LSEAWDDEFT 130 140 150 160 170 180 QYPLVMGLIL GTGVGGGLVF NGKPITGKSY ITGEFGHMRL PVDALTMMGL DFPLRRCGCG 190 200 210 220 230 240 QHGCIENYLS GRGFAWLYQH YYHQPLQAPE IIALYDQGDE QARAHVERYL DLLAVSLGNI 250 260 270 280 290 300 LTIVDPDLVV IGGGLSNFPA ITTQLADRLP CHLLPVARVP RIERARHGDA GGMRGAAFLH LTD

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN42741.1. AE014073 Genomic DNA. Translation: AAP16630.1.
RefSeq	NP_707034.1. NC_004337.2. NP_836823.1. NC_004741.1.
3D structure databases
HSSP	HSSP built from PDB template 2AP1 based on UniProtKB Q8ZPZ9.
ProteinModelPortal	Q83RR9.
SMR	Q83RR9. Positions 1-303.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF1123.
Proteomic databases
PaxDb	Q83RR9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN42741; AAN42741; SF1123. AAP16630; AAP16630; S1203.
GeneID	1024059. 1076442.
KEGG	sfl:SF1123. sfx:S1203.
PATRIC	18703616. VBIShiFle31049_1313.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1940.
HOGENOM	HOG000150087.
KO	K00884.
OMA	FGHIRLP.
ProtClustDB	PRK13310.
Enzyme and pathway databases
UniPathway	UPA00544.
Family and domain databases
HAMAP	MF_01271. GlcNAc_kinase.
InterPro	IPR023505. N-acetyl-D-glucosamine_kinase. IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_SHIFL

Accession

Primary (citable) accession number: Q83RR9
Secondary accession number(s): Q7C210

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program