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Q83RN5 (NARH_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Respiratory nitrate reductase 1 beta chain
EC=1.7.99.4
Gene names
Name:narH
Ordered Locus Names:SF1228, S1312
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The nitrate reductase enzyme complex allows S.flexneri to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit By similarity.

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 3 4Fe-4S clusters per subunit By similarity.

Binds 1 3Fe-4S cluster per subunit By similarity.

Subunit structure

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Contains 3 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Respiratory nitrate reductase 1 beta chain
PRO_0000096721

Regions

Domain7 – 35294Fe-4S ferredoxin-type 1
Domain175 – 206324Fe-4S ferredoxin-type 2
Domain208 – 237304Fe-4S ferredoxin-type 3

Sites

Metal binding161Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding191Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding221Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding261Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1841Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1871Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1921Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1961Iron-sulfur 4 (3Fe-4S) By similarity
Metal binding2171Iron-sulfur 4 (3Fe-4S) By similarity
Metal binding2231Iron-sulfur 4 (3Fe-4S) By similarity
Metal binding2271Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding2441Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2471Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2591Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2631Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict4021I → V in AAP16727. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q83RN5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: F8AC747E1C0973DB

FASTA51258,080
        10         20         30         40         50         60 
MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TSREGVEYAW FNNVETKPGQ GFPTDWENQE 

        70         80         90        100        110        120 
KYKGGWIRKI NGKLQPRMGN RAMLLGKIFA NPHLPGIDDY YEPFDFDYQN LHTAPEGSKS 

       130        140        150        160        170        180 
QPIARPRSLI TGERMAKIEK GPNWEDDLGG EFDKLAKDKN FDNIQKAMYS QFENTFMMYL 

       190        200        210        220        230        240 
PRLCEHCLNP ACVATCPSGA IYKREEDGIV LIDQDKCRGW RMCITGCPYK KIYFNWKSGK 

       250        260        270        280        290        300 
SEKCIFCYPR IEAGQPTVCS ETCVGRIRYL GVLLYDADAI ERAASTENEK DLYQRQLDVF 

       310        320        330        340        350        360 
LDPNDPKVIE QAIKDGIPLS VIEAAQQSPV YKMAMEWKLA LPLHPEYRTL PMVWYVPPLS 

       370        380        390        400        410        420 
PIQSAADAGE LGSNGILPDV ESLRIPVQYL ANLLTAGDTK PILRALKRML AMRHYKRAET 

       430        440        450        460        470        480 
VDGKVDTRAL EEVGLTEAQA QEMYRYLAIA NYEDRFVVPS SHRELAREAF PEKNGCGFTF 

       490        500        510 
GDGCHGSDTK FNLFNSRRID AIDVTSKTEP HP

« Hide

References

[1]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[2]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005674 Genomic DNA. Translation: AAN42841.1.
AE014073 Genomic DNA. Translation: AAP16727.1.
RefSeqNP_707134.1. NC_004337.2.
NP_836920.1. NC_004741.1.

3D structure databases

ProteinModelPortalQ83RN5.
SMRQ83RN5. Positions 1-509.
ModBaseSearch...

Protein-protein interaction databases

STRING198214.SF1228.

Proteomic databases

PaxDbQ83RN5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN42841; AAN42841; SF1228.
AAP16727; AAP16727; S1312.
GeneID1024183.
1077691.
KEGGsfl:SF1228.
sfx:S1312.
PATRIC18703866. VBIShiFle31049_1438.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1140.
HOGENOMHOG000237353.
KOK00371.
OMAEYAENAF.
ProtClustDBCLSK868287.

Family and domain databases

InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamPF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsTIGR01660. narH. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. False negative.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARH_SHIFL
AccessionPrimary (citable) accession number: Q83RN5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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