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Protein

Respiratory nitrate reductase 1 beta chain

Gene

narH

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The nitrate reductase enzyme complex allows S.flexneri to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit (By similarity).By similarity

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
  • [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi19 – 191Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi22 – 221Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi26 – 261Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi184 – 1841Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi187 – 1871Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi192 – 1921Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi196 – 1961Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi217 – 2171Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi223 – 2231Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi227 – 2271Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi244 – 2441Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi247 – 2471Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi259 – 2591Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi263 – 2631Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase 1 beta chain (EC:1.7.99.4)
Gene namesi
Name:narH
Ordered Locus Names:SF1228, S1312
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000002673 Componenti: Chromosome UP000001006 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Respiratory nitrate reductase 1 beta chainPRO_0000096721Add
BLAST

Proteomic databases

PaxDbiQ83RN5.

Interactioni

Subunit structurei

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane (By similarity).By similarity

Protein-protein interaction databases

STRINGi198214.SF1228.

Structurei

3D structure databases

ProteinModelPortaliQ83RN5.
SMRiQ83RN5. Positions 1-509.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 35294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini175 – 206324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini208 – 237304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1140.
HOGENOMiHOG000237353.
KOiK00371.
OrthoDBiEOG6X3W4C.

Family and domain databases

Gene3Di1.10.3650.10. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01660. narH. 1 hit.
PROSITEiPS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83RN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TSREGVEYAW FNNVETKPGQ
60 70 80 90 100
GFPTDWENQE KYKGGWIRKI NGKLQPRMGN RAMLLGKIFA NPHLPGIDDY
110 120 130 140 150
YEPFDFDYQN LHTAPEGSKS QPIARPRSLI TGERMAKIEK GPNWEDDLGG
160 170 180 190 200
EFDKLAKDKN FDNIQKAMYS QFENTFMMYL PRLCEHCLNP ACVATCPSGA
210 220 230 240 250
IYKREEDGIV LIDQDKCRGW RMCITGCPYK KIYFNWKSGK SEKCIFCYPR
260 270 280 290 300
IEAGQPTVCS ETCVGRIRYL GVLLYDADAI ERAASTENEK DLYQRQLDVF
310 320 330 340 350
LDPNDPKVIE QAIKDGIPLS VIEAAQQSPV YKMAMEWKLA LPLHPEYRTL
360 370 380 390 400
PMVWYVPPLS PIQSAADAGE LGSNGILPDV ESLRIPVQYL ANLLTAGDTK
410 420 430 440 450
PILRALKRML AMRHYKRAET VDGKVDTRAL EEVGLTEAQA QEMYRYLAIA
460 470 480 490 500
NYEDRFVVPS SHRELAREAF PEKNGCGFTF GDGCHGSDTK FNLFNSRRID
510
AIDVTSKTEP HP
Length:512
Mass (Da):58,080
Last modified:June 1, 2003 - v1
Checksum:iF8AC747E1C0973DB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti402 – 4021I → V in AAP16727 (PubMed:12704152).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN42841.1.
AE014073 Genomic DNA. Translation: AAP16727.1.
RefSeqiNP_707134.1. NC_004337.2.

Genome annotation databases

EnsemblBacteriaiAAN42841; AAN42841; SF1228.
AAP16727; AAP16727; S1312.
GeneIDi1024183.
KEGGisfl:SF1228.
sfx:S1312.
PATRICi18703866. VBIShiFle31049_1438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN42841.1.
AE014073 Genomic DNA. Translation: AAP16727.1.
RefSeqiNP_707134.1. NC_004337.2.

3D structure databases

ProteinModelPortaliQ83RN5.
SMRiQ83RN5. Positions 1-509.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF1228.

Proteomic databases

PaxDbiQ83RN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN42841; AAN42841; SF1228.
AAP16727; AAP16727; S1312.
GeneIDi1024183.
KEGGisfl:SF1228.
sfx:S1312.
PATRICi18703866. VBIShiFle31049_1438.

Phylogenomic databases

eggNOGiCOG1140.
HOGENOMiHOG000237353.
KOiK00371.
OrthoDBiEOG6X3W4C.

Family and domain databases

Gene3Di1.10.3650.10. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01660. narH. 1 hit.
PROSITEiPS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiNARH_SHIFL
AccessioniPrimary (citable) accession number: Q83RN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.