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Q83RC9 (FOLM_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase FolM

Short name=DHFR
EC=1.5.1.3
Gene names
Name:folM
Ordered Locus Names:SF1627, S1759
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of dihydrofolate to tetrahydrofolate By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processone-carbon metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondihydrofolate reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Dihydrofolate reductase FolM
PRO_0000339401

Sites

Active site1521Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83RC9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 1A227307B826086A

FASTA24026,330
        10         20         30         40         50         60 
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA GAQCIQADFS 

        70         80         90        100        110        120 
TNDGVMAFAD EVLKTTHGLR AILHNASAWI AEKPGAPLAD VLACMMQIHV NTPYLLNHAL 

       130        140        150        160        170        180 
ERLLRGHGHA ASDIIHFTDY VVERGSDKHV AYAASKAALD NMTRSFARKL APEVKVNSIA 

       190        200        210        220        230        240 
PSLILFNEHD DAEYRQQALN KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR 

« Hide

References

[1]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[2]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005674 Genomic DNA. Translation: AAN43210.1.
AE014073 Genomic DNA. Translation: AAP17098.1.
RefSeqNP_707503.1. NC_004337.2.
NP_837291.1. NC_004741.1.

3D structure databases

ProteinModelPortalQ83RC9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198214.SF1627.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN43210; AAN43210; SF1627.
AAP17098; AAP17098; S1759.
GeneID1024813.
1078448.
KEGGsfl:SF1627.
sfx:S1759.
PATRIC18704899. VBIShiFle31049_1948.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1028.
KOK13938.
OMAMKIAPGE.
OrthoDBEOG6WDSG3.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFOLM_SHIFL
AccessionPrimary (citable) accession number: Q83RC9
Secondary accession number(s): Q7C1H7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families