Q83R14 (COBT_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	SF2059, S2169

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB) By similarity. HAMAP-Rule MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP-Rule MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP-Rule MF_00230
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP-Rule MF_00230		PRO_0000167071
Sites
	Active site	318	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83R14 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 337ABE66B7B6443F	FASTA	359	36,880
	10 20 30 40 50 60 MQIIADLLNT IPAINSAAMS RAQRHVDGLF KPVGSLGKLE ALAIQLAGMP GVNGIPHVGK 70 80 90 100 110 120 KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC VLAAQAGANV HVIDVGIDTA 130 140 150 160 170 180 EPIPGLINMR VARGSGNIAS APAMSRRQAE KLLLDVICYT RELAKNGVTL FGVGELGMAN 190 200 210 220 230 240 TTPAAAIVST ITGRDPEEVV GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG 250 260 270 280 290 300 GFDLVGIAGV MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA 310 320 330 340 350 LSHLGLEPYL NMDMRLGEGS GAALAMSIIE AACAIYNNMG ELAASNIVLP GNTTSDLNS

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN43600.1. AE014073 Genomic DNA. Translation: AAP17423.1.
RefSeq	NP_707893.1. NC_004337.2. NP_837614.1. NC_004741.1.
3D structure databases
ProteinModelPortal	Q83R14.
SMR	Q83R14. Positions 7-350.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF2059.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN43600; AAN43600; SF2059. AAP17423; AAP17423; S2169.
GeneID	1025890. 1078475.
KEGG	sfl:SF2059. sfx:S2169.
PATRIC	18705851. VBIShiFle31049_2404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2038.
HOGENOM	HOG000263499.
KO	K00768.
OMA	FICSVAA.
ProtClustDB	PRK00105.
Enzyme and pathway databases
UniPathway	UPA00061; UER00516.
Family and domain databases
Gene3D	1.10.1610.10. 1 hit.
HAMAP	MF_00230. CobT.
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. cobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_SHIFL

Accession

Primary (citable) accession number: Q83R14

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program