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Protein

Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

Gene

cobT

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).UniRule annotation

Catalytic activityi

Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei318 – 3181Proton acceptorUniRule annotation

GO - Molecular functioni

  1. cobalamin-transporting ATPase activity Source: UniProtKB-HAMAP
  2. nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-KW
  2. nucleoside biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00061; UER00516.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferaseUniRule annotation (EC:2.4.2.21UniRule annotation)
Short name:
NN:DBI PRTUniRule annotation
Alternative name(s):
N(1)-alpha-phosphoribosyltransferaseUniRule annotation
Gene namesi
Name:cobTUniRule annotation
Ordered Locus Names:SF2059, S2169
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferasePRO_0000167071Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198214.SF2059.

Structurei

3D structure databases

ProteinModelPortaliQ83R14.
SMRiQ83R14. Positions 7-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobT family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2038.
HOGENOMiHOG000263499.
KOiK00768.
OMAiPHWALGR.
OrthoDBiEOG6HF61K.

Family and domain databases

Gene3Di1.10.1610.10. 1 hit.
HAMAPiMF_00230. CobT.
InterProiIPR003200. Nict_dMeBzImd_PRibTrfase-like.
IPR023195. Nict_dMeBzImd_PRibTrfase_N.
IPR017846. Nict_dMeBzImd_PRibTrfase_pro.
[Graphical view]
PfamiPF02277. DBI_PRT. 1 hit.
[Graphical view]
SUPFAMiSSF52733. SSF52733. 1 hit.
TIGRFAMsiTIGR03160. cobT_DBIPRT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83R14-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQIIADLLNT IPAINSAAMS RAQRHVDGLF KPVGSLGKLE ALAIQLAGMP
60 70 80 90 100
GVNGIPHVGK KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC
110 120 130 140 150
VLAAQAGANV HVIDVGIDTA EPIPGLINMR VARGSGNIAS APAMSRRQAE
160 170 180 190 200
KLLLDVICYT RELAKNGVTL FGVGELGMAN TTPAAAIVST ITGRDPEEVV
210 220 230 240 250
GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG GFDLVGIAGV
260 270 280 290 300
MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA
310 320 330 340 350
LSHLGLEPYL NMDMRLGEGS GAALAMSIIE AACAIYNNMG ELAASNIVLP

GNTTSDLNS
Length:359
Mass (Da):36,880
Last modified:June 1, 2003 - v1
Checksum:i337ABE66B7B6443F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN43600.1.
AE014073 Genomic DNA. Translation: AAP17423.1.
RefSeqiNP_707893.1. NC_004337.2.
NP_837614.1. NC_004741.1.
WP_001165576.1. NZ_KL503861.1.

Genome annotation databases

EnsemblBacteriaiAAN43600; AAN43600; SF2059.
AAP17423; AAP17423; S2169.
GeneIDi1025890.
1078475.
KEGGisfl:SF2059.
sfx:S2169.
PATRICi18705851. VBIShiFle31049_2404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN43600.1.
AE014073 Genomic DNA. Translation: AAP17423.1.
RefSeqiNP_707893.1. NC_004337.2.
NP_837614.1. NC_004741.1.
WP_001165576.1. NZ_KL503861.1.

3D structure databases

ProteinModelPortaliQ83R14.
SMRiQ83R14. Positions 7-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF2059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN43600; AAN43600; SF2059.
AAP17423; AAP17423; S2169.
GeneIDi1025890.
1078475.
KEGGisfl:SF2059.
sfx:S2169.
PATRICi18705851. VBIShiFle31049_2404.

Phylogenomic databases

eggNOGiCOG2038.
HOGENOMiHOG000263499.
KOiK00768.
OMAiPHWALGR.
OrthoDBiEOG6HF61K.

Enzyme and pathway databases

UniPathwayiUPA00061; UER00516.

Family and domain databases

Gene3Di1.10.1610.10. 1 hit.
HAMAPiMF_00230. CobT.
InterProiIPR003200. Nict_dMeBzImd_PRibTrfase-like.
IPR023195. Nict_dMeBzImd_PRibTrfase_N.
IPR017846. Nict_dMeBzImd_PRibTrfase_pro.
[Graphical view]
PfamiPF02277. DBI_PRT. 1 hit.
[Graphical view]
SUPFAMiSSF52733. SSF52733. 1 hit.
TIGRFAMsiTIGR03160. cobT_DBIPRT. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiCOBT_SHIFL
AccessioniPrimary (citable) accession number: Q83R14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.