Q83QL4 (PUR5_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	SF2543, S2692

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.
Sequence caution	The sequence AAN44044.1 differs from that shown. Reason: Erroneous initiation. The sequence AAP17854.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 345	344	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000148244

Sequences

	Sequence		Length	Mass (Da)
	Q83QL4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 2DBC0975E81815D7	FASTA	345	36,873
	10 20 30 40 50 60 MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA LPQKYREPVL 70 80 90 100 110 120 VSGTDGVGTK LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA EPLFFLDYYA TGKLDVDTAS 130 140 150 160 170 180 AVISGIAEGC LQSGCSLVGG ETAEMPGMYH GEDYDVAGFC VGVVEKSEII DGSKVSDGDV 190 200 210 220 230 240 LIALGSSGPH SNGYSLVRKI LEVSGCDPQT TELDGKPLAD HLLAPTRIYV KSVLELIEKV 250 260 270 280 290 300 DVHAIAHLTG GGFWENIPRV LPDNTQAVID ESSWQWPEVF NWLQTAGNVE RHEMYRTFNC 310 320 330 340 GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN44044.1. Different initiation. AE014073 Genomic DNA. Translation: AAP17854.1. Different initiation.
RefSeq	NP_708337.1. NC_004337.2. NP_838044.1. NC_004741.1.
3D structure databases
ProteinModelPortal	Q83QL4.
SMR	Q83QL4. Positions 5-345.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF2543.
Proteomic databases
PaxDb	Q83QL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN44044; AAN44044; SF2543. AAP17854; AAP17854; S2692.
GeneID	1024382. 1078964.
KEGG	sfl:SF2543. sfx:S2692.
PATRIC	18707020. VBIShiFle31049_2978.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229091.
KO	K01933.
ProtClustDB	PRK05385.
Enzyme and pathway databases
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_SHIFL

Accession

Primary (citable) accession number: Q83QL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program