Q83P01 (TYPH_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine phosphorylase
EC=2.4.2.4

Alternative name(s):
TdRPase

Gene names

Name:	deoA
Ordered Locus Names:	SF4414, S4685

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis By similarity. HAMAP-Rule MF_01628
Catalytic activity	Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01628
Pathway	Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. HAMAP-Rule MF_01628
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyrimidine nucleobase metabolic process Inferred from electronic annotation. Source: InterPro thymidine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	phosphorylase activity Inferred from electronic annotation. Source: InterPro pyrimidine-nucleoside phosphorylase activity Inferred from electronic annotation. Source: InterPro thymidine phosphorylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Thymidine phosphorylase HAMAP-Rule MF_01628		PRO_0000059068

Sequences

	Sequence		Length	Mass (Da)
	Q83P01 [UniParc]. Last modified July 5, 2005. Version 4. Checksum: 07BDF83C92737ACA	FASTA	440	47,142
	10 20 30 40 50 60 MFLAQEIIRK KRDGHALSDE EIRFFINGIR DNTISEGQIA ALAMTIFFHD MTMPERVSLT 70 80 90 100 110 120 MAMRDSGTVL DWKSLHLNGP IVDKHSTGGV GDVTSLMLGP MVAACGGYIP MISGRGLGHT 130 140 150 160 170 180 GGTLDKLESI PGFDIFPDDN RFREIIKDVG VAIIGQTSSL APADKRFYAT RDITATVDSI 190 200 210 220 230 240 PLITASILAK KLAEGLDALV MDVKVGSGAF MPTYELSEAL AEAIVGVANG AGVRTTALLT 250 260 270 280 290 300 DMNQVLASSA GNAVEVREAV QFLTGEYRNP RLFDVTMALC VEMLISGKLA KDDAEARAKL 310 320 330 340 350 360 QAVLDNGKAA EVFGRMVAAQ KGPTDFVENY AKYLPTAMLT KAVYADTEGF VSEMDTRALG 370 380 390 400 410 420 MAVVAMGGGR RQASDTIDYS VGFTDMARLG DQVDGQRPLA VILAKDENSW QEAAKAVKAA 430 440 IKLADNAPES TPTVYRRISE

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN45829.2. AE014073 Genomic DNA. Translation: AAP19603.1.
RefSeq	NP_710122.2. NC_004337.2. NP_839791.1. NC_004741.1.
3D structure databases
ProteinModelPortal	Q83P01.
SMR	Q83P01. Positions 1-440.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF4414.
Proteomic databases
PaxDb	Q83P01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN45829; AAN45829; SF4414. AAP19603; AAP19603; S4685.
GeneID	1025453. 1080883.
KEGG	sfl:SF4414. sfx:S4685.
PATRIC	18711432. VBIShiFle31049_5111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0213.
HOGENOM	HOG000047313.
KO	K00758.
ProtClustDB	PRK05820.
Enzyme and pathway databases
UniPathway	UPA00578; UER00638.
Family and domain databases
Gene3D	3.40.1030.10. 1 hit. 3.90.1170.30. 1 hit.
HAMAP	MF_01628. Thymid_phosp.
InterPro	IPR000312. Glycosyl_Trfase_fam3. IPR017459. Glycosyl_Trfase_fam3_N_dom. IPR013102. PYNP_C. IPR018090. Pyrmidine_PPas_bac/euk. IPR000053. Pyrmidine_PPase. IPR017872. Pyrmidine_PPase_CS. IPR013465. Thymidine_Pase. [Graphical view]
PANTHER	PTHR10515. PTHR10515. 1 hit.
Pfam	PF02885. Glycos_trans_3N. 1 hit. PF00591. Glycos_transf_3. 1 hit. PF07831. PYNP_C. 1 hit. [Graphical view]
PIRSF	PIRSF000478. TP_PyNP. 1 hit.
SMART	SM00941. PYNP_C. 1 hit. [Graphical view]
SUPFAM	SSF47648. Glyco_trans_3. 1 hit. SSF52418. Glyco_trans_3. 1 hit. SSF54680. PYNP_C. 1 hit.
TIGRFAMs	TIGR02643. T_phosphoryl. 1 hit. TIGR02644. Y_phosphoryl. 1 hit.
PROSITE	PS00647. THYMID_PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYPH_SHIFL

Accession

Primary (citable) accession number: Q83P01
Secondary accession number(s): Q7UAI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 77 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program