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Q83NN8

- LIPA_TROW8

UniProt

Q83NN8 - LIPA_TROW8

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Protein

Lipoyl synthase

Gene
lipA, TW301
Organism
Tropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:TW301
OrganismiTropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Taxonomic identifieri218496 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000001413: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Lipoyl synthaseUniRule annotationPRO_0000102375Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi218496.TW301.

Structurei

3D structure databases

ProteinModelPortaliQ83NN8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83NN8-1 [UniParc]FASTAAdd to Basket

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MNDSGNSSKV NVRPPSAGLG APSPGKRKML RLEVRNSQVP IERKPSWIRA    50
RATIGTEYRK VQERVKKQNL RTVCQEAGCP NIYECWEDRE ATFLIGGSQC 100
TRRCDFCQID TGKPAELDLD EPKRVGQSVA QMKLRYATVT GVARDDLPDG 150
GVWLYAETIR EIHKQCPGSG VEILIPDFNG KPELLQQIFE AQPEVYAHNI 200
ETVPRIFRRI RPAFRYDRSL DVISQGQKAG MITKSNLILG MGETGEEVTQ 250
ALRDLKSAGC DIVTITQYLR PSPRHLPVAR WVKPQEFIEY KEQAKEIGFS 300
GVLAGPLVRS SYRAGKLWAQ SVKAKMVEIP ERVRKLINEI EMQETSFRQA 350
V 351
Length:351
Mass (Da):39,596
Last modified:June 1, 2003 - v1
Checksum:i998C3989CADF3E18
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX251411 Genomic DNA. Translation: CAD66975.1.
RefSeqiNP_789237.1. NC_004551.1.
WP_011096255.1. NC_004551.1.

Genome annotation databases

EnsemblBacteriaiCAD66975; CAD66975; TW301.
GeneIDi1064590.
KEGGitws:TW301.
PATRICi23999059. VBITroWhi42739_0365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX251411 Genomic DNA. Translation: CAD66975.1 .
RefSeqi NP_789237.1. NC_004551.1.
WP_011096255.1. NC_004551.1.

3D structure databases

ProteinModelPortali Q83NN8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218496.TW301.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD66975 ; CAD66975 ; TW301 .
GeneIDi 1064590.
KEGGi tws:TW301.
PATRICi 23999059. VBITroWhi42739_0365.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW08/27.

Entry informationi

Entry nameiLIPA_TROW8
AccessioniPrimary (citable) accession number: Q83NN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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