Q83MU9 (LIPA_TROWT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase
EC=2.8.1.8

Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA

Gene names

Name:	lipA
Ordered Locus Names:	TWT_464

Organism

Tropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

203267 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › unclassified Micrococcineae › Tropheryma › Tropheryma whipplei

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00206.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.
Sequence caution	The sequence AAO44561.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Lipoyl synthase HAMAP-Rule MF_00206		PRO_0000102376
Sites
	Metal binding	74	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	85	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	100	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	104	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	107	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83MU9 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 998C3989CADF3E18	FASTA	351	39,596
	10 20 30 40 50 60 MNDSGNSSKV NVRPPSAGLG APSPGKRKML RLEVRNSQVP IERKPSWIRA RATIGTEYRK 70 80 90 100 110 120 VQERVKKQNL RTVCQEAGCP NIYECWEDRE ATFLIGGSQC TRRCDFCQID TGKPAELDLD 130 140 150 160 170 180 EPKRVGQSVA QMKLRYATVT GVARDDLPDG GVWLYAETIR EIHKQCPGSG VEILIPDFNG 190 200 210 220 230 240 KPELLQQIFE AQPEVYAHNI ETVPRIFRRI RPAFRYDRSL DVISQGQKAG MITKSNLILG 250 260 270 280 290 300 MGETGEEVTQ ALRDLKSAGC DIVTITQYLR PSPRHLPVAR WVKPQEFIEY KEQAKEIGFS 310 320 330 340 350 GVLAGPLVRS SYRAGKLWAQ SVKAKMVEIP ERVRKLINEI EMQETSFRQA V

References

[1]

"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome." Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M. Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Twist.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014184 Genomic DNA. Translation: AAO44561.1. Different initiation.
RefSeq	NP_787592.2. NC_004572.3.
3D structure databases
ProteinModelPortal	Q83MU9.
ModBase	Search...
Protein-protein interaction databases
STRING	203267.TWT464.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO44561; AAO44561; TWT_464.
GeneID	1089343.
KEGG	twh:TWT464.
PATRIC	23997471. VBITroWhi72342_0606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0320.
KO	K03644.
OMA	EEYVTPE.
ProtClustDB	PRK05481.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_TROWT

Accession

Primary (citable) accession number: Q83MU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program