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Protein

Lipoyl synthase

Gene

lipA

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi85Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi100Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi104Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi107Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:TWT_464
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesTropheryma
Proteomesi
  • UP000002200 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023761 – 351Lipoyl synthaseAdd BLAST351

Interactioni

Protein-protein interaction databases

STRINGi203267.TWT464

Structurei

3D structure databases

ProteinModelPortaliQ83MU9
SMRiQ83MU9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q83MU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDSGNSSKV NVRPPSAGLG APSPGKRKML RLEVRNSQVP IERKPSWIRA
60 70 80 90 100
RATIGTEYRK VQERVKKQNL RTVCQEAGCP NIYECWEDRE ATFLIGGSQC
110 120 130 140 150
TRRCDFCQID TGKPAELDLD EPKRVGQSVA QMKLRYATVT GVARDDLPDG
160 170 180 190 200
GVWLYAETIR EIHKQCPGSG VEILIPDFNG KPELLQQIFE AQPEVYAHNI
210 220 230 240 250
ETVPRIFRRI RPAFRYDRSL DVISQGQKAG MITKSNLILG MGETGEEVTQ
260 270 280 290 300
ALRDLKSAGC DIVTITQYLR PSPRHLPVAR WVKPQEFIEY KEQAKEIGFS
310 320 330 340 350
GVLAGPLVRS SYRAGKLWAQ SVKAKMVEIP ERVRKLINEI EMQETSFRQA

V
Length:351
Mass (Da):39,596
Last modified:May 10, 2004 - v2
Checksum:i998C3989CADF3E18
GO

Sequence cautioni

The sequence AAO44561 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA Translation: AAO44561.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiAAO44561; AAO44561; TWT_464
KEGGitwh:TWT_464

Similar proteinsi

Entry informationi

Entry nameiLIPA_TROWT
AccessioniPrimary (citable) accession number: Q83MU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: April 25, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health