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Q83MU9 (LIPA_TROWT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase

EC=2.8.1.8
Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene names
Name:lipA
Ordered Locus Names:TWT_464
OrganismTropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]
Taxonomic identifier203267 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Sequence caution

The sequence AAO44561.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Lipoyl synthase HAMAP-Rule MF_00206
PRO_0000102376

Sites

Metal binding741Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding791Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding851Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1001Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1041Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1071Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83MU9 [UniParc].

Last modified May 10, 2004. Version 2.
Checksum: 998C3989CADF3E18

FASTA35139,596
        10         20         30         40         50         60 
MNDSGNSSKV NVRPPSAGLG APSPGKRKML RLEVRNSQVP IERKPSWIRA RATIGTEYRK 

        70         80         90        100        110        120 
VQERVKKQNL RTVCQEAGCP NIYECWEDRE ATFLIGGSQC TRRCDFCQID TGKPAELDLD 

       130        140        150        160        170        180 
EPKRVGQSVA QMKLRYATVT GVARDDLPDG GVWLYAETIR EIHKQCPGSG VEILIPDFNG 

       190        200        210        220        230        240 
KPELLQQIFE AQPEVYAHNI ETVPRIFRRI RPAFRYDRSL DVISQGQKAG MITKSNLILG 

       250        260        270        280        290        300 
MGETGEEVTQ ALRDLKSAGC DIVTITQYLR PSPRHLPVAR WVKPQEFIEY KEQAKEIGFS 

       310        320        330        340        350 
GVLAGPLVRS SYRAGKLWAQ SVKAKMVEIP ERVRKLINEI EMQETSFRQA V 

« Hide

References

[1]"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Twist.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014184 Genomic DNA. Translation: AAO44561.1. Different initiation.
RefSeqNP_787592.2. NC_004572.3.

3D structure databases

ProteinModelPortalQ83MU9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203267.TWT464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO44561; AAO44561; TWT_464.
GeneID1089343.
KEGGtwh:TWT464.
PATRIC23997471. VBITroWhi72342_0606.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0320.
KOK03644.
OMAEEYVTPE.
OrthoDBEOG6038ZS.
ProtClustDBPRK05481.

Enzyme and pathway databases

BioCycTWHI203267:GJDK-495-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_TROWT
AccessionPrimary (citable) accession number: Q83MU9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways