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Q83MU9

- LIPA_TROWT

UniProt

Q83MU9 - LIPA_TROWT

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Protein

Lipoyl synthase

Gene

lipA

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-495-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:TWT_464
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000002200: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Lipoyl synthasePRO_0000102376Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203267.TWT464.

Structurei

3D structure databases

ProteinModelPortaliQ83MU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiWIKIRAK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83MU9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNDSGNSSKV NVRPPSAGLG APSPGKRKML RLEVRNSQVP IERKPSWIRA
60 70 80 90 100
RATIGTEYRK VQERVKKQNL RTVCQEAGCP NIYECWEDRE ATFLIGGSQC
110 120 130 140 150
TRRCDFCQID TGKPAELDLD EPKRVGQSVA QMKLRYATVT GVARDDLPDG
160 170 180 190 200
GVWLYAETIR EIHKQCPGSG VEILIPDFNG KPELLQQIFE AQPEVYAHNI
210 220 230 240 250
ETVPRIFRRI RPAFRYDRSL DVISQGQKAG MITKSNLILG MGETGEEVTQ
260 270 280 290 300
ALRDLKSAGC DIVTITQYLR PSPRHLPVAR WVKPQEFIEY KEQAKEIGFS
310 320 330 340 350
GVLAGPLVRS SYRAGKLWAQ SVKAKMVEIP ERVRKLINEI EMQETSFRQA

V
Length:351
Mass (Da):39,596
Last modified:May 10, 2004 - v2
Checksum:i998C3989CADF3E18
GO

Sequence cautioni

The sequence AAO44561.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44561.1. Different initiation.
RefSeqiNP_787592.2. NC_004572.3.

Genome annotation databases

EnsemblBacteriaiAAO44561; AAO44561; TWT_464.
GeneIDi1089343.
KEGGitwh:TWT464.
PATRICi23997471. VBITroWhi72342_0606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44561.1 . Different initiation.
RefSeqi NP_787592.2. NC_004572.3.

3D structure databases

ProteinModelPortali Q83MU9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203267.TWT464.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO44561 ; AAO44561 ; TWT_464 .
GeneIDi 1089343.
KEGGi twh:TWT464.
PATRICi 23997471. VBITroWhi72342_0606.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OMAi WIKIRAK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci TWHI203267:GJDK-495-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
    Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
    Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Twist.

Entry informationi

Entry nameiLIPA_TROWT
AccessioniPrimary (citable) accession number: Q83MU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 26, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3