fumC

Functionⁱ

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityⁱ

(S)-malate = fumarate + H₂O.UniRule annotation

Pathwayⁱ: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:

Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)

This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	126	SubstrateUniRule annotation	1
Binding siteⁱ	187	SubstrateUniRule annotation	1
Active siteⁱ	188	Proton donor/acceptorUniRule annotation	1
Active siteⁱ	318	UniRule annotation	1
Binding siteⁱ	319	SubstrateUniRule annotation	1
Siteⁱ	331	Important for catalytic activityUniRule annotation	1

GO - Molecular functionⁱ

fumarate hydratase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fumarate metabolic process Source: InterPro
tricarboxylic acid cycle Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayⁱ	UPA00223; UER01007

UniPathwayⁱ

UPA00223; UER01007

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation) Short name: Fumarase CUniRule annotation Alternative name(s): Aerobic fumaraseUniRule annotation Iron-independent fumaraseUniRule annotation
Gene namesⁱ	Name:fumCUniRule annotation Ordered Locus Names:SF1634, S1765
Organismⁱ	Shigella flexneri
Taxonomic identifierⁱ	623 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Shigella
Proteomesⁱ	UP000002673 Componentⁱ: Chromosome UP000001006 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00743

GO - Cellular componentⁱ

tricarboxylic acid cycle enzyme complex Source: InterPro

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000161310	1 – 467	Fumarate hydratase class IIAdd BLAST		467

Interactionⁱ

Subunit structureⁱ

Homotetramer.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q83ML8
SMRⁱ	Q83ML8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	98 – 100	Substrate bindingUniRule annotation	3
Regionⁱ	129 – 132	Substrate binding (B site)UniRule annotation	4
Regionⁱ	139 – 141	Substrate bindingUniRule annotation	3
Regionⁱ	324 – 326	Substrate bindingUniRule annotation	3

Sequence similaritiesⁱ

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C9Q Bacteria COG0114 LUCA
HOGENOMⁱ	HOG000061736
KEGG Orthology (KO) More...KOⁱ	K01679
OMAⁱ	VSFTDNC

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01362 Fumarase_classII, 1 hit
Gene3Dⁱ	1.10.275.101 hit
HAMAPⁱ	MF_00743 FumaraseC, 1 hit
InterProⁱ	View protein in InterPro IPR005677 Fum_hydII IPR024083 Fumarase/histidase_N IPR018951 Fumarase_C_C IPR020557 Fumarate_lyase_CS IPR000362 Fumarate_lyase_fam IPR022761 Fumarate_lyase_N IPR008948 L-Aspartase-like
PANTHERⁱ	PTHR11444 PTHR11444, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10415 FumaraseC_C, 1 hit PF00206 Lyase_1, 1 hit
PRINTSⁱ	PR00149 FUMRATELYASE
SUPFAMⁱ	SSF48557 SSF48557, 1 hit
TIGRFAMsⁱ	TIGR00979 fumC_II, 1 hit
PROSITEⁱ	View protein in PROSITE PS00163 FUMARATE_LYASES, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q83ML8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNTVRSEKDS MGAIDVPADK LWGAQTQRSL EHFRISTEKM PTSLIHALAL 
        60         70         80         90        100
TKRAAAKVNE DLDLLSEEKA SAIRQAADEV LAGQHDDEFP LAIWQTGSGT 
       110        120        130        140        150
QSNMNMNEVL ANRASELLGG VRGMERKVHP NDDVNKSQSS NDVFPTAMHV 
       160        170        180        190        200
AALLALRKQL IPQLKTLTQT LSEKSRAFAD IVKIGRTHLQ DATPLTLGQE 
       210        220        230        240        250
ISGWVAMLEH NLKHIEYSLP HVAELALGGT AVGTGLNTHP EYARRVADEL 
       260        270        280        290        300
AVITCAPFVT APNKFEALAT CDALVQAHGA LKGLAASLMK IANDVRWLAS 
       310        320        330        340        350
GPRCGIGEIS IPENEPGSSI MPGKVNPTQC EALTMLCCQV MGNDVAINMG 
       360        370        380        390        400
GASGNFELNV FRPMVIHNFL QSVRLLADGM ESFNKHCAVG IEPNRERINQ 
       410        420        430        440        450
LLNESLMLVT ALNTHIGYDK AAEIAKKAHK EGLTLKAAAL ALGYLSEAEF 
       460 
DSWVRPEQMV GSMKAGR

Length:467

Mass (Da):50,520

Last modified:June 1, 2003 - v1

Checksum:ⁱ865CBAE31CB2768D

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE005674 Genomic DNA Translation: AAN43217.1 AE014073 Genomic DNA Translation: AAP17104.1
NCBI Reference Sequences More...RefSeqⁱ	NP_707510.1, NC_004337.2 WP_001099068.1, NZ_MTPK01000030.1

Genome annotation databases

EnsemblBacteriaⁱ	AAN43217; AAN43217; SF1634 AAP17104; AAP17104; S1765
GeneIDⁱ	1027791
KEGGⁱ	sfl:SF1634 sfx:S1765
PATRICⁱ	fig\|198214.7.peg.1927

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q83ML8	I6H3Z9 A0A127GK46 A0A1W2MU10 Q0T4G5 A0A200LGV9 D2AGB0 A0A226K915 I6BTI9	Shigella flexneri 1235-66 Shigella flexneri 4c Shigella flexneri Shigella flexneri serotype 5b (strain 8401) Shigella sonnei Shigella flexneri serotype X (strain 2002017) Shigella flexneri 2a str. 301 Shigella flexneri 2850-71	467	UniRef100_Q83ML8	Cluster: Fumarate hydratase class II	9

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q83ML8	P05042 W1B7T2 A0A024L3D9 A0A125X3E5 W1X103 H4UJA0 A0A070SU67 M9GZM5 A0A073HGC8 A0A236HKT4 +1092	Escherichia coli (strain K12) Klebsiella pneumoniae IS22 Escherichia coli Escherichia coli 1-110-08_S3_C1 Escherichia coli DORA_A_5_14_21 Escherichia coli DEC6A Escherichia coli 2-210-07_S3_C3 Escherichia coli MP021561.2 Escherichia coli 5-366-08_S1_C3 Shigella sonnei And more	467	UniRef90_P05042	Cluster: Fumarate hydratase class II	1,103

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q83ML8	Q92PB6 F7X7C7 H0G8G5 A0A222H881 UPI000292D36A A0A0E0UDK6 UPI000944B1FA A0A249NTI2 UPI000A312863 A0A1L3L3R4 +10423	Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) Sinorhizobium meliloti (strain SM11) Sinorhizobium meliloti CCNWSX0020 Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) Sinorhizobium meliloti (strain BL225C) Sinorhizobium sp. CCBAU 05631 Sinorhizobium americanum CCGM7 Sinorhizobium americanum Sinorhizobium medicae Sinorhizobium medicae (strain WSM419) (Ensifer medicae) And more	463	UniRef50_Q92PB6	Cluster: Fumarate hydratase class II	10,434

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE005674 Genomic DNA Translation: AAN43217.1 AE014073 Genomic DNA Translation: AAP17104.1
NCBI Reference Sequences More...RefSeqⁱ	NP_707510.1, NC_004337.2 WP_001099068.1, NZ_MTPK01000030.1

3D structure databases

ProteinModelPortalⁱ	Q83ML8
SMRⁱ	Q83ML8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAN43217; AAN43217; SF1634 AAP17104; AAP17104; S1765
GeneIDⁱ	1027791
KEGGⁱ	sfl:SF1634 sfx:S1765
PATRICⁱ	fig\|198214.7.peg.1927

Phylogenomic databases

eggNOGⁱ	ENOG4105C9Q Bacteria COG0114 LUCA
HOGENOMⁱ	HOG000061736
KEGG Orthology (KO) More...KOⁱ	K01679
OMAⁱ	VSFTDNC

Enzyme and pathway databases

UniPathwayⁱ	UPA00223; UER01007

UniPathwayⁱ

UPA00223; UER01007

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01362 Fumarase_classII, 1 hit
Gene3Dⁱ	1.10.275.101 hit
HAMAPⁱ	MF_00743 FumaraseC, 1 hit
InterProⁱ	View protein in InterPro IPR005677 Fum_hydII IPR024083 Fumarase/histidase_N IPR018951 Fumarase_C_C IPR020557 Fumarate_lyase_CS IPR000362 Fumarate_lyase_fam IPR022761 Fumarate_lyase_N IPR008948 L-Aspartase-like
PANTHERⁱ	PTHR11444 PTHR11444, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10415 FumaraseC_C, 1 hit PF00206 Lyase_1, 1 hit
PRINTSⁱ	PR00149 FUMRATELYASE
SUPFAMⁱ	SSF48557 SSF48557, 1 hit
TIGRFAMsⁱ	TIGR00979 fumC_II, 1 hit
PROSITEⁱ	View protein in PROSITE PS00163 FUMARATE_LYASES, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FUMC_SHIFL
Accessionⁱ	Q83ML8Primary (citable) accession number: Q83ML8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
	Last sequence update:	June 1, 2003
	Last modified:	April 25, 2018
	This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q83ML8 (FUMC_SHIFL)

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Fumarate hydratase class II

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tricarboxylic acid cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: tricarboxylic acid cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ