UniProtKB - Q83ML8 (FUMC_SHIFL)
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Protein
Fumarate hydratase class II
Gene
fumC
Organism
Shigella flexneri
Status
Functioni
Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation
Miscellaneous
There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation
Catalytic activityi
(S)-malate = fumarate + H2O.UniRule annotation
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 126 | SubstrateUniRule annotation | 1 | |
Binding sitei | 187 | SubstrateUniRule annotation | 1 | |
Active sitei | 188 | Proton donor/acceptorUniRule annotation | 1 | |
Active sitei | 318 | UniRule annotation | 1 | |
Binding sitei | 319 | SubstrateUniRule annotation | 1 | |
Sitei | 331 | Important for catalytic activityUniRule annotation | 1 |
GO - Molecular functioni
- fumarate hydratase activity Source: UniProtKB-EC
GO - Biological processi
- fumarate metabolic process Source: InterPro
- tricarboxylic acid cycle Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Biological process | Tricarboxylic acid cycle |
Enzyme and pathway databases
UniPathwayi | UPA00223; UER01007 |
Names & Taxonomyi
Protein namesi | Recommended name: Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)Short name: Fumarase CUniRule annotation Alternative name(s): Aerobic fumaraseUniRule annotation Iron-independent fumaraseUniRule annotation |
Gene namesi | Name:fumCUniRule annotation Ordered Locus Names:SF1634, S1765 |
Organismi | Shigella flexneri |
Taxonomic identifieri | 623 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Shigella |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- tricarboxylic acid cycle enzyme complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161310 | 1 – 467 | Fumarate hydratase class IIAdd BLAST | 467 |
Interactioni
Subunit structurei
Homotetramer.UniRule annotation
Structurei
3D structure databases
ProteinModelPortali | Q83ML8 |
SMRi | Q83ML8 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 98 – 100 | Substrate bindingUniRule annotation | 3 | |
Regioni | 129 – 132 | Substrate binding (B site)UniRule annotation | 4 | |
Regioni | 139 – 141 | Substrate bindingUniRule annotation | 3 | |
Regioni | 324 – 326 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105C9Q Bacteria COG0114 LUCA |
HOGENOMi | HOG000061736 |
KOi | K01679 |
OMAi | VSFTDNC |
Family and domain databases
CDDi | cd01362 Fumarase_classII, 1 hit |
Gene3Di | 1.10.275.101 hit |
HAMAPi | MF_00743 FumaraseC, 1 hit |
InterProi | View protein in InterPro IPR005677 Fum_hydII IPR024083 Fumarase/histidase_N IPR018951 Fumarase_C_C IPR020557 Fumarate_lyase_CS IPR000362 Fumarate_lyase_fam IPR022761 Fumarate_lyase_N IPR008948 L-Aspartase-like |
PANTHERi | PTHR11444 PTHR11444, 1 hit |
Pfami | View protein in Pfam PF10415 FumaraseC_C, 1 hit PF00206 Lyase_1, 1 hit |
PRINTSi | PR00149 FUMRATELYASE |
SUPFAMi | SSF48557 SSF48557, 1 hit |
TIGRFAMsi | TIGR00979 fumC_II, 1 hit |
PROSITEi | View protein in PROSITE PS00163 FUMARATE_LYASES, 1 hit |
i Sequence
Sequence statusi: Complete.
Q83ML8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTVRSEKDS MGAIDVPADK LWGAQTQRSL EHFRISTEKM PTSLIHALAL
60 70 80 90 100
TKRAAAKVNE DLDLLSEEKA SAIRQAADEV LAGQHDDEFP LAIWQTGSGT
110 120 130 140 150
QSNMNMNEVL ANRASELLGG VRGMERKVHP NDDVNKSQSS NDVFPTAMHV
160 170 180 190 200
AALLALRKQL IPQLKTLTQT LSEKSRAFAD IVKIGRTHLQ DATPLTLGQE
210 220 230 240 250
ISGWVAMLEH NLKHIEYSLP HVAELALGGT AVGTGLNTHP EYARRVADEL
260 270 280 290 300
AVITCAPFVT APNKFEALAT CDALVQAHGA LKGLAASLMK IANDVRWLAS
310 320 330 340 350
GPRCGIGEIS IPENEPGSSI MPGKVNPTQC EALTMLCCQV MGNDVAINMG
360 370 380 390 400
GASGNFELNV FRPMVIHNFL QSVRLLADGM ESFNKHCAVG IEPNRERINQ
410 420 430 440 450
LLNESLMLVT ALNTHIGYDK AAEIAKKAHK EGLTLKAAAL ALGYLSEAEF
460
DSWVRPEQMV GSMKAGR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005674 Genomic DNA Translation: AAN43217.1 AE014073 Genomic DNA Translation: AAP17104.1 |
RefSeqi | NP_707510.1, NC_004337.2 WP_001099068.1, NZ_MTPK01000030.1 |
Genome annotation databases
EnsemblBacteriai | AAN43217; AAN43217; SF1634 AAP17104; AAP17104; S1765 |
GeneIDi | 1027791 |
KEGGi | sfl:SF1634 sfx:S1765 |
PATRICi | fig|198214.7.peg.1927 |
Similar proteinsi
Entry informationi
Entry namei | FUMC_SHIFL | |
Accessioni | Q83ML8Primary (citable) accession number: Q83ML8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 25, 2018 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |