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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. 2-dehydropantoate 2-reductase (panE), 2-dehydropantoate 2-reductase (CEG98_17360), 2-dehydropantoate 2-reductase (RK59_019385), 2-dehydropantoate 2-reductase (CSW75_08200)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45MagnesiumUniRule annotation1
Metal bindingi84MagnesiumUniRule annotation1
Binding sitei84Alpha-ketoisovalerateUniRule annotation1
Binding sitei112Alpha-ketoisovalerateUniRule annotation1
Metal bindingi114MagnesiumUniRule annotation1
Active sitei181Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:SF0131, S0133
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001848871 – 2643-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST264

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ83ME5
SMRiQ83ME5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 46Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiGHIGLMP

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Q83ME5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPTTIASLQ KYKQDKKRFA TITAYDYSFA KLFADEGLNV MLVGDSLGMT
60 70 80 90 100
VQGHDSTLPV TVADIAYHTA AVRRGAPNCL LLADLPFMAY ATPEQAFENA
110 120 130 140 150
ATVMRAGANM VKIEGGEWLV ETVKMLTERA VPVCGHLGLT PQSVNIFGGY
160 170 180 190 200
KVQGRGNEAS DRLLSDALAL EAAGAQLLVL ECVPVELAKR ITEALAIPVI
210 220 230 240 250
GIGAGNVTDG QILVMHDAFG ITGGHIPKFA KNFLAETGDI RAAVRQYMAE
260
VESGVYPGEE HSFH
Length:264
Mass (Da):28,238
Last modified:June 1, 2003 - v1
Checksum:i6AA932846D0B9CD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN41794.1
AE014073 Genomic DNA Translation: AAP15675.1
RefSeqiNP_706087.1, NC_004337.2
WP_000805476.1, NZ_MTPK01000013.1

Genome annotation databases

EnsemblBacteriaiAAN41794; AAN41794; SF0131
AAP15675; AAP15675; S0133
GeneIDi1024497
KEGGisfl:SF0131
sfx:S0133
PATRICifig|198214.7.peg.147

Similar proteinsi

Entry informationi

Entry nameiPANB_SHIFL
AccessioniPrimary (citable) accession number: Q83ME5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health