Reviewed, UniProtKB/Swiss-Prot Q83M39 (AES_SHIFL)

Names and origin

Protein names

Recommended name:
    Acetyl esterase
    EC=3.1.1.-

Gene names

Name:	aes
Ordered Locus Names:	SF0421, S0428

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively regulates malT activity by antagonizing maltotriose binding. Inhibits melA galactosidase activity By similarity.
Subunit structure	Homodimer. Interacts with malT and melA By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the 'GDXG' lipolytic enzyme family.
Sequence caution	The sequence AAN42076.1 differs from that shown. Reason: Erroneous termination at position 273. Translated as Gln. The sequence AAP15953.1 differs from that shown. Reason: Erroneous termination at position 273. Translated as Gln.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 319	319	Acetyl esterase
Sites
	Active site	165	1	By similarity
	Active site	262	1	By similarity
	Active site	292	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83M39-1 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: A61E91C47CEB35D6	FASTA	319	36,037
	10 20 30 40 50 60 MKPENKLPVL DLISAEMKTV VNTLQPDLPS WPATGTIAEQ RQYYTLERRF WNAGAPEMAT 70 80 90 100 110 120 RAYMVPTKYG QVETRLFCPQ PDSPATLFYL HGGGFILGNL DTHDRIMRLL ASYSQCTVIG 130 140 150 160 170 180 INYTLSPEAR FPQAIEEIVA ACCYFHQQAE DYQINMSRIG FAGDSAGAML ALASALWLRD 190 200 210 220 230 240 KQIDCGKIAG VLLWYGLYGL RDSVTRRLLG GVWDGLTQQD LQMYEEAYLS NDADRESPYY 250 260 270 280 290 300 CLFNNDLTRE VPPCFIAGAE FDPLLDDSRL LYQTLAAHQQ PCEFKLYPGT LHAFLHYSRM 310 MKTADEALRD GAQFFTAQL

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN42076.1. Sequence problems. AE014073 Genomic DNA. Translation: AAP15953.1. Sequence problems.
RefSeq	NP_706369.1. NP_836147.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1027722. 1076865.
GenomeReviews	Gene locus S0428 in contig AE014073_GR. Gene locus SF0421 in contig AE005674_GR.
KEGG	sfl:SF0421. sfx:S0428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q83M39.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN42076.1-MON.
Family and domain databases
HAMAP	MF_01958. [Tree]
InterPro	IPR013094. AB_hydrolase_3. IPR002168. Lipase_GDXG_AS. [Graphical view]
Pfam	PF07859. Abhydrolase_3. 1 hit. [Graphical view]
PROSITE	PS01173. LIPASE_GDXG_HIS. 1 hit. PS01174. LIPASE_GDXG_SER. 1 hit. [Graphical view]
ProDom	Q83M39. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AES_SHIFL

Accession

Primary (citable) accession number: Q83M39
Secondary accession number(s): Q7C2W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	July 22, 2008
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)