Reviewed, UniProtKB/Swiss-Prot Q83L52 (ASTA_SHIFL)

Names and origin

Protein names

Recommended name:
    Arginine N-succinyltransferase
      Short name=AST
    EC=2.3.1.109
Alternative name(s):
    AOST

Gene names

Name:	astA
Ordered Locus Names:	SF1479, S1596

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine By similarity.
Catalytic activity	Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine. HAMAP MF_01171
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 1/5. HAMAP MF_01171
Sequence similarities	Belongs to the arginine N-succinyltransferase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	arginine N-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Arginine N-succinyltransferase HAMAP MF_01171		PRO_0000262332
Sites
	Active site	229	1	Proton donor Potential
	Binding site	125	1	Succinyl-CoA; via amide nitrogen Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83L52-1 [UniParc]. Last modified November 28, 2006. Version 5. Checksum: 0B4D80412A880BF1	FASTA	344	38,428
	10 20 30 40 50 60 MMVIRPVERS DVSALMQLAS KTGGGLTSLP ANEATLSARI ERAIKTWQGE LPKSEQGYVF 70 80 90 100 110 120 VLEDSETGTV AGICAIEVAV GLNDPWYNYR VGTLVHASKE LNVYNALPTL FLSNDHTGSS 130 140 150 160 170 180 ELCTLFLDPD WRKEGNGYLL SKSRFMFMAA FRDKFNDKVV AEMRGVIDEH GYSPFWQSLG 190 200 210 220 230 240 KRFFSMDFSR ADFLCGTGQK AFIAELMPKH PIYTHFLSQE AQDVIGQVHP QTAPARAVLE 250 260 270 280 290 300 KEGFRYRNYI DIFDGGPTLE CDIDRVRAIR KSRLVEVAEG QPAQGDFPAC LVANENYHHF 310 320 330 340 RVVLARTDPA TERLILTAAQ LDALKCHAGD RVRLVRLCAE EKTA

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN43072.2. Different initiation. AE014073 Genomic DNA. Translation: AAP16965.1. Different initiation.
RefSeq	NP_707365.2. NP_837158.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1024657. 1077968.
GenomeReviews	Gene locus SF1479 in contig AE005674_GR. Gene locus S1596 in contig AE014073_GR.
KEGG	sfl:SF1479. sfx:S1596.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q83L52.
OMA	Q83L52. TLFLAND.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN43072.1-MON.
BRENDA	2.3.1.109. 189495.
Family and domain databases
HAMAP	MF_01171. [Tree]
InterPro	IPR007041. Arg_N-succinylTrfase_Ast/AOST. IPR017650. Arginine_N-succinylTrfase_AstA. [Graphical view]
Pfam	PF04958. AstA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03243. arg_catab_AOST. 1 hit. TIGR03244. arg_catab_AstA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTA_SHIFL

Accession

Primary (citable) accession number: Q83L52
Secondary accession number(s): Q7UCI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	June 16, 2009
This is version 43 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)