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Q83L36

- SYFB_SHIFL

UniProt

Q83L36 - SYFB_SHIFL

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi454 – 4541MagnesiumUniRule annotation
    Metal bindingi460 – 4601Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi463 – 4631MagnesiumUniRule annotation
    Metal bindingi464 – 4641MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Short name:
    PheRSUniRule annotation
    Gene namesi
    Name:pheTUniRule annotation
    Ordered Locus Names:SF1518, S1635
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 795795Phenylalanine--tRNA ligase beta subunitPRO_0000126946Add
    BLAST

    Proteomic databases

    PaxDbiQ83L36.
    PRIDEiQ83L36.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi198214.SF1518.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83L36.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 148110tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini401 – 47676B5UniRule annotationAdd
    BLAST
    Domaini701 – 79494FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0073.
    HOGENOMiHOG000292085.
    KOiK01890.
    OMAiNYVMIEL.
    OrthoDBiEOG6CCH1J.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83L36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFSELWLRE WVNPAIDSDT LANQITMAGL EVDGVEPVAG SFHGVVVGEV    50
    VECAQHPNAD KLRVTKVNVG GDRLLDIVCG APNCRQGLRV AVATIGAVLP 100
    GDFKIKAAKL RGEPSEGMLC SFSELGISDD HSGIIELPAD APIGTDIREY 150
    LKLDDNTIEI SVTPNRADCL GIIGVARDVA VLNQLPLVEP EIVPVGATID 200
    DTLPITVEAP EACPRYLGRV VKGINVKAPT PLWMKEKLRR CGIRSIDAVV 250
    DVTNYVLLEL GQPMHAFDKD RIEGGIVVRM AKEGETLVLL DGTEAKLNAD 300
    TLVIADHNKA LAMGGIFGGE HSGVNDETQN VLLECAFFSP LSITGRARRH 350
    GLHTDASHRY ERGVDPVLQH KAMERATRLL IDICGGEAGP VIDITNEATL 400
    PKRATITLRR SKLDRLIGHH IADEQVTDIL RRLGCEVTEG KDEWQAVAPS 450
    WRFDMEIEED LVEEVARVYG YNNIPDEPVQ ASLIMGTHRE ADLSLKRVKT 500
    LLNDKGYQEV ITYSFVDPKV QQMIHPGVEA LLLASPISVE MSAMRLSLWT 550
    GLLATVVYNQ NRQQNRVRIF ESGLRFVPDT QAPLGIRQDL MLAGVICGNR 600
    YEEHWNLAKE TVDFYDLKGD LESVLDLTGK LNEVEFRAEA NPALHPGQSA 650
    AIYLKGERIG FVGVVHPELE RKLDLNGRTL VFELEWNKLA DRVVPQAREI 700
    SRFPANRRDI AVVVAENVPA ADILSECKKV GVNQVVGVNL FDVYRGKGVA 750
    EGYKSLAISL ILQDTSRTLE EEEIAATVAK CVEALKERFQ ASLRD 795
    Length:795
    Mass (Da):87,411
    Last modified:June 1, 2003 - v1
    Checksum:i7F59E6BE805AB0A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN43108.1.
    AE014073 Genomic DNA. Translation: AAP16998.1.
    RefSeqiNP_707401.1. NC_004337.2.
    NP_837191.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN43108; AAN43108; SF1518.
    AAP16998; AAP16998; S1635.
    GeneIDi1024717.
    1079642.
    KEGGisfl:SF1518.
    sfx:S1635.
    PATRICi18704628. VBIShiFle31049_1816.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN43108.1 .
    AE014073 Genomic DNA. Translation: AAP16998.1 .
    RefSeqi NP_707401.1. NC_004337.2.
    NP_837191.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali Q83L36.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF1518.

    Proteomic databases

    PaxDbi Q83L36.
    PRIDEi Q83L36.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN43108 ; AAN43108 ; SF1518 .
    AAP16998 ; AAP16998 ; S1635 .
    GeneIDi 1024717.
    1079642.
    KEGGi sfl:SF1518.
    sfx:S1635.
    PATRICi 18704628. VBIShiFle31049_1816.

    Phylogenomic databases

    eggNOGi COG0073.
    HOGENOMi HOG000292085.
    KOi K01890.
    OMAi NYVMIEL.
    OrthoDBi EOG6CCH1J.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiSYFB_SHIFL
    AccessioniPrimary (citable) accession number: Q83L36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3