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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM), Hydroxyethylthiazole kinase (thiM), Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Substrate; via amide nitrogenUniRule annotation1
Binding sitei125ATPUniRule annotation1
Binding sitei171ATPUniRule annotation1
Binding sitei198Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:SF2166, S2291
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569511 – 262Hydroxyethylthiazole kinaseAdd BLAST262

Structurei

3D structure databases

ProteinModelPortaliQ83KH2
SMRiQ83KH2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

Q83KH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTRWTLDP
110 120 130 140 150
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA
160 170 180 190 200
ANAIPAAQTL ARETGAIVVV TGEVDYVTDG HRIIGIHGGD PLMTKVVGTG
210 220 230 240 250
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL
260
DALWQLTQEV QA
Length:262
Mass (Da):27,396
Last modified:June 1, 2003 - v1
Checksum:iA5B23ED41EBFA5C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN43697.1
AE014073 Genomic DNA Translation: AAP17523.1
RefSeqiNP_707990.1, NC_004337.2
WP_001195611.1, NZ_MTPK01000079.1

Genome annotation databases

EnsemblBacteriaiAAN43697; AAN43697; SF2166
AAP17523; AAP17523; S2291
GeneIDi1023547
KEGGisfl:SF2166
sfx:S2291
PATRICifig|198214.7.peg.2583

Similar proteinsi

Entry informationi

Entry nameiTHIM_SHIFL
AccessioniPrimary (citable) accession number: Q83KH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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