Reviewed, UniProtKB/Swiss-Prot Q83K86 (GLK_SHIFL)

Names and origin

Protein names

Recommended name:
    Glucokinase
    EC=2.7.1.2
Alternative name(s):
    Glucose kinase

Gene names

Name:	glk
Ordered Locus Names:	SF2454, S2593

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP MF_00524
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the bacterial glucokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Glucokinase HAMAP MF_00524		PRO_0000215139
Regions
	Nucleotide binding	8 – 13	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83K86-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2D6CC1E1341A3823	FASTA	321	34,772
	10 20 30 40 50 60 MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC 70 80 90 100 110 120 IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG 130 140 150 160 170 180 GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE AIILEILRAE 190 200 210 220 230 240 IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI 250 260 270 280 290 300 MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 310 320 HDYPGLLGSG AHLRQTLGHI L

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN43962.1. AE014073 Genomic DNA. Translation: AAP17773.1.
RefSeq	NP_708255.1. NP_837963.1.
3D structure databases
SMR	Q83K86. Positions 2-321.
ModBase	Search...
Genome annotation databases
GeneID	1025571. 1078864.
GenomeReviews	Gene locus SF2454 in contig AE005674_GR. Gene locus S2593 in contig AE014073_GR.
KEGG	sfl:SF2454. sfx:S2593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q83K86.
OMA	Q83K86. VDIGGTH.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN43962.1-MON.
BRENDA	2.7.1.2. 189495.
Family and domain databases
HAMAP	MF_00524. [Tree]
InterPro	IPR003836. Glucokinase. [Graphical view]
Pfam	PF02685. Glucokinase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00749. glk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLK_SHIFL

Accession

Primary (citable) accession number: Q83K86
Secondary accession number(s): Q7C0L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)