Reviewed,
UniProtKB/Swiss-Prot Q83K39 (HCAE_SHIFL)
Last modified
November 25, 2008.
Version 45.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha EC=1.14.12.19 Alternative name(s): Digoxigenin subunit alpha | ||||
| Gene names |
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| Organism | Shigella flexneri [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 623 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella |
Protein attributes
| Sequence length | 453 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. |
| Catalytic activity | 3-phenylpropanoic acid + NADH + O(2) = cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD(+). Cinnamic acid + H(+) + NADH + O(2) = cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD(+). |
| Cofactor | Binds 1 iron ion By similarity. Binds 1 2Fe-2S cluster per subunit By similarity. |
| Pathway | Aromatic compound metabolism; 3-phenylpropionic acid degradation. |
| Subunit structure | This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity. |
| Sequence similarities | Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Aromatic hydrocarbons catabolism |
| Ligand | 2Fe-2S Iron Iron-sulfur Metal-binding NAD |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reductionInferred from electronic annotation. Source: InterPro |
| Molecular function | 2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro 3-phenylpropionate dioxygenase activityInferred from electronic annotation. Source: HAMAP electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygenInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 453 | 453 | 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha | PRO_0000333707 | |||||
Regions | |||||||||
| Domain | 44 – 142 | 99 | Rieske | ||||||
Sites | |||||||||
| Metal binding | 85 | 1 | Iron-sulfur (2Fe-2S) By similarity | ||||||
| Metal binding | 87 | 1 | Iron-sulfur (2Fe-2S); via pros nitrogen By similarity | ||||||
| Metal binding | 105 | 1 | Iron-sulfur (2Fe-2S) By similarity | ||||||
| Metal binding | 108 | 1 | Iron-sulfur (2Fe-2S); via pros nitrogen By similarity | ||||||
| Metal binding | 213 | 1 | Iron By similarity | ||||||
| Metal binding | 218 | 1 | Iron By similarity | ||||||
Sequences
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References
| [1] | "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. Yu J.Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a. |
| [2] | "Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a. |
Cross-references
Sequence databases | |
|---|---|
| AE005674 Genomic DNA. Translation: AAN44084.1. AE014073 Genomic DNA. Translation: AAP17909.1. | |
| RefSeq | NP_708377.1. NP_838099.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EG9 based on UniProtKB P23094. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1026946. 1078029. |
| GenomeReviews | Gene locus S2757 in contig AE014073_GR. Gene locus SF2585 in contig AE005674_GR. |
| KEGG | sfl:SF2585. sfx:S2757. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q83K39. |
Enzyme and pathway databases | |
| BioCyc | SFLE198214:AAN44084.1-MON. |
Family and domain databases | |
| HAMAP | MF_01648. [Tree] |
| InterPro | IPR005806. Rieske_reg. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. IPR015879. Ring_hydroxy_dOase_asu_C. IPR001663. Rng_hydr_dOase-A. [Graphical view] |
| Gene3D | G3DSA:2.102.10.10. Rieske_reg. 1 hit. |
| PANTHER | PTHR21266:SF2. Rng_hydr_dOase-A. 1 hit. |
| Pfam | PF00355. Rieske. 1 hit. PF00848. Ring_hydroxyl_A. 1 hit. [Graphical view] |
| PRINTS | PR00090. RNGDIOXGNASE. |
| PROSITE | PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HCAE_SHIFL | ||||||||
| Accession | Primary (citable) accession number: Q83K39 Secondary accession number(s): Q7C0G4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


