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Reviewed, UniProtKB/Swiss-Prot Q83JY2 (SURE_SHIFL)

Last modified June 16, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Multifunctional protein surE
Including the following 2 domains:
    1- Recommended name:
            5'/3'-nucleotidase
              EC=3.1.3.5
              EC=3.1.3.6
        Alternative name(s):
            Nucleoside monophosphate phosphohydrolase
    2- Recommended name:
            Exopolyphosphatase
              EC=3.6.1.11
Gene names
Name: surE
Ordered Locus Names: SF2767, S2960
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs By similarity.

Catalytic activity

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060

A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate. HAMAP MF_00060

Cofactor

Binds 1 divalent metal cation per subunit By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the surE nucleotidase family.

Caution

Tyr-8 is present instead of the conserved Asp which is expected to be a metal-binding site residue.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 253253Multifunctional protein surE HAMAP MF_00060
PRO_0000111842

Sites

Metal binding91Divalent metal cation By similarity
Metal binding391Divalent metal cation By similarity
Metal binding921Divalent metal cation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83JY2-1 [UniParc].

Last modified December 15, 2003. Version 2.
Checksum: 191A97BAEF07A082

FASTA25326,980
        10         20         30         40         50         60 
MRILLSNYDG VHAPGIQTLA KALREFADVQ VVAPDRNRSG ASNSLTLESS LRTFTFENGD 

        70         80         90        100        110        120 
IAVQMGTPTD CVYLGVNALM RPRPDIVVSG INAGPNLGDD VIYSGTVAAA MEGRHLGFPA 

       130        140        150        160        170        180 
LAVSLDGHKH YDTAAAVTCS ILRALCKEPL RTGRILNINV QDLPLDQIKG IRVTRCGTRH 

       190        200        210        220        230        240 
PADQVIPQQD PRGNTLYWIG PPGGKCDAGP GTDFAAVDEG YVSITPLHVD LTAHSAQDVV 

       250 
SDWLNSVGVG TQW 

« Hide

References

[1]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[2]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

AE005674 Genomic DNA. Translation: AAN44256.1. Different initiation.
AE014073 Genomic DNA. Translation: AAP18082.1.
RefSeqNP_708549.2.
NP_838272.1.

3D structure databases

HSSPHSSP built from PDB template 1J9L based on UniProtKB P96112.
ModBaseSearch...

Genome annotation databases

GeneID1025770.
1079225.
GenomeReviewsGene locus SF2767 in contig AE005674_GR.
Gene locus S2960 in contig AE014073_GR.
KEGGsfl:SF2767.
sfx:S2960.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ83JY2.

Enzyme and pathway databases

BioCycSFLE198214:AAN44256.1-MON.
BRENDA3.1.3.5. 189495.
3.1.3.6. 189495.
3.6.1.11. 189495.

Family and domain databases

HAMAPMF_00060.
[Tree]
InterProIPR002828. SurE-like_Pase/nucleotidase.
[Graphical view]
Gene3DG3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.
PfamPF01975. SurE. 1 hit.
[Graphical view]
ProDomPD005378. SurE. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00087. surE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSURE_SHIFL
AccessionPrimary (citable) accession number: Q83JY2
Secondary accession number(s): Q7UBT1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents