Q83IC8 (DXR_TROW8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	TW099

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163728
Regions
	Nucleotide binding	7 – 36	30	NADP By similarity
Sites
	Metal binding	125	1	Divalent metal cation By similarity
	Metal binding	127	1	Divalent metal cation By similarity
	Metal binding	194	1	Divalent metal cation By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	127	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83IC8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 06F9E8FDE3100DF9	FASTA	368	39,894
	10 20 30 40 50 60 MQRVIIVGST GSIGTQAIDF ILKNRDSFLV VGLAASTQTS LLREQAQVFG TKNTAQGASE 70 80 90 100 110 120 AAELIEATEA DVVLNAITGA AGLLSTYATL KTGKRLALAN KESLIMGGKY FLDMTSFKGQ 130 140 150 160 170 180 ITPVDSEHSA IAQAMKSGKF SEVNKLILTA SGGPFYDRES LEGITLKDAL DHPTWNMGPM 190 200 210 220 230 240 ISINSATMFN KGLEIIEAHL LFGIPYSQID VVIHPQSIVH SMVEYKDGSV IAQASVADMT 250 260 270 280 290 300 LPIGYALSWP NRALNAVRPL EWGARQRWDF LPPTENSMRS INLARRAGEA GGVYPAVLNA 310 320 330 340 350 360 SNECAVEAFM AGKISFARII HVTETVFNLY TKQHTNRESA KNPIEVILEE DARTRELAKT VIENMSAD

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW08/27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX251410 Genomic DNA. Translation: CAD66783.1.
RefSeq	NP_789046.1. NC_004551.1.
3D structure databases
ProteinModelPortal	Q83IC8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83IC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064329.
GenomeReviews	Gene locus TW099 in contig BX072543_GR.
KEGG	tws:TW099.
PATRIC	23998520. VBITroWhi42739_0107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0743.
HOGENOM	HBG430762.
OMA	IHSMVEY.
PhylomeDB	Q83IC8.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	TWHI218496:TW0091-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 2 hits. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_TROW8

Accession

Primary (citable) accession number: Q83IC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program