Q83I18 (CLPP2_TROW8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 2

Gene names

Name:	clpP2
Ordered Locus Names:	TW284

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444		PRO_0000179707
Sites
	Active site	103	1	By similarity
	Active site	128	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83I18 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5754570E8F31C42C	FASTA	207	22,956
	10 20 30 40 50 60 MYPNSRYILP SLDERTAYGY KQVDPYTKLF EDRIVFLGVQ IDDASADDVM AQLLVLEGQD 70 80 90 100 110 120 AERDIIMYIN SPGGSFTAMT AIYDTMQYIR PQIQTVCLGQ AASAAAVILS AGTPGKRLAL 130 140 150 160 170 180 PNARILIHQP VVASSGYGQA SDIEIQAREI MRMREWLEKT LAQHSNKSVK QVSKDIDRDK 190 200 ILSSEQALEY GLIDQILVSR KAGLGKK

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW08/27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX251410 Genomic DNA. Translation: CAD66958.1.
RefSeq	NP_789220.1. NC_004551.1.
3D structure databases
ProteinModelPortal	Q83I18.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83I18.
Protein family/group databases
MEROPS	S14.009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064573.
GenomeReviews	Gene locus TW284 in contig BX072543_GR.
KEGG	tws:TW284.
PATRIC	23999021. VBITroWhi42739_0347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HBG558421.
OMA	QDPYTKL.
ProtClustDB	PRK12553.
Enzyme and pathway databases
BioCyc	TWHI218496:TW0265-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP2_TROW8

Accession

Primary (citable) accession number: Q83I18

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program