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Q83I16

- SYI_TROW8

UniProt

Q83I16 - SYI_TROW8

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Protein
Isoleucine--tRNA ligase
Gene
ileS, TW287
Organism
Tropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:TW287
OrganismiTropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Taxonomic identifieri218496 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000001413: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10561056Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098570Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi218496.TW287.

Structurei

3D structure databases

ProteinModelPortaliQ83I16.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi63 – 7311"HIGH" regionUniRule annotation
Add
BLAST
Motifi632 – 6365"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83I16-1 [UniParc]FASTAAdd to Basket

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MCDQGEVSSQ NSSDYKEQRP TPRPNLPKIE ESVLAFWSSD KTFEASLEQR     50
QHGKRWVFYD GPPFANGLPH FGHLLTGYIK DAIPRYQTMR GQYVPRVFGW 100
DTHGLPAELE AMKRLGITEK SQIESMGIAS FNEAARKSVL TYVDQWEEYV 150
NRQARWVDFK NGYKTLDLDY MESVLWAFKT LYKKGVIYEG YKVLPYCWND 200
QTPLSNHELR MDDEVYKQRL DDSLTVTFPL IGQKAKTCGL DGVAALAWTT 250
TPWTLPSNMA LIVSPNVEYV VVSSARQNSN SDFLLCKSSL DSYAECLGYE 300
SGQDARASIR RTLLGKEIEG IHYKPLFDYY ADLHNAFTIL SDNYVDVTEG 350
TGIVHASPAH GEDDKRVCDA FGVPTVVSIN DAACFTDVIS NYAGMHIFDA 400
NAVIRSDLSR DGRILRHESY KHSYPHCWRC RSPLIYKAVT SWFFRITDSV 450
NRMLELNQQI NWVPKSVKNG QFAKWLSSAK DWSISRTRYW GTPIPVWKSD 500
NPEYPRIDCY GSLKELEDDF GIKLTDLHRP EIDRLTRPNP DDPTGASTMR 550
RVPDVLDVWF DAASMPFAQL HYPFENIERF EANKSADFIV EYAGQIRGWF 600
YLLHAMSTAL FDGVAFKNAI CHGIVLGDDG QKASKSLRNY PDVYDVFENE 650
GSDAVRWYLI SSSILRGGSL IVSRKKIQDA IRQYITPLWS SWYFFHIYSE 700
AARPGGYKAR FSVDSQDILD RYILSKTGLL VEDVTRFMDS FDMASAALQL 750
RDFVAVLTNW YIRRSRDRFW DGSDTGAFDT LYTVLETLCR LGAVFVPMVS 800
EHVYKCLTNS RSVHLSDWPD VATFPNETGL VETMDRVRKI CSTGLSLRKR 850
LGIKARQPLS SAHIRVAQVG SLAQYKDIIS GELNVKTVSI EEGSCTQRML 900
KILPRVAGPR LAGDVQTVIA AARRGDWTDH DGHVTAGGIP LLENEYQLVA 950
GAQDSKNSQP LPFGGSVTLD TRIDETLRSE GVARDTVRQI QIARKEKDLN 1000
ITDRISLEVC VPDEQVKNNL LAFSELICKE TLCDRLDILV KKGIDGITVS 1050
LEKFRQ 1056
Length:1,056
Mass (Da):119,751
Last modified:June 1, 2003 - v1
Checksum:iD1D531A27E790967
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX251411 Genomic DNA. Translation: CAD66961.1.
RefSeqiNP_789223.1. NC_004551.1.

Genome annotation databases

EnsemblBacteriaiCAD66961; CAD66961; TW287.
GeneIDi1064576.
KEGGitws:TW287.
PATRICi23999027. VBITroWhi42739_0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX251411 Genomic DNA. Translation: CAD66961.1 .
RefSeqi NP_789223.1. NC_004551.1.

3D structure databases

ProteinModelPortali Q83I16.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218496.TW287.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD66961 ; CAD66961 ; TW287 .
GeneIDi 1064576.
KEGGi tws:TW287.
PATRICi 23999027. VBITroWhi42739_0350.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW08/27.

Entry informationi

Entry nameiSYI_TROW8
AccessioniPrimary (citable) accession number: Q83I16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi