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Q83I16

- SYI_TROW8

UniProt

Q83I16 - SYI_TROW8

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Tropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TW287
OrganismiTropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Taxonomic identifieri218496 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000001413: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10561056Isoleucine--tRNA ligasePRO_0000098570Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi218496.TW287.

Structurei

3D structure databases

ProteinModelPortaliQ83I16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi63 – 7311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83I16-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCDQGEVSSQ NSSDYKEQRP TPRPNLPKIE ESVLAFWSSD KTFEASLEQR
60 70 80 90 100
QHGKRWVFYD GPPFANGLPH FGHLLTGYIK DAIPRYQTMR GQYVPRVFGW
110 120 130 140 150
DTHGLPAELE AMKRLGITEK SQIESMGIAS FNEAARKSVL TYVDQWEEYV
160 170 180 190 200
NRQARWVDFK NGYKTLDLDY MESVLWAFKT LYKKGVIYEG YKVLPYCWND
210 220 230 240 250
QTPLSNHELR MDDEVYKQRL DDSLTVTFPL IGQKAKTCGL DGVAALAWTT
260 270 280 290 300
TPWTLPSNMA LIVSPNVEYV VVSSARQNSN SDFLLCKSSL DSYAECLGYE
310 320 330 340 350
SGQDARASIR RTLLGKEIEG IHYKPLFDYY ADLHNAFTIL SDNYVDVTEG
360 370 380 390 400
TGIVHASPAH GEDDKRVCDA FGVPTVVSIN DAACFTDVIS NYAGMHIFDA
410 420 430 440 450
NAVIRSDLSR DGRILRHESY KHSYPHCWRC RSPLIYKAVT SWFFRITDSV
460 470 480 490 500
NRMLELNQQI NWVPKSVKNG QFAKWLSSAK DWSISRTRYW GTPIPVWKSD
510 520 530 540 550
NPEYPRIDCY GSLKELEDDF GIKLTDLHRP EIDRLTRPNP DDPTGASTMR
560 570 580 590 600
RVPDVLDVWF DAASMPFAQL HYPFENIERF EANKSADFIV EYAGQIRGWF
610 620 630 640 650
YLLHAMSTAL FDGVAFKNAI CHGIVLGDDG QKASKSLRNY PDVYDVFENE
660 670 680 690 700
GSDAVRWYLI SSSILRGGSL IVSRKKIQDA IRQYITPLWS SWYFFHIYSE
710 720 730 740 750
AARPGGYKAR FSVDSQDILD RYILSKTGLL VEDVTRFMDS FDMASAALQL
760 770 780 790 800
RDFVAVLTNW YIRRSRDRFW DGSDTGAFDT LYTVLETLCR LGAVFVPMVS
810 820 830 840 850
EHVYKCLTNS RSVHLSDWPD VATFPNETGL VETMDRVRKI CSTGLSLRKR
860 870 880 890 900
LGIKARQPLS SAHIRVAQVG SLAQYKDIIS GELNVKTVSI EEGSCTQRML
910 920 930 940 950
KILPRVAGPR LAGDVQTVIA AARRGDWTDH DGHVTAGGIP LLENEYQLVA
960 970 980 990 1000
GAQDSKNSQP LPFGGSVTLD TRIDETLRSE GVARDTVRQI QIARKEKDLN
1010 1020 1030 1040 1050
ITDRISLEVC VPDEQVKNNL LAFSELICKE TLCDRLDILV KKGIDGITVS

LEKFRQ
Length:1,056
Mass (Da):119,751
Last modified:June 1, 2003 - v1
Checksum:iD1D531A27E790967
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX251411 Genomic DNA. Translation: CAD66961.1.
RefSeqiNP_789223.1. NC_004551.1.

Genome annotation databases

EnsemblBacteriaiCAD66961; CAD66961; TW287.
GeneIDi1064576.
KEGGitws:TW287.
PATRICi23999027. VBITroWhi42739_0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX251411 Genomic DNA. Translation: CAD66961.1 .
RefSeqi NP_789223.1. NC_004551.1.

3D structure databases

ProteinModelPortali Q83I16.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218496.TW287.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD66961 ; CAD66961 ; TW287 .
GeneIDi 1064576.
KEGGi tws:TW287.
PATRICi 23999027. VBITroWhi42739_0350.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW08/27.

Entry informationi

Entry nameiSYI_TROW8
AccessioniPrimary (citable) accession number: Q83I16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3