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Q83I16

- SYI_TROW8

UniProt

Q83I16 - SYI_TROW8

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Tropheryma whipplei (strain TW08/27) (Whipple's bacillus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei635 – 6351ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TW287
    OrganismiTropheryma whipplei (strain TW08/27) (Whipple's bacillus)
    Taxonomic identifieri218496 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
    ProteomesiUP000001413: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10561056Isoleucine--tRNA ligasePRO_0000098570Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi218496.TW287.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83I16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi63 – 7311"HIGH" regionAdd
    BLAST
    Motifi632 – 6365"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q83I16-1 [UniParc]FASTAAdd to Basket

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    MCDQGEVSSQ NSSDYKEQRP TPRPNLPKIE ESVLAFWSSD KTFEASLEQR     50
    QHGKRWVFYD GPPFANGLPH FGHLLTGYIK DAIPRYQTMR GQYVPRVFGW 100
    DTHGLPAELE AMKRLGITEK SQIESMGIAS FNEAARKSVL TYVDQWEEYV 150
    NRQARWVDFK NGYKTLDLDY MESVLWAFKT LYKKGVIYEG YKVLPYCWND 200
    QTPLSNHELR MDDEVYKQRL DDSLTVTFPL IGQKAKTCGL DGVAALAWTT 250
    TPWTLPSNMA LIVSPNVEYV VVSSARQNSN SDFLLCKSSL DSYAECLGYE 300
    SGQDARASIR RTLLGKEIEG IHYKPLFDYY ADLHNAFTIL SDNYVDVTEG 350
    TGIVHASPAH GEDDKRVCDA FGVPTVVSIN DAACFTDVIS NYAGMHIFDA 400
    NAVIRSDLSR DGRILRHESY KHSYPHCWRC RSPLIYKAVT SWFFRITDSV 450
    NRMLELNQQI NWVPKSVKNG QFAKWLSSAK DWSISRTRYW GTPIPVWKSD 500
    NPEYPRIDCY GSLKELEDDF GIKLTDLHRP EIDRLTRPNP DDPTGASTMR 550
    RVPDVLDVWF DAASMPFAQL HYPFENIERF EANKSADFIV EYAGQIRGWF 600
    YLLHAMSTAL FDGVAFKNAI CHGIVLGDDG QKASKSLRNY PDVYDVFENE 650
    GSDAVRWYLI SSSILRGGSL IVSRKKIQDA IRQYITPLWS SWYFFHIYSE 700
    AARPGGYKAR FSVDSQDILD RYILSKTGLL VEDVTRFMDS FDMASAALQL 750
    RDFVAVLTNW YIRRSRDRFW DGSDTGAFDT LYTVLETLCR LGAVFVPMVS 800
    EHVYKCLTNS RSVHLSDWPD VATFPNETGL VETMDRVRKI CSTGLSLRKR 850
    LGIKARQPLS SAHIRVAQVG SLAQYKDIIS GELNVKTVSI EEGSCTQRML 900
    KILPRVAGPR LAGDVQTVIA AARRGDWTDH DGHVTAGGIP LLENEYQLVA 950
    GAQDSKNSQP LPFGGSVTLD TRIDETLRSE GVARDTVRQI QIARKEKDLN 1000
    ITDRISLEVC VPDEQVKNNL LAFSELICKE TLCDRLDILV KKGIDGITVS 1050
    LEKFRQ 1056
    Length:1,056
    Mass (Da):119,751
    Last modified:June 1, 2003 - v1
    Checksum:iD1D531A27E790967
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX251411 Genomic DNA. Translation: CAD66961.1.
    RefSeqiNP_789223.1. NC_004551.1.

    Genome annotation databases

    EnsemblBacteriaiCAD66961; CAD66961; TW287.
    GeneIDi1064576.
    KEGGitws:TW287.
    PATRICi23999027. VBITroWhi42739_0350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX251411 Genomic DNA. Translation: CAD66961.1 .
    RefSeqi NP_789223.1. NC_004551.1.

    3D structure databases

    ProteinModelPortali Q83I16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 218496.TW287.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD66961 ; CAD66961 ; TW287 .
    GeneIDi 1064576.
    KEGGi tws:TW287.
    PATRICi 23999027. VBITroWhi42739_0350.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TW08/27.

    Entry informationi

    Entry nameiSYI_TROW8
    AccessioniPrimary (citable) accession number: Q83I16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3