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Q83HV5 (SYL_TROW8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:TW385
OrganismTropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]
Taxonomic identifier218496 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma

Protein attributes

Sequence length806 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 806806Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152110

Regions

Motif54 – 6411"HIGH" region HAMAP-Rule MF_00049
Motif571 – 5755"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83HV5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 0A85A3718E1609F2

FASTA80692,374
        10         20         30         40         50         60 
MHALRALDTL CENMEYNFRA LEEKWAPIWE RDRLFEVDEN DSETPRKYVL DMFSYPSGDL 

        70         80         90        100        110        120 
HMGHAETYAY GDFIARYWRH RGYNVLHPVG WDSFGLPAEN AAIKHGSDPK VWTYRNIDQQ 

       130        140        150        160        170        180 
ARSMRLYAAS FDWSRRLHTS DPEYYRWNQW LFLKLYKHGL AYRKKAWVNW DPSDRTVLAN 

       190        200        210        220        230        240 
EQVLPDGTSE RSGALVVKKK LTQWFLRITA YADRLLDDLS MLENNWPERV ITMQRNWIGR 

       250        260        270        280        290        300 
SEGVSIEFNI PTLKRPVMVF TTRPETIFGV TYLALAFDSE VTEELASKSG VLGELLELRH 

       310        320        330        340        350        360 
NIDKTHESVR GLDLKSFAIH PLTGQSIPIF AASYILSDYA KGAVMSVPGH DTRDERFAVR 

       370        380        390        400        410        420 
YNLPIVKIME DNRLISSGKY SGQSVTQARE NITRDLCAKS LGRREISYRL RDWLISRQRY 

       430        440        450        460        470        480 
WGTPIPILYD SNGSEIPVEE DDLPVLLPDS EGIDLTPSGL SPLGGIHDWV NLHKAGSLFR 

       490        500        510        520        530        540 
RDTDTMDTFF DSSWYFLRYL NPDCDTAPFT LEKAKKWGPV DQYCGGVEHA VLHLLYARFI 

       550        560        570        580        590        600 
TKFLYDIGFV DFKEPFLRLI NQGMVVLNGA KMSKSKGNIV EFSKEVSQHG VDVIRFALIF 

       610        620        630        640        650        660 
SGPPEEDIDW KDVSMTGAAR FLSRCIQTAK EVPKRTADLS LGDIELRKHT HSLLNDIDWL 

       670        680        690        700        710        720 
VDSYRFNVIA ARLMDLLNIT RKKIQTIGAD NPAIREAIET IAIALDMFSP YTAEEMWEIL 

       730        740        750        760        770        780 
GNKYSVSKAL FPEVDTTFLE QKTTCAIVQI DGRLRGRLNV LTNITTEQLV HSARSLPAIE 

       790        800 
HALSGRSVKR VICVPPKLVN FVVEPK 

« Hide

References

[1]"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei."
Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A.
Lancet 361:637-644(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TW08/27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX251411 Genomic DNA. Translation: CAD67056.1.
RefSeqNP_789318.1. NC_004551.1.

3D structure databases

ProteinModelPortalQ83HV5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING218496.TW385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD67056; CAD67056; TW385.
GeneID1064671.
KEGGtws:TW385.
PATRIC23999241. VBITroWhi42739_0450.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMALHMGHAE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_TROW8
AccessionPrimary (citable) accession number: Q83HV5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries