Q83HS8 (SYC1_TROW8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase 1
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase 1
Short name=CysRS 1

Gene names

Name:	cysS1
Ordered Locus Names:	TW423

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Cysteine--tRNA ligase 1 HAMAP MF_00041		PRO_0000159512
Regions
	Motif	29 – 39	11	"HIGH" region HAMAP MF_00041
	Motif	267 – 271	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	27	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	270	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83HS8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6C6105AAE63BF30D	FASTA	466	52,787
	10 20 30 40 50 60 MTLNLYDTLS RRIVTLDCRD RVELYVCGPT VQSPPHIGHM RSGVVYDCLR RWLEYKGLPV 70 80 90 100 110 120 LYVRNITDID DKILASARST ETGETWWQIA YRVSGLFNEA YKALFVKPPD YEPLVTAHIP 130 140 150 160 170 180 DIIKAIEILI QKNVAYRAMD GSGNVFFSID KHPSYGELTD QKDLLIDDCI TPGKRDPRDF 190 200 210 220 230 240 TLWKEKKDTD PDLAFWESPW GPGRPGWHIE CSVMATKYLG TRFAIHGGGV DLRFPHHENE 250 260 270 280 290 300 LAQARALGAH FADIWMHTGA VNVEGIKMSK SFGNSVLVQD ALSKVSPSAL RYYFLTAHYR 310 320 330 340 350 360 STLNYTENSL SQACNTWNKL QGCIYRVYDY LEREGYENDF CVSQLNTDFS TSLDNDLNIP 370 380 390 400 410 420 EALAIVHNKV REMNRLVDTQ ADMQYLGNTL SEVVEMLSIL CPIDHKITYT SGAQDNSKAL 430 440 450 460 LQVLLEARDR ARSKGDFYTS DLLRELLAEA DISVSDGHVS YGSPRG

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW08/27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX251411 Genomic DNA. Translation: CAD67093.1.
RefSeq	NP_789355.1. NC_004551.1.
3D structure databases
ProteinModelPortal	Q83HS8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83HS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064266.
GenomeReviews	Gene locus TW423 in contig BX072543_GR.
KEGG	tws:TW423.
PATRIC	23999321. VBITroWhi42739_0489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	NVFDIHG.
PhylomeDB	Q83HS8.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	TWHI218496:TW0400-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC1_TROW8

Accession

Primary (citable) accession number: Q83HS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program