Reviewed, UniProtKB/Swiss-Prot Q83HQ8 (SYC2_TROW8)

Names and origin

Protein names

Recommended name:
    Putative cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	TW456

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Putative cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000159513
Regions
	Motif	29 – 39	11	"HIGH" region HAMAP MF_00041
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00041
Sites
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83HQ8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2754844783A8B17D	FASTA	450	51,274
	10 20 30 40 50 60 MYTFEVFDSR TRSIVKLEDC LLRLYVCGIT PYKSTHLGHA FTYVGFDTLF RLALDSGRDV 70 80 90 100 110 120 LYIQNISDID EPLFQYAEKV GIHYKDLART QTKRFFEDMH TLECLPPGYV IPVSKVLDGI 130 140 150 160 170 180 KTGIEGLISR NMAYKLPNGD VYFDSTLTDP GKMFCFDRKT AMSLMLETDT GKNPFDPLLW 190 200 210 220 230 240 RGRGAEPQWE ASFGAGRPAW HISCAVLSNL QTQYENVLHI YGGGRDLAFP HHEFTNVLSK 250 260 270 280 290 300 LIRAPKDNKQ QDTVQDVFMH TGLVSYMGDK MSKSKGNLVF ISQLREQCEK IGLHHSVIRL 310 320 330 340 350 360 ALLQRHYRED WEWQDECLDR AASRFRLWKS ALQEYIGAKG IRSTADQNKG TQGAWERIHG 370 380 390 400 410 420 GVFDSNFDHR QPIHPKHSPQ MRDYSEHGSA GQNGTDLDLS LYQAIRFHLC NDLDTPKALD 430 440 450 AVDSYARKGT ITIPEARAVE KLLGIPLTRV

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX251411 Genomic DNA. Translation: CAD67124.1.
RefSeq	NP_789386.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83HQ8.
Genome annotation databases
GeneID	1064297.
GenomeReviews	Gene locus TW456 in contig BX072543_GR.
KEGG	tws:TW456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q83HQ8.
OMA	HYVQNVT.
Family and domain databases
HAMAP	MF_00041. Divergent sequence. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_TROW8

Accession

Primary (citable) accession number: Q83HQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)