Q83HI1 (SYP_TROW8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	TW588

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Proline--tRNA ligase HAMAP MF_01569		PRO_0000248806

Sequences

	Sequence		Length	Mass (Da)
	Q83HI1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9FD27DE136C6E326	FASTA	601	67,005
	10 20 30 40 50 60 MITKVSDFLF RTFREDPATT ESRGYGFLLR AGYIRQTGSG IFSWMPLGLK VRHKIENIIR 70 80 90 100 110 120 YEMRQVNAIE VLFPALFSAD LFKQSGRWSE YGDDIFRLKD RRQGDYLLAP THEEAFTQMM 130 140 150 160 170 180 KEICTSYRDL PRTVYQIQDK YRDELRPRAG LLRSREFSMK DAYSFDLDEK GLRQSYEAQK 190 200 210 220 230 240 RAYKKIFDRL EIDYVIVKAN AGAMGGSVSE EFLHPTEMGD DTFVVTADGS AFNAEVYVTL 250 260 270 280 290 300 PGPAIDYSNA PEAEDCETPG VISIPDLVNH MNSSGRFIGR VIESSDCLKC LLFRIEYAEV 310 320 330 340 350 360 QNGNPGNLVV KKILERGFEY IGFLVPGDRN VDLKRAQVAL SPLTIEPADN RVFECNPSFV 370 380 390 400 410 420 RGSIGPGLSG VFYCADPRVS SGSSWIIGAN RPGVHRIGAI AGRDFSFDCT LDVSSIKTGD 430 440 450 460 470 480 KSEWGPVTVK RGIEIGHLFQ LGLKYSNALG LKVLDKDGYN KAVFMGSYGI GVSRLFALIA 490 500 510 520 530 540 EKNCDERGLK WPAVLAPFDL HVVLLSSARA ELIDSLTDCG LDVLVDDRRV SPGVKFTDAQ 550 560 570 580 590 600 LIGVPKIIVI GDKTRGEDVE VWDRANDQRT VLPLKEMIQG VIQRSDTGGC TERCTGVCPT R

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW08/27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX251412 Genomic DNA. Translation: CAD67254.1.
RefSeq	NP_789516.1. NC_004551.1.
3D structure databases
ProteinModelPortal	Q83HI1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83HI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064792.
GenomeReviews	Gene locus TW588 in contig BX072543_GR.
KEGG	tws:TW588.
PATRIC	23999801. VBITroWhi42739_0721.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
PhylomeDB	Q83HI1.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	TWHI218496:TW0561-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_TROW8

Accession

Primary (citable) accession number: Q83HI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program