Q83HB7 (Q83HB7_TROW8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL CAD67374.1
Ordered Locus Names:	TW715

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q83HB7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0F7061974F3E7FDC	FASTA	841	94,339
	10 20 30 40 50 60 MKKLVIVESP TKVSSVGRYL GDGYVVMSSV GHIRDLPAPS GLTPDLKRSA HAQFAVNIEN 70 80 90 100 110 120 DFEPYYVVLE NKKKVVEELQ SALNNSDELL IATDGDREGE AIAWHLVQVL KPTVPVRRLV 130 140 150 160 170 180 FHEITREAIA YALQHPRPID ENLVDAQEAR RILDRLYGYE LSPVLWKKVR PGLSAGRVQS 190 200 210 220 230 240 PATRLLVDRE RERINFKSAE YQAVTADFQV DSDVFSASLT LFGERKIATG RDFDNLANLK 250 260 270 280 290 300 TDALVLDKNL TDRILEELKS KGALDVEAID SKPYSRNPYA PFITSTLQQD AAHKLGFSAK 310 320 330 340 350 360 RTMRTAQQLY EKGFITYMRT DSPRLSLQAI SAARAQVELL YGREFLPDSP RTYGAKAKNA 370 380 390 400 410 420 QEAHEAIRPT GEVFERPESI ESVLSVDCMR LYDLIWRRTI ASQMVSARGE TVTLTLSARI 430 440 450 460 470 480 PERSIFVLSG TVLHEKGFLK VYEGLQDLNS KLLPNLQVGD QVTLVDVRAV GHTTLPPPRY 490 500 510 520 530 540 TEASLVKVLE ELGLGRPSTY ALILRTIQER GYAHLRNRML VPDWLAFSVV NLLENFFSRL 550 560 570 580 590 600 IDYDFTANLE DQLDEIALGS QNRSDWLRKF YFGDDNLPGL KEIISGAKDI DARAINSIPM 610 620 630 640 650 660 VGATLRVGRY GPYLEVGDKR FSIPHDVYPD ALTPEKIHEL VNQPESPVRE LGAHPETGKL 670 680 690 700 710 720 VVLKSGRFGP YIEEVSDEKN VRRSSLFKNM DPEEVNLDVA VKLLEIPRVI GVNQENGEKV 730 740 750 760 770 780 IACNGRFGPY IKCGKETRPL DSEDDILSVT LDEALAILSA PKVRRSRVLM EFPTDDPVTR 790 800 810 820 830 840 RAVQIKSGRF GPYVTDGVTN ATLDNTSWED VTFDEAVSLL AEKRQRVDGD LDQETAEKIH V

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX251412 Genomic DNA. Translation: CAD67374.1.
RefSeq	NP_789636.1. NC_004551.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortal	Q83HB7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83HB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064915.
GenomeReviews	Gene locus TW715 in contig BX072543_GR.
KEGG	tws:TW715.
PATRIC	24000115. VBITroWhi42739_0866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	Q83HB7.
ProtClustDB	CLSK230235.
Enzyme and pathway databases
BioCyc	TWHI218496:TW0681-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q83HB7_TROW8

Accession

Primary (citable) accession number: Q83HB7

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)