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Q83GU0 (Q83GU0_TROWT) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168 RuleBase RU003777
EC=2.4.2.29 HAMAP-Rule MF_00168 RuleBase RU003777
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168
Ordered Locus Names:TWT_152 EMBL AAO44249.1
OrganismTropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP] EMBL AAO44249.1
Taxonomic identifier203267 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma ›

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1081Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3391Zinc By similarity HAMAP-Rule MF_00168
Metal binding3411Zinc By similarity HAMAP-Rule MF_00168
Metal binding3441Zinc By similarity HAMAP-Rule MF_00168
Metal binding3701Zinc By similarity HAMAP-Rule MF_00168
Binding site1091Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
Q83GU0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 2D3B3F2534E97EC7

FASTA40445,689
        10         20         30         40         50         60 
MLDLPVWGNY ITGWSIGRWS MFSVLKQTEY GRTGIISTCN GQIHTPAFIP VATKASIKGV 

        70         80         90        100        110        120 
LPEQMKQLGA QGLLANAYHL YLRPGPDTIA AAGGLSRFMS WNGPTFTDSG GFQVMSLGRG 

       130        140        150        160        170        180 
GKKVISMEVP DNTWESNIRK TSLIRIDDQG VWFRSHIDGS QHRFTPEESI RVQNKIGADI 

       190        200        210        220        230        240 
IFAFDQLTTL DDSRAVQEYA LERTRLWAER CLNQHSKEDQ ALFAVIQGAN YRDLREKACR 

       250        260        270        280        290        300 
DLGQMGFDGF GIGGALEKSR IREIITWCTR KLPEEKPRHL LGISEPNDLM NAIEAGIDTF 

       310        320        330        340        350        360 
DCVSPTRVAR NTAFYTPSGR KNLRRKMYRN DFSPLVEGCA CYACSNFTRA YIHHLFRAKE 

       370        380        390        400 
MCAATLTSIH NEYFIVSMVD GARKAIDEGY FREYKGKILR EYYK

« Hide

References

[1]"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Twist.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014184 Genomic DNA. Translation: AAO44249.1.
RefSeqNP_787280.1. NC_004572.3.

3D structure databases

HSSPHSSP built from PDB template 1P0E based on UniProtKB P28720.
ProteinModelPortalQ83GU0.
ModBaseSearch...

Protein-protein interaction databases

STRING203267.TWT152.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO44249; AAO44249; TWT_152.
GeneID1482417.
KEGGtwh:TWT152.
PATRIC23996637. VBITroWhi72342_0207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBCLSK899985.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ83GU0_TROWT
AccessionPrimary (citable) accession number: Q83GU0
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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