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Q83GR0

- GLMU_TROWT

UniProt

Q83GR0 - GLMU_TROWT

Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
    UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241UDP-GlcNAcUniRule annotation
    Binding sitei75 – 751UDP-GlcNAcUniRule annotation
    Metal bindingi165 – 1651MagnesiumUniRule annotation
    Binding sitei201 – 2011UDP-GlcNAc; via amide nitrogenUniRule annotation
    Binding sitei216 – 2161UDP-GlcNAcUniRule annotation
    Binding sitei230 – 2301UDP-GlcNAcUniRule annotation
    Metal bindingi367 – 3671MagnesiumUniRule annotation
    Binding sitei367 – 3671UDP-GlcNAcUniRule annotation
    Binding sitei472 – 4721Acetyl-CoA; amide nitrogenUniRule annotation
    Binding sitei490 – 4901Acetyl-CoAUniRule annotation
    Active sitei502 – 5021Proton acceptorUniRule annotation
    Binding sitei505 – 5051Acetyl-CoAUniRule annotation
    Binding sitei516 – 5161Acetyl-CoAUniRule annotation
    Binding sitei562 – 5621Acetyl-CoA; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell morphogenesis Source: UniProtKB-HAMAP
    2. lipid A biosynthetic process Source: UniProtKB-UniPathway
    3. lipopolysaccharide biosynthetic process Source: InterPro
    4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    5. regulation of cell shape Source: UniProtKB-KW
    6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciTWHI203267:GJDK-201-MONOMER.
    UniPathwayiUPA00113; UER00532.
    UPA00113; UER00533.
    UPA00973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein GlmUUniRule annotation
    Including the following 2 domains:
    UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
    Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
    Gene namesi
    Name:glmUUniRule annotation
    Ordered Locus Names:TWT_188
    OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
    Taxonomic identifieri203267 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
    ProteomesiUP000002200: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595Bifunctional protein GlmUPRO_0000244320Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203267.TWT188.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83GR0.
    SMRiQ83GR0. Positions 5-45.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 369369PyrophosphorylaseUniRule annotationAdd
    BLAST
    Regioni10 – 134UDP-GlcNAc bindingUniRule annotation
    Regioni81 – 822UDP-GlcNAc bindingUniRule annotation
    Regioni370 – 39021LinkerUniRule annotationAdd
    BLAST
    Regioni391 – 595205N-acetyltransferaseUniRule annotationAdd
    BLAST
    Regioni525 – 5262Acetyl-CoA bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
    In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1207.
    KOiK04042.
    OMAiDCVTNQD.
    OrthoDBiEOG6Z6FQZ.

    Family and domain databases

    Gene3Di3.90.550.10. 3 hits.
    HAMAPiMF_01631. GlmU.
    InterProiIPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 3 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q83GR0-1 [UniParc]FASTAAdd to Basket

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    MKSDLAIVIL AAGRGTRMRS STPKVLHNIA GLPMVAHVLR GAQLLKPCKT    50
    IVVFRDVRVE QYIRNTFPDV LTVAQSDALY GTGFGVFSAI PWIRSESYGN 100
    TYPNTRHESH AEYELDACDL DTCNPASDRL NDQESLKGGR HIHTKSGDVT 150
    NNKPFPSRVL ILYADVPLVP FQLLEELVRK PLKQAVGAIV TTHLDNPKGY 200
    GRVMRDNLGS IAKIIEDSNI LPEQSINEVN TGVGIFDTEY LQDALNKLLK 250
    CHIAHSPNQD CVTNQDCVTN QDCVTNQDCV TNQDCVPAAH TEAHVLSPKV 300
    IVTEQIHKKA ESEHQHAGQW MCRQDTVGAQ NTKEEMRLTD IVEYFYNNGL 350
    RVNSITTSDS ELLLGVNNRV QLAKTEKILN DQIIKRWQLY GVTIKSPETT 400
    WIDSTVQLSE DVLILPGCIL SGRTRIEEGA VIGPFATISD SFIGKNTIVK 450
    RAEIIDARIE EGAVIGPFAF IRPGTVIGKD SKVGTFVEIK QSNIGPESKV 500
    PHLSYIGDAN IGSHVNIGAG NIFANYDGKL KHETCIDDGV KTGAGNVFVA 550
    PVKVGRGAYT GAGSVIRDDI EEGALSLTEL KQKTIPKWAE NRGDG 595
    Length:595
    Mass (Da):65,166
    Last modified:June 27, 2006 - v2
    Checksum:i6F145B2ED00519E7
    GO

    Sequence cautioni

    The sequence AAO44285.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014184 Genomic DNA. Translation: AAO44285.1. Different initiation.
    RefSeqiNP_787316.1. NC_004572.3.

    Genome annotation databases

    EnsemblBacteriaiAAO44285; AAO44285; TWT_188.
    GeneIDi1482456.
    KEGGitwh:TWT188.
    PATRICi23996739. VBITroWhi72342_0255.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014184 Genomic DNA. Translation: AAO44285.1 . Different initiation.
    RefSeqi NP_787316.1. NC_004572.3.

    3D structure databases

    ProteinModelPortali Q83GR0.
    SMRi Q83GR0. Positions 5-45.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203267.TWT188.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO44285 ; AAO44285 ; TWT_188 .
    GeneIDi 1482456.
    KEGGi twh:TWT188.
    PATRICi 23996739. VBITroWhi72342_0255.

    Phylogenomic databases

    eggNOGi COG1207.
    KOi K04042.
    OMAi DCVTNQD.
    OrthoDBi EOG6Z6FQZ.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00532 .
    UPA00113 ; UER00533 .
    UPA00973 .
    BioCyci TWHI203267:GJDK-201-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 3 hits.
    HAMAPi MF_01631. GlmU.
    InterProi IPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF00132. Hexapep. 3 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
      Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
      Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Twist.

    Entry informationi

    Entry nameiGLMU_TROWT
    AccessioniPrimary (citable) accession number: Q83GR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3