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Q83GR0

- GLMU_TROWT

UniProt

Q83GR0 - GLMU_TROWT

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241UDP-GlcNAcUniRule annotation
Binding sitei75 – 751UDP-GlcNAcUniRule annotation
Metal bindingi165 – 1651MagnesiumUniRule annotation
Binding sitei201 – 2011UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei216 – 2161UDP-GlcNAcUniRule annotation
Binding sitei230 – 2301UDP-GlcNAcUniRule annotation
Metal bindingi367 – 3671MagnesiumUniRule annotation
Binding sitei367 – 3671UDP-GlcNAcUniRule annotation
Binding sitei472 – 4721Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei490 – 4901Acetyl-CoAUniRule annotation
Active sitei502 – 5021Proton acceptorUniRule annotation
Binding sitei505 – 5051Acetyl-CoAUniRule annotation
Binding sitei516 – 5161Acetyl-CoAUniRule annotation
Binding sitei562 – 5621Acetyl-CoA; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-201-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:TWT_188
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000002200: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Bifunctional protein GlmUPRO_0000244320Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi203267.TWT188.

Structurei

3D structure databases

ProteinModelPortaliQ83GR0.
SMRiQ83GR0. Positions 5-45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 369369PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni10 – 134UDP-GlcNAc bindingUniRule annotation
Regioni81 – 822UDP-GlcNAc bindingUniRule annotation
Regioni370 – 39021LinkerUniRule annotationAdd
BLAST
Regioni391 – 595205N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni525 – 5262Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
KOiK04042.
OMAiDCVTNQD.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 3 hits.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q83GR0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSDLAIVIL AAGRGTRMRS STPKVLHNIA GLPMVAHVLR GAQLLKPCKT
60 70 80 90 100
IVVFRDVRVE QYIRNTFPDV LTVAQSDALY GTGFGVFSAI PWIRSESYGN
110 120 130 140 150
TYPNTRHESH AEYELDACDL DTCNPASDRL NDQESLKGGR HIHTKSGDVT
160 170 180 190 200
NNKPFPSRVL ILYADVPLVP FQLLEELVRK PLKQAVGAIV TTHLDNPKGY
210 220 230 240 250
GRVMRDNLGS IAKIIEDSNI LPEQSINEVN TGVGIFDTEY LQDALNKLLK
260 270 280 290 300
CHIAHSPNQD CVTNQDCVTN QDCVTNQDCV TNQDCVPAAH TEAHVLSPKV
310 320 330 340 350
IVTEQIHKKA ESEHQHAGQW MCRQDTVGAQ NTKEEMRLTD IVEYFYNNGL
360 370 380 390 400
RVNSITTSDS ELLLGVNNRV QLAKTEKILN DQIIKRWQLY GVTIKSPETT
410 420 430 440 450
WIDSTVQLSE DVLILPGCIL SGRTRIEEGA VIGPFATISD SFIGKNTIVK
460 470 480 490 500
RAEIIDARIE EGAVIGPFAF IRPGTVIGKD SKVGTFVEIK QSNIGPESKV
510 520 530 540 550
PHLSYIGDAN IGSHVNIGAG NIFANYDGKL KHETCIDDGV KTGAGNVFVA
560 570 580 590
PVKVGRGAYT GAGSVIRDDI EEGALSLTEL KQKTIPKWAE NRGDG
Length:595
Mass (Da):65,166
Last modified:June 27, 2006 - v2
Checksum:i6F145B2ED00519E7
GO

Sequence cautioni

The sequence AAO44285.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44285.1. Different initiation.
RefSeqiNP_787316.1. NC_004572.3.

Genome annotation databases

EnsemblBacteriaiAAO44285; AAO44285; TWT_188.
GeneIDi1482456.
KEGGitwh:TWT188.
PATRICi23996739. VBITroWhi72342_0255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44285.1 . Different initiation.
RefSeqi NP_787316.1. NC_004572.3.

3D structure databases

ProteinModelPortali Q83GR0.
SMRi Q83GR0. Positions 5-45.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203267.TWT188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO44285 ; AAO44285 ; TWT_188 .
GeneIDi 1482456.
KEGGi twh:TWT188.
PATRICi 23996739. VBITroWhi72342_0255.

Phylogenomic databases

eggNOGi COG1207.
KOi K04042.
OMAi DCVTNQD.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci TWHI203267:GJDK-201-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 3 hits.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
    Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
    Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Twist.

Entry informationi

Entry nameiGLMU_TROWT
AccessioniPrimary (citable) accession number: Q83GR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 26, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3