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Q83GR0

- GLMU_TROWT

UniProt

Q83GR0 - GLMU_TROWT

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Protein

Bifunctional protein GlmU

Gene
glmU, TWT_188
Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241UDP-GlcNAc By similarity
Binding sitei75 – 751UDP-GlcNAc By similarity
Metal bindingi165 – 1651Magnesium By similarity
Binding sitei201 – 2011UDP-GlcNAc; via amide nitrogen By similarity
Binding sitei216 – 2161UDP-GlcNAc By similarity
Binding sitei230 – 2301UDP-GlcNAc By similarity
Metal bindingi367 – 3671Magnesium By similarity
Binding sitei367 – 3671UDP-GlcNAc By similarity
Binding sitei472 – 4721Acetyl-CoA; amide nitrogen By similarity
Binding sitei490 – 4901Acetyl-CoA By similarity
Active sitei502 – 5021Proton acceptor By similarity
Binding sitei505 – 5051Acetyl-CoA By similarity
Binding sitei516 – 5161Acetyl-CoA By similarity
Binding sitei562 – 5621Acetyl-CoA; via amide nitrogen By similarity

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. lipid A biosynthetic process Source: UniProtKB-UniPathway
  3. lipopolysaccharide biosynthetic process Source: InterPro
  4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  5. regulation of cell shape Source: UniProtKB-KW
  6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-201-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmU
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylase (EC:2.7.7.23)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferase
Glucosamine-1-phosphate N-acetyltransferase (EC:2.3.1.157)
Gene namesi
Name:glmU
Ordered Locus Names:TWT_188
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000002200: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Bifunctional protein GlmUUniRule annotationPRO_0000244320Add
BLAST

Interactioni

Subunit structurei

Homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi203267.TWT188.

Structurei

3D structure databases

ProteinModelPortaliQ83GR0.
SMRiQ83GR0. Positions 5-45.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 369369Pyrophosphorylase By similarityAdd
BLAST
Regioni10 – 134UDP-GlcNAc binding By similarity
Regioni81 – 822UDP-GlcNAc binding By similarity
Regioni370 – 39021Linker By similarityAdd
BLAST
Regioni391 – 595205N-acetyltransferase By similarityAdd
BLAST
Regioni525 – 5262Acetyl-CoA binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.
In the C-terminal section; belongs to the transferase hexapeptide repeat family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
KOiK04042.
OMAiDCVTNQD.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 3 hits.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q83GR0-1 [UniParc]FASTAAdd to Basket

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MKSDLAIVIL AAGRGTRMRS STPKVLHNIA GLPMVAHVLR GAQLLKPCKT    50
IVVFRDVRVE QYIRNTFPDV LTVAQSDALY GTGFGVFSAI PWIRSESYGN 100
TYPNTRHESH AEYELDACDL DTCNPASDRL NDQESLKGGR HIHTKSGDVT 150
NNKPFPSRVL ILYADVPLVP FQLLEELVRK PLKQAVGAIV TTHLDNPKGY 200
GRVMRDNLGS IAKIIEDSNI LPEQSINEVN TGVGIFDTEY LQDALNKLLK 250
CHIAHSPNQD CVTNQDCVTN QDCVTNQDCV TNQDCVPAAH TEAHVLSPKV 300
IVTEQIHKKA ESEHQHAGQW MCRQDTVGAQ NTKEEMRLTD IVEYFYNNGL 350
RVNSITTSDS ELLLGVNNRV QLAKTEKILN DQIIKRWQLY GVTIKSPETT 400
WIDSTVQLSE DVLILPGCIL SGRTRIEEGA VIGPFATISD SFIGKNTIVK 450
RAEIIDARIE EGAVIGPFAF IRPGTVIGKD SKVGTFVEIK QSNIGPESKV 500
PHLSYIGDAN IGSHVNIGAG NIFANYDGKL KHETCIDDGV KTGAGNVFVA 550
PVKVGRGAYT GAGSVIRDDI EEGALSLTEL KQKTIPKWAE NRGDG 595
Length:595
Mass (Da):65,166
Last modified:June 27, 2006 - v2
Checksum:i6F145B2ED00519E7
GO

Sequence cautioni

The sequence AAO44285.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014184 Genomic DNA. Translation: AAO44285.1. Different initiation.
RefSeqiNP_787316.1. NC_004572.3.

Genome annotation databases

EnsemblBacteriaiAAO44285; AAO44285; TWT_188.
GeneIDi1482456.
KEGGitwh:TWT188.
PATRICi23996739. VBITroWhi72342_0255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014184 Genomic DNA. Translation: AAO44285.1 . Different initiation.
RefSeqi NP_787316.1. NC_004572.3.

3D structure databases

ProteinModelPortali Q83GR0.
SMRi Q83GR0. Positions 5-45.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203267.TWT188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO44285 ; AAO44285 ; TWT_188 .
GeneIDi 1482456.
KEGGi twh:TWT188.
PATRICi 23996739. VBITroWhi72342_0255.

Phylogenomic databases

eggNOGi COG1207.
KOi K04042.
OMAi DCVTNQD.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci TWHI203267:GJDK-201-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 3 hits.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
    Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
    Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Twist.

Entry informationi

Entry nameiGLMU_TROWT
AccessioniPrimary (citable) accession number: Q83GR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: June 11, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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